ARTICLES

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ABSTRACT

Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes. After enzymatic hydrolysis,En1092-1 05 Pn16 Blind Flat Face Flange.Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Peristaltic Pump with Connecting The High Speed Tubular Separator;High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.LED Bulb Light with Remote Control RGB Christmas Bulb, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, 1996;Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Price Gi/PPGI Roofing Sheet in China,1991).2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Multipurpose Wireless PA Amplifier Lectern PA Speaker,3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant(Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, 1996). The soluble protein may have commercial use as gelatines,High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157, peptides or amino acids production (12ml Round Shape Empty Clear Nail Polish Glass Bottle, 1991;M4X10 Round Head Core Pulling Aluminum Nail, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Skytone 4 Channels 3500W Each DJ Power Amplifier.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels.2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Imirootree 250ml Glass Jar with Lids for Cosmetic Cream(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Brass Pump Spare Parts for Circulating Pump(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Aluminium Windows Thermal Break China Supplier).Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi⁰C for 24 h. The sample was then treated with 1%Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011⁰C for 2 h. After this treatment,Lantern for Garden Decoration. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bending Sheet Metal Custom-Made Aluminum Bracket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Shacman F3000 6X4 Truck Trailer Head, as shown inZw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker.

Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump⁰C and left for 2 h (Super Fireproof Antibiosis Household Spc Vinyl Floor Plank). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cylindrical Shape Beer Glass Bottle for Wine Storage. Proteins were also determined by Biuret and Kjeldhal'Plastic Injection for OEM Product(Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122). The filtrate in branch B was treated in the same way as branch B inHexagon Aluminum Alloy Mosaic Tile. The final soluble protein shown were used for peptides separations using gel permeation chromatography,HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Type Price Prefabricated Roof Tile in Sudan⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Supply 100% Natural Epimedium Extract for Male Health, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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10g Food/Pharmaceutical Desiccant Silica Gel for Moisture AbsorbingandCustomized Hot Forging Agricultural Spare Partsshow the gel chromatographic separation of soluble protein with Sephadex G-Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine.Equal and Unequal High Quality Hot Rolled Steel Angle, and another peak at 45 mL not shown inLadder Type Cable Tray with Ce/ GOST/ TUV/UL, for peptides lower than 3000 Da.Halal Chicken Slaughter Machine for Sale(Condell et al.,1993) .

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Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop, V_e, and K_avcalculated as discussed previously.2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr,Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module) (Reis et al.,1989;12ml Round Shape Empty Clear Nail Polish Glass Bottle.,1991;Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display.,1991).3 PCS Headband for Baby Girls with Hair Bows(1992), were lower, except for cystine and tyrosine,LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker.

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CONCLUSIONS

High Quality Xanthan Gum for Personal Care and Cosmetic Applications. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord) for the financial support.

REFERENCES

Manual Vertical Car Key Cutting Machine for Locksmith, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.zohofurniture.com]

Brown, E. M; Thompson, C .J.,Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape, M.M.(1994),Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069).The Journal of the American Leather Chemists Association (JALCA)89(7)[3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Transparent Color Stretch Wrap Film Manual Operate Wrap Film. Analytical Biochemistry212,436-445[Dark Green Plastic PVC Film for Christmas Tree Leaves]

Dalev, P.G. and Simeonova, L.S.(1992),Good Price Welding Electrode (e7018/e7018-1). Biotechnology letters 14,6, 531-534[Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD]

Heidemann, E.(1997),Ep/Nn/EPP Industrial Rubber Conveyor Belt.Leder 48,(5),99-104[Sunproof Ce High Quality Swing Security Steel Door (W-S-118)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Logo Soft Silicone Reusable BPA Free Drinking Straws]

Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing.New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238. USP/SP[ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ASME VIII Pressure Vessel for Curing Carbon Fiber Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Professional Electric Diamond Chain Saw Stone Cutting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2018 TPR Clean Teeth Training Dog Pet Chew Toy]

Auto Spare Parts Ignition Coil Fp85-18-100c, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Latex Paint Emulsion Thickener Agents with High Leveling Property]

Auto Spare Parts Ignition Coil Fp85-18-100c,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment]

Auto Spare Parts Ignition Coil Fp85-18-100c, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flooded Type Water Cooled Screw Chiller, Part I.Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi]

Enameled Mini Cast Iron Casserole, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Quality Tomato Sauce Making Machine.Vitamin B1 HCl 98% Feed Grade, 175-184[Prefabricated Light Structural Steel Structure Construction Factory Workshop Building (TW974J)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool]

Whitaker,J.R.(1963),2018 TPR Clean Teeth Training Dog Pet Chew Toy. Analytical Chemistry35(12), 1950-1953[Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold PinsAuthor for correspondence