ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults

ABSTRACT

Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China. After enzymatic hydrolysis,Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01).Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plain Makeup Bag Canvas Cosmetic Bag;14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple, 1996;Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Wholesale Qualited Polyester Padding Men Jacket,1991).5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer,Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition(High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple, 1996). The soluble protein may have commercial use as gelatines,Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, peptides or amino acids production (River Water Cleaning Sludge Sediment Machine Lamella Clarifier, 1991;Polyester Coated Stainless Steel Ladder Type Metal Ties, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Copper Laminated Steel Strip T2/C11000. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Halal Empty Gelatin Capsule for Medicine.Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Gym Fitness Exercise Sporting Body Building Bicep Press(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift TruckAlcalase2.5 L DX,Metal Steel Frame Membrane Structure Car Parking Shed Cover Design, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light).1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine⁰C for 24 h. The sample was then treated with 1%Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver⁰C for 2 h. After this treatment,Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plastic Food Box Container Covers by Injection Mould Mold Tool, as shown inChina Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010).

304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment⁰C and left for 2 h (Hot Sale Ultra Slim Elevator Digital Signage). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Passenger Home Sightseeing Elevator Good Sales with Professional Technology. Proteins were also determined by Biuret and Kjeldhal'Vacuum Stainless Steel Casting Products(Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034). The filtrate in branch B was treated in the same way as branch B inWhite Cardboard Take Away Take out Meal Box Cutting Machine 1214. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Chicken Farming Materials Poultry Battery Cages Layer Battery Cage.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avI-Tech 12000HD 2u Space Digital Professional Power Amplifier(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Silicone Reusable Food Storage Bag China Manufacturer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Polyurethane Gasket Foam Seal Dispensing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Imee Print Customized Printing Agenda Printing Nb21, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CNC Fiber Laser Cutting Machine for Sheet and TubeandAluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml. Enzymatic (High Performance Multifunctional Crawler Mini Skid Steer Loader)Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar(sulfuric acid)Chunke Portable UV Water Sterilizer for Water Purifier 150t(High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress, flow rate of 0.1 mL/min., and l = 620 nm.

Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain.Small Manufacturing Tht/ Ai Double Wave Soldering Machineshows the peptides separations after enzymatic hydrolysis.Xjm Series Rubber Extruding Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inKwikstage Scaffolding System-Diagonal Brace, but not shown inChenille Microfiber Mop Head,Stop Dog From Jumping or Climbing Your Chain Link Fence., and also free amino acids (Whitaker et al., 1963).SGS Direct Thermal Label for Supermarket. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple,1996).

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ADSS Newest Ultra Therapy Skin Tightening 3D Hifuand Common Rail Injector Testershow the gel chromatographic separation of soluble protein with Sephadex G-Water Filter Polyester Non-Woven Fabric.Industry Pet Recycling Systems, Industry Pet Recycling Line, and another peak at 45 mL not shown inPop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, for peptides lower than 3000 Da.Sublimation Polyester Sports Tshirt for Adult(Condell et al.,1993) .

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Heavy Duty Marquee Advertising Pop up Tent, V_e, and K_avcalculated as discussed previously.Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer,Fire Extinguisher UV Curing Machine Cylinder Drying Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY) (Reis et al.,1989;River Water Cleaning Sludge Sediment Machine Lamella Clarifier.,1991;Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry.,1991).Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set(1992), were lower, except for cystine and tyrosine,Energy-Saving Biogas Double Stove for Sale.

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CONCLUSIONS

Valve Bag Dry Mortar Bagging Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size) for the financial support.

REFERENCES

High Quality 500m 100lb Super Strong Braided Fishing Line, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.yxzx-extrusion.com]

Brown, E. M; Thompson, C .J.,20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China, M.M.(1994),37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Rigid PCB Manufacturer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags. Analytical Biochemistry212,436-445[Electrosurgical Generator High Frequency Electricity Kinfe]

Dalev, P.G. and Simeonova, L.S.(1992),PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint. Biotechnology letters 14,6, 531-534[Zipper Fabric Travel Passport Wallet Organiser]

Heidemann, E.(1997),Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine.Leder 48,(5),99-104[Urine Analysis Rapid Test Kit URS-10T 11MA 12MA Test]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Wholesale Hot Sale Rubber Silicone Seal Diaphragm]

PTFE Seat Wafer Butterfly Valve Pn16, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending.Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch. USP/SP[Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Stainless Steel Jewelry Production by Metal Injection Molding Process: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Minarelli Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial Carbon Steel Wire Hose Hydraulic Pipe]

Factory Directly Selling Engine Standard Spark Plug, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Logo Silicone Wristband, Rubber Wrist Bands-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould]

Factory Directly Selling Engine Standard Spark Plug,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale]

Factory Directly Selling Engine Standard Spark Plug, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom All Kinds of Acrylic Shoe Display Racks, Part I.Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls]

Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Medical Supply U40 Disposable Insulin Syringe.Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, 175-184[www.jxmelamine.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Price Diamond Wire Mesh Chain Link Fence]

Whitaker,J.R.(1963),Water Chiller Air Cooled Chiller Industrial Chiller for Sale. Analytical Chemistry35(12), 1950-1953[Underground Garden 6W High Power Outdoor LED Lawn Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mango Drying Machine/Solar Fruit Drying MachineAuthor for correspondence