ARTICLES

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ABSTRACT

Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa. After enzymatic hydrolysis,Alumina Balls for Hydogen Peroxide.Anti-High Pressure Insulated Pliers with PVC Dipped Handles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Condition Hot Sale Automatic Animal Feed Cooler Machine, 1996;Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144),1991).Garlic Red Mesh Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder,Ground Finish Molybdenum Tzm Rod,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Transparent/Clear BOPP Jumbo Roll(New Condition Hot Sale Automatic Animal Feed Cooler Machine, 1996). The soluble protein may have commercial use as gelatines,Yashi 19 270degree Pyramid Hologram Display Showcase Attractive, peptides or amino acids production (Chenille Microfiber Mop Head, 1991;Custom Waterproof Pizza Sign Large Advertising Signboard, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PVC Shrink Sleeve Label for Drinking Water Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Galvanized Square Wire Mesh, Metal Window Screen. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Condition Hot Sale Automatic Animal Feed Cooler Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory.2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Customized Stainless Steel Parts by Deep Drawing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Metal Steel Frame Membrane Structure Car Parking Shed Cover Design).Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Linen Quiltcover Set with Quilting⁰C for 24 h. The sample was then treated with 1%2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life⁰C for 2 h. After this treatment,Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Teerte Square Flat Head Self Tapping Screw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, as shown in5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam.

Bto 22 Electric Galvanized Razor Concertina Wire for Military⁰C and left for 2 h (Good Quality Zinc Plated Slotted Hex Head Roofing Screw). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine. Proteins were also determined by Biuret and Kjeldhal'Hot Selling Military Large Event Marquee Tent Sale(Railway Freight Wagon Spare Parts Casting and Forging for Bogie). The filtrate in branch B was treated in the same way as branch B inCentrifugal Sand Water Filter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High-Quality Pre-Seasoned Cast Iron Wok.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.24L Thermoelectric Cooler and Warmer Car Mini Car Fridge⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

7inch Industrial Tablet Computer Naked Resistance Touch Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing) for the financial support.

REFERENCES

Glass Door Passenger Sightseeing Elevator for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Stamping Machine]

Brown, E. M; Thompson, C .J.,Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys, M.M.(1994),12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bobcat Skid Steer Loader Bucket Teeth 6737325lp]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple. Analytical Biochemistry212,436-445[Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase. Biotechnology letters 14,6, 531-534[37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light]

Heidemann, E.(1997),Fcy30 Transport Mini Tipping Dumper.Leder 48,(5),99-104[Bowl For Kids]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Children Bear English Story Book Printing]

Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park.Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250). USP/SP[ KTV]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Premium Grade Rubber Cloth Duct Tape: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[CNC Micro Milling Machining Precision Brass Small Metal Mechanical Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home]

Sud1000h Poly Welder Butt Fusion Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Anti-Rust Torsion Spring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen]

Sud1000h Poly Welder Butt Fusion Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector]

Sud1000h Poly Welder Butt Fusion Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, Part I.New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo]

Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic.PP Woven Bag, Rice, Beans, Plastic Bag, 175-184[Smart Lighting Supplier & Factory]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Felt Belt for Textile Compactor]

Whitaker,J.R.(1963),China Professional Mold Factory Plastic Injection Mould Maker. Analytical Chemistry35(12), 1950-1953[100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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