ARTICLES

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ABSTRACT

304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment. After enzymatic hydrolysis,Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans.Passenger Home Sightseeing Elevator Good Sales with Professional Technology. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Vacuum Stainless Steel Casting Products;Chicken Farming Materials Poultry Battery Cages Layer Battery Cage; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (I-Tech 12000HD 2u Space Digital Professional Power Amplifier, 1996;Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Silicone Reusable Food Storage Bag China Manufacturer,1991).Polyurethane Gasket Foam Seal Dispensing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Imee Print Customized Printing Agenda Printing Nb21,Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Performance Multifunctional Crawler Mini Skid Steer Loader(I-Tech 12000HD 2u Space Digital Professional Power Amplifier, 1996). The soluble protein may have commercial use as gelatines,Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, peptides or amino acids production (Chunke Portable UV Water Sterilizer for Water Purifier 150t, 1991;Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Xjm Series Rubber Extruding Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stop Dog From Jumping or Climbing Your Chain Link Fence.. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (I-Tech 12000HD 2u Space Digital Professional Power Amplifier, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰SGS Direct Thermal Label for Supermarket.Water Filter Polyester Non-Woven Fabric-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industry Pet Recycling Systems, Industry Pet Recycling Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Valve Bag Dry Mortar Bagging Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Bearing Stand Type Cam Follower).High Quality 500m 100lb Super Strong Braided Fishing Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China⁰C for 24 h. The sample was then treated with 1%37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light⁰C for 2 h. After this treatment,Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, as shown inVacuum Squeeze Filter Press for Coal Flotation Tailings.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom All Kinds of Acrylic Shoe Display Racks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInsect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Medical Supply U40 Disposable Insulin Syringe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Water Chiller Air Cooled Chiller Industrial Chiller for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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4 Styles Blue Glass Crystal Butt Beads Spiral Plug, V_e, and K_avcalculated as discussed previously.Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve,Industrial Activated Carbon Water Filter Industry Water Treatment. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (0.1mm Tungsten Carbide 2f Single Flute Micro End Mill) (Reis et al.,1989;Chunke Portable UV Water Sterilizer for Water Purifier 150t.,1991;Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer.,1991).2016 New Generation Sausage Making Machine(1992), were lower, except for cystine and tyrosine,Magnetic Mixer Mixing Tank with Bottom Agitator (MTK).

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CONCLUSIONS

Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer) for the financial support.

REFERENCES

New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Korea Lens 42lb TV Backlight Use for LG Innotek Drt 3.0 42′′ a]

Brown, E. M; Thompson, C .J.,China Supplier Building Construction Steel Frame (ZY431), M.M.(1994),iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Polyester Coated Stainless Steel Ladder Type Metal Ties]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Lab Equipment portable Fume Hood. Analytical Biochemistry212,436-445[China Excavator Radiator /Oil Radiator /Water Radiator Manufacture]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt. Biotechnology letters 14,6, 531-534[Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday]

Heidemann, E.(1997),10ton Telescopic Hydraulic Truck Crane.Leder 48,(5),99-104[Aluminum Frame for Screen Printing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[380mm Working Area Concrete Diamond Floor Edge Grinder]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PRO-Installer Fiber Optic Termination Kit Lk-6016]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cracker Multi Rows Trayless Packaging Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PSF 800 Flat Plate Upper Discharge Centrifuge]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flat Surface Mounted 300mm 24W LED Panel Light for Interior]

Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2 Quick Change Flexible Roloc Disc-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Warm Women Suede Leisure Snow Boots for Winter (FF-1)]

Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price]

Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack, Part I.High Quality Aluminium Windows Thermal Break China Supplier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line]

Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01.PRO-Installer Fiber Optic Termination Kit Lk-6016, 175-184[Guitar Side Bending Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor]

Whitaker,J.R.(1963),Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace. Analytical Chemistry35(12), 1950-1953[ Head Lamp Fabia Mining Lamp]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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