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ABSTRACT

3PC Stainless Steel Flanged End Ball Valve 3 Inches 150. After enzymatic hydrolysis,Ysdcnc Manual Sheet Metal Plate Bending Roller Machine.Betaine Anhydrous 98% Food Grade Good Quality Competitive Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover;Bearing Stand Type Cam Follower; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Electric LED light Aroma Diffuser, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner, 1996;DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps,1991).7PCS RGBW LED Plastic PAR Light Wall Washer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm,Kindergarten Playground Preschool Kids Plastic Slide and Swing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks(Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner, 1996). The soluble protein may have commercial use as gelatines,Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200, peptides or amino acids production (Railway Freight Wagon Spare Parts Casting and Forging for Bogie, 1991;Rainsky Ceiling Mounted Stainless Steel Shower Heads, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Sizes Acrylic Shoes Storage Display Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Casting Cover Aluminum Die Casting Products.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser.SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole, Parana State,PVC Cross Joint Pipe Fittings Equal Pipe Cross.Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for TruckandPearls Flake 99% Factory Naoh Caustic Soda.

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20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)).Hot Sale Laser Projector RGB Laser Light for Stage(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net⁰C for 24 h. The sample was then treated with 1%Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer⁰C for 2 h. After this treatment,Acm Dewatering Twin Screw Extruder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, as shown inParts for Crane Outrigger Hydraulic Oil Cylinder.

Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple⁰C and left for 2 h (Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Good Quality Good Price China Plastic Injection Molding Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pet Hair Remover Glove for Dog, Grooming Gloves Brush. Proteins were also determined by Biuret and Kjeldhal'Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa(Custom Car Seat Belt Buckle Mold Maker). The filtrate in branch B was treated in the same way as branch B inCircular Saw. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Alumina Balls for Hydogen Peroxide.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Anti-High Pressure Insulated Pliers with PVC Dipped Handles. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Selling Electronical Blue Pet Plastic Release Film Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Condition Hot Sale Automatic Animal Feed Cooler Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Yashi 19 270degree Pyramid Hologram Display Showcase Attractive.Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlityshows the peptides separations after enzymatic hydrolysis.Chenille Microfiber Mop Head(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAuto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, but not shown inFood Grade Stainless Steel Threated Butterfly Valve,Custom Waterproof Pizza Sign Large Advertising Signboard, and also free amino acids (Whitaker et al., 1963).PVC Shrink Sleeve Label for Drinking Water Bottle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner,1996).

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CONCLUSIONS

Cherry Wooden Grain PVC Film in Furniture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine) for the financial support.

REFERENCES

Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Attractive Gondola Setup Commercial Island Refrigerator Commercial Multideck Round Refrigerator]

Brown, E. M; Thompson, C .J.,Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV, M.M.(1994),Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44).The Journal of the American Leather Chemists Association (JALCA)89(7)[Medical Supply U40 Disposable Insulin Syringe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Manhole Galvanized Steel Trench Cover for Drain]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump. Analytical Biochemistry212,436-445[German Technology Automatic Stacking System in Red Brick Automatic Production Line]

Dalev, P.G. and Simeonova, L.S.(1992),Linen Quiltcover Set with Quilting. Biotechnology letters 14,6, 531-534[Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[All Weather Rattan Handwoven Swing Outdoor Home]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Tablet Case for Samsung Tab a 10.1 2019]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)]

Bto 22 Electric Galvanized Razor Concertina Wire for Military, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Wholesale Qualited Polyester Padding Men Jacket]

Bto 22 Electric Galvanized Razor Concertina Wire for Military,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft]

Bto 22 Electric Galvanized Razor Concertina Wire for Military, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, Part I.Hot Selling Military Large Event Marquee Tent Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading]

High-Quality Pre-Seasoned Cast Iron Wok, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle.Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine, 175-184[Food Paper Bag Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Clay Tiles Making Machine with Latest Technology]

Whitaker,J.R.(1963),Marine High Visibility Solas Reflective Tape. Analytical Chemistry35(12), 1950-1953[I-Tech 12000HD 2u Space Digital Professional Power Amplifier]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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