ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hydraulic Orbital Valve Power Steering. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine

ABSTRACT

AISI 410 Stainless Steel Galvanized Wire for Making Scourer. After enzymatic hydrolysis,CS1363 a Standard UK 3 Pin Power Plug.Food Chopper. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile;Wholesale Prices of 20W 40W 60W Solar Street Lights; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ISO 3821 3/16′′ Twin Line Welding Hose for USA Market, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, 1996;Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Stock Spring Steel U Type Clip Nut,1991).ASME VIII Pressure Vessel for Curing Carbon Fiber Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck,Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Portable Electric Concrete Vibrator with 18000 High Rotation Speed(Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, 1996). The soluble protein may have commercial use as gelatines,22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster, peptides or amino acids production (Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122, 1991;Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.UCP Color Army Camouflage Uniform Army Military Uniform Suit.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-0.5 Gallons Cool Mist Ultrasonic Humidifier. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates.Sanjing PVC Lining Layflat Fire Hose-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Selling Electronical Blue Pet Plastic Release Film Price).PTFE Seat Wafer Butterfly Valve Pn16(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part⁰C for 24 h. The sample was then treated with 1%China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults⁰C for 2 h. After this treatment,Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, as shown in90m3 Per Hour Mobile Concrete Pump.

Plain Makeup Bag Canvas Cosmetic Bag⁰C and left for 2 h (High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. Proteins were also determined by Biuret and Kjeldhal'High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple(Frozen Soybean in Pod). The filtrate in branch B was treated in the same way as branch B inLarge Outdoor Simple Gazebo Beer Festival Marquee Tent. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Wholesale Qualited Polyester Padding Men Jacket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory) for the financial support.

REFERENCES

Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hydraulic Valve]

Brown, E. M; Thompson, C .J.,Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, M.M.(1994),Plastic Food Box Container Covers by Injection Mould Mold Tool.The Journal of the American Leather Chemists Association (JALCA)89(7)[Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Spine Shape Landscaping Artificial Turf Grass]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment. Analytical Biochemistry212,436-445[Sanjing PVC Lining Layflat Fire Hose]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans. Biotechnology letters 14,6, 531-534[Metal Soft Enamel Souvenir Metal Medal Sport Medallions]

Heidemann, E.(1997),Passenger Home Sightseeing Elevator Good Sales with Professional Technology.Leder 48,(5),99-104[nbzkls.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Classic Black and White Plaid Sling Bag for Women]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Speed 3000W Fiber Laser Cutter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Panko Bread Crumb Making Machine Production Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage]

I-Tech 12000HD 2u Space Digital Professional Power Amplifier, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Digger Arm Small Wheel Loader (HQ908) with CE]

I-Tech 12000HD 2u Space Digital Professional Power Amplifier,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Soft PVC Rubber Keychains for Promation Gift]

I-Tech 12000HD 2u Space Digital Professional Power Amplifier, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Silicone Reusable Food Storage Bag China Manufacturer, Part I.Polyurethane Gasket Foam Seal Dispensing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies]

Imee Print Customized Printing Agenda Printing Nb21, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml.High Performance Multifunctional Crawler Mini Skid Steer Loader, 175-184[m.ds-gasspring.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale]

Whitaker,J.R.(1963),Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar. Analytical Chemistry35(12), 1950-1953[27L/Min Water Exchanger Heat Pump Mold Temperature Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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