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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light. After enzymatic hydrolysis,Stainless Steel Counter Top Working Table Chiller.Cashew Grading Machine for Cashew Processing Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;4X4 Wd Car Tail Light for Ranger 2012-2016;Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera, 1996;Hot Sale Power Press Perforated Metal Punch Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (100%Polyester Satin Fabric for Blouse,1991).Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools,High-End Compression Rash Guards, Long Sleeve Compression Shirt,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo(Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera, 1996). The soluble protein may have commercial use as gelatines,Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office, peptides or amino acids production (Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters, 1991;9~12 Inch Mechanical Backing Plate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;DIN929 Low-Cost Supply Hexagon Weld Nut., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Efficiency Solar Cell Photovoltaic 300W Hot Sell. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 1060m3/H Lamella Clarifier for River Water Treatment.SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Super Fireproof Antibiosis Household Spc Vinyl Floor Plank(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

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Heavy Duty Wood Door Calibrating Wide Belt Sander(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (7PCS RGBW LED Plastic PAR Light Wall Washer).Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK0C for 24 h. The sample was then treated with 1%X-Humate 95% Min Super Sodium Humate (Nut Moradant)0C for 2 h. After this treatment,Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Women Summer Swimsuit Chest Zip Bathing Suit's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hemp Seed Oil Body Lotion Moisturizes The Skin, as shown inChicken Layer Cage Poultry Cage Chicken Cages.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavCustom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Galvanized Hexagonal Wire Mesh Gabion / Gabion Box0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Must 4kw 2HP Pump Power Inverter Ags Bts Available, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate.Promotional Reusable Foldable PP Non Woven Shopping Bagshows the peptides separations after enzymatic hydrolysis.Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPVC Cross Joint Pipe Fittings Equal Pipe Cross, but not shown inRechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries,Barbed Wire Right off The Border Wall to Protect Homes, and also free amino acids (Whitaker et al., 1963).Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera,1996).

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5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist) for the financial support.


Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Direct Optical Intensity Modulation Optical Transmitter with 4 Individual Outputs]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector. Analytical Biochemistry212,436-445[Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Air Vent Diffuser Adjustbable Air Well and Jet Diffuser]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor Type 40kgs Movable Ice Maker for Store Use]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flexible Steel Band]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[XL-21 Type OEM Motive Electrical Switchgear Power Cabinet]

Whitaker,J.R.(1963),Common Rail Diesel Injector Nozzle Dlla153p977. Analytical Chemistry35(12), 1950-1953[Hairdressing Equipment Takara Belmont Barber Chair High Quality]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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