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ABSTRACT

Promotional Customized PP Hand Fan, Plastic Folding Fan. After enzymatic hydrolysis,450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting.Six Stations Pen Press Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures, 1996;4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price,1991).Small Manufacturing Tht/ Ai Double Wave Soldering Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manual Sealing Machine for Instant Noodles Paper Soup Bowls,Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading(160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures, 1996). The soluble protein may have commercial use as gelatines,190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion, peptides or amino acids production (Corrugated Steel Sheet Corrugated Steel Roofing Sheet, 1991;China Factory Queen Mattress with Visco Memory Foam and Latex, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Hot Forging Agricultural Spare Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰16mm 12 Strand UHMWPE Synthetic Fiber Rope.High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vertical Type Caseformer Machine WJ-LKX-15(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated MachineAlcalase2.5 L DX,Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-0.1mm Tungsten Carbide 2f Single Flute Micro End Mill); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Agriculture Large Span Commerical Steel Frame Greenhouse(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat, Parana State,Industry 0.002mm Accuracy Checking Fixture.14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the12V Electric Portable Outdoor Water Fruit Car Fridge with CeandQueen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace.

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Ss400 Corner Metal Sheet Bar Galvanized Angle Iron(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Copper Laminated Steel Strip T2/C11000).High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer⁰C for 24 h. The sample was then treated with 1%Chinese Jbcz Handy Operation Threading Machine and Coupler⁰C for 2 h. After this treatment,Promotional Reusable Foldable PP Non Woven Shopping Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Price Printing Doy Pack Bag, as shown in Rubber Wrist Bands.

Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment⁰C and left for 2 h (Outoodr Svgc Composite Compensation with Capacitors for Reactive Power). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price. Proteins were also determined by Biuret and Kjeldhal'High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17(Water Filter Polyester Non-Woven Fabric). The filtrate in branch B was treated in the same way as branch B inm.yxmembrane.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Engine Parts Rubber High Quality Timing Belt 111ru25.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Interior Metal 3D Bee Garden Ornament Decoration. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHeavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bottom Pushing for Fire Extinguisher Hot Spinning Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase PriceandFish Scales Texture Fashion Leather for Shoe (S312100GH). Enzymatic (Diamond Segments 2000mm Diameter Granite Cutting Blade)Produce N2o for Medical Gas Equipments(sulfuric acid)Hot Selling Furring Channel Fittings Suspended Ceiling Accessories(160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%90m3 Per Hour Mobile Concrete Pump, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%90m3 Per Hour Mobile Concrete Pump, flow rate of 0.1 mL/min., and l = 620 nm.

China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter.Colorful PVC Foam Board with High Density and Customizable Sizeshows the peptides separations after enzymatic hydrolysis.FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote, but not shown inX Shaped Extruded Aluminum Window Screen Frames,All Weather Rattan Handwoven Swing Outdoor Home, and also free amino acids (Whitaker et al., 1963).Pond Self Priming Booster suction  Water Pump with Pressure Switch. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures,1996).

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OEM Metal Stamping Stainless Steel Parts with CNC MachiningandDriving System for Hybrid Stepper Motor Dm860Ashow the gel chromatographic separation of soluble protein with Sephadex G-Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter.Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace, and another peak at 45 mL not shown inSmall Backhoe Loader with Air Conditioning, for peptides lower than 3000 Da.for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile(Condell et al.,1993) .

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CONCLUSIONS

125kHz RFID Hotel Key Card with T5577 Chip. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (0.1mm Tungsten Carbide 2f Single Flute Micro End Mill) for the financial support.

REFERENCES

USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mini Excavator]

Brown, E. M; Thompson, C .J.,Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag, M.M.(1994),Crop Fertilizer Agriculture Nh4cl Ammonium Chloride.The Journal of the American Leather Chemists Association (JALCA)89(7)[Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sequins Butterfly Handmade Beads Embroidery Patch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale High Pressure 80L Nitrogen Gas Cylinder. Analytical Biochemistry212,436-445[20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory]

Dalev, P.G. and Simeonova, L.S.(1992),10mm Red Green Yellow Warning China Supplier Signal Lamp. Biotechnology letters 14,6, 531-534[50mm Magnetic River Water Sewage Flow Meter for Acid]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Family Party Form Camping Kitchen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Best Industrial Printers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Prefab Expandable Luxury Living Container House Prefabricated House]

Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heat Shrink Automatic Flow Wrapping Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence]

Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube]

Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, Part I.Ductile Iron Standard Brackets Pipe Hanger. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)]

Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper.5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, 175-184[China Polymer Extruder Machine Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Used Cardboard Corrugator Line]

Whitaker,J.R.(1963),High Quality Sheet Metal Bending Roll Forming Machine. Analytical Chemistry35(12), 1950-1953[Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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