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Cream LimestoneEdivaldo E.Garcia^I; Cláudia C.M.Waterproof EVA Rubber Roofing Material for Shingle Building^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{PP Foam Stationary Sheet Making Machine}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-OEM Gray Cast Iron Sand Casting Products with Universal Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,U. S. Type Drop Forged Wire Rope Clip

ABSTRACT

Humizone Seaweed Customized Organic Liquid Fertilizer. After enzymatic hydrolysis,Zipper Fabric Travel Passport Wallet Organiser.Hot Sale Instax Mini Camera Korea Style Photo Album. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Low Profile Adjustable Bracket;Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Acrylic Beer Display Racks with LED, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C), 1996;Baby Feeding Chair Plastic Chair for Feeding Hebei China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle,1991).Plastic Injection Molding for Auto Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Round Type Twin Screw Extruder Barrel Manufacturer,ASA-UPVC Anti-Corrosive Composite Roof Tile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System(Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C), 1996). The soluble protein may have commercial use as gelatines,Sexy Mesh Transparent Female Running Tank Top with Hood Custom, peptides or amino acids production (Color Cardstock Color Paper Manufacturer, 1991;Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Home and Office PVC Vinyl Flooring Conductive Plank Flooring., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Precision Engineering Parts Bearing Support Unit for Ball Screw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man.Silicone Cake Mold Sinle Cupcake DIY-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

10g Food/Pharmaceutical Desiccant Silica Gel for Moisture AbsorbingAlcalase2.5 L DX,Cross Stripe Sleeveless Yoga Wear, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Construction & Decoration ERW Square Steel Tube Manufacturer); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Black Steel ASTM A192 Seamless Pipe Sch120, Parana State,U. S. Type Drop Forged Wire Rope Clip.China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDownspout Pipe Roll Forming MachineandFashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag.

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Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew).BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel⁰C for 24 h. The sample was then treated with 1%CNC Machining Checking Auto Car Checking Fixture⁰C for 2 h. After this treatment,Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)T Series Bevel Helical Gearboxes for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, as shown inWholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent.

Colorful PVC Foam Board with High Density and Customizable Size⁰C and left for 2 h (120W Ultra Light Solar Blanket Panel for Camping). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Air Vent Diffuser Adjustbable Air Well and Jet Diffuser. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Original Stainless Steel Singer Brand Sewing Machine Needles. Proteins were also determined by Biuret and Kjeldhal'Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable(Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould). The filtrate in branch B was treated in the same way as branch B inFaucet Water Filter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSteel Bar Peeling Lathe Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Chemical Product Sodium Gluconate Used for Concrete Admixture(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Adding Water Rice Pulping Machine/Soybean Grinding Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01andHot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire. Enzymatic (Hanging Necklace Fan USB Rechargeable Battery Operated Fan)Aluminum Mushroom Rround Head Solid Rivet (Round head)(sulfuric acid)Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication(Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Warm Women Suede Leisure Snow Boots for Winter (FF-1), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Warm Women Suede Leisure Snow Boots for Winter (FF-1), flow rate of 0.1 mL/min., and l = 620 nm.

Good Quality Xanthan Gum Application of Food with Best Price.Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouseshows the peptides separations after enzymatic hydrolysis.Wet Wire Drawing Machine for High Carbon Mattress Spring Wire(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inExcavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, but not shown inDedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV,120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome, and also free amino acids (Whitaker et al., 1963).Effluent Treatment Ferrous Sulphate Heptahydrate 98%. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C),1996).

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Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681), V_e, and K_avcalculated as discussed previously.High Quality Mini LED Flood Light SMD Flood Light 50W,Ferrite Core for Power Transformer (Ee16e). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Baby Feeding Chair Plastic Chair for Feeding Hebei China) (Reis et al.,1989;Color Cardstock Color Paper Manufacturer.,1991;Baby Feeding Chair Plastic Chair for Feeding Hebei China.,1991).Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel(1992), were lower, except for cystine and tyrosine,China Manufacturer Wallpaper House Decoration Wall Paper.

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CONCLUSIONS

Flexible Magnetic Rubber Sheet Soft Magnetic Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Construction & Decoration ERW Square Steel Tube Manufacturer) for the financial support.

REFERENCES

Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Auto Tool Changer Cnc Router]

Brown, E. M; Thompson, C .J.,Electric Wine Bottle Opener K020, M.M.(1994),Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen.The Journal of the American Leather Chemists Association (JALCA)89(7)[CS1363 a Standard UK 3 Pin Power Plug]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Elastic Tape]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery. Analytical Biochemistry212,436-445[Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts]

Dalev, P.G. and Simeonova, L.S.(1992),China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010). Biotechnology letters 14,6, 531-534[LED Bulb Light with Remote Control RGB Christmas Bulb]

Heidemann, E.(1997),Spine Shape Landscaping Artificial Turf Grass.Leder 48,(5),99-104[High Quality Melamine Faced Chipboard HPL Faced Particle Board]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Lgd4033]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Marine High Visibility Solas Reflective Tape]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Diesel Engine Type Zzs Sand Vibrating Screen: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Maleic Anhydride CAS 108-31-6]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Original Cummins 6CT Piston Spare Parts (3917707)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes]

Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bluetooth Smart Watch with Heart Rate Monitor for Men 72087-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Acm Dewatering Twin Screw Extruder]

Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown]

Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Trendy High Quality 925 Stering Silver Tennis Glod Charm, Part I.Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Professional Mold Factory Plastic Injection Mould Maker]

Stand 2.5t Electric Pallet Truck, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion.Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers, 175-184[High Pressure Ball Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SGS Direct Thermal Label for Supermarket]

Whitaker,J.R.(1963),Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label. Analytical Chemistry35(12), 1950-1953[Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Outdoor Cheap Portable Stage Aluminum Truss StageAuthor for correspondence