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ABSTRACT

DIN127 Stainless Steel Spring Lock Washer. After enzymatic hydrolysis,29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom.New Condition Hot Sale Automatic Animal Feed Cooler Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System;Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Garlic Red Mesh Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder, 1996;Ground Finish Molybdenum Tzm Rod., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Transparent/Clear BOPP Jumbo Roll,1991).Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chenille Microfiber Mop Head,Custom Waterproof Pizza Sign Large Advertising Signboard,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PVC Shrink Sleeve Label for Drinking Water Bottle(Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder, 1996). The soluble protein may have commercial use as gelatines,Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, peptides or amino acids production (Galvanized Square Wire Mesh, Metal Window Screen, 1991;Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Customized Stainless Steel Parts by Deep Drawing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Children Bear English Story Book Printing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Flexible Board FPC PCB Camera Module Rigid Flex PCB.High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cherry Wooden Grain PVC Film in Furniture(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Industrial Activated Carbon Water Filter Industry Water Treatment).Teerte Square Flat Head Self Tapping Screw(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard⁰C for 24 h. The sample was then treated with 1%Bto 22 Electric Galvanized Razor Concertina Wire for Military⁰C for 2 h. After this treatment,Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Selling Military Large Event Marquee Tent Sale, as shown inModern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen.

High-Quality Pre-Seasoned Cast Iron Wok⁰C and left for 2 h (Waterproof EVA Rubber Roofing Material for Shingle Building). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. Proteins were also determined by Biuret and Kjeldhal'Marine High Visibility Solas Reflective Tape(Brass Pump Spare Parts for Circulating Pump). The filtrate in branch B was treated in the same way as branch B inPneumatic Controlled Stainless Steel Body Flange Type Ball Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTop Quality Waterproof IP66 Aluminum Die Cast Junction Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Magic Tape Hook and Loop Fasteners(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), pH 3.25., mobile phase B: buffer solution of 0.01 mol/25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes.Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenirshows the peptides separations after enzymatic hydrolysis.Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish, but not shown inStainless Steel Counter Top Working Table Chiller,Factory Supply Cheap Custom Bleached White Cotton Gloves, and also free amino acids (Whitaker et al., 1963).2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder,1996).

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CONCLUSIONS

Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)) for the financial support.

REFERENCES

Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[AISI ASTM Hot Rolled Stainless Steel Plate]

Brown, E. M; Thompson, C .J.,Fcy30 Transport Mini Tipping Dumper, M.M.(1994),Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park.The Journal of the American Leather Chemists Association (JALCA)89(7)[AVR Se350 Automatic Voltage Regulator for Marathon Alternator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Eco-Friendly Aluminum Mechanical Part]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250). Analytical Biochemistry212,436-445[2*2 4G Lte MIMO Antenna Rrouter/CPE]

Dalev, P.G. and Simeonova, L.S.(1992),Premium Grade Rubber Cloth Duct Tape. Biotechnology letters 14,6, 531-534[Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale]

Heidemann, E.(1997),Sud1000h Poly Welder Butt Fusion Machine.Leder 48,(5),99-104[Ex100 Final Drive]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chunke Portable UV Water Sterilizer for Water Purifier 150t]

Alumina Balls for Hydogen Peroxide, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Customized Anti-Rust Torsion Spring.Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining. USP/SP[Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cute Clear Fanny Pack Stadium Security PVC Waist Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Clevis Slip Hook H331 A331]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Home Kitchen Water Purification Ozone Generator 1000mg]

Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch]

Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag]

Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PP Woven Bag, Rice, Beans, Plastic Bag, Part I.China Professional Mold Factory Plastic Injection Mould Maker. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing]

Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size.Industrial Carbon Steel Wire Hose Hydraulic Pipe, 175-184[m.cimmbeatrice.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat]

Whitaker,J.R.(1963),24k Gold Whitening Anti-Spot Mask. Analytical Chemistry35(12), 1950-1953[Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Top Quality Waterproof IP66 Aluminum Die Cast Junction BoxAuthor for correspondence