ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High-Efficient Htk Walnut Shell Filter for Water Purification. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)

ABSTRACT

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100). After enzymatic hydrolysis,Popular European Style White Marble Coffee Tables for Dinnig Room.High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System;Kwikstage Scaffolding System-Diagonal Brace; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.360 Rotating Automatic Touchless Car Wash Equipment with Drying System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, 1996;Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump,1991).Sell Rubber / EVA / PE/PVC Foam Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sodium Gluconate 98% Min Industry Grade for Chemical Admixture,Brake Disc,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign(48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, 1996). The soluble protein may have commercial use as gelatines,24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat, peptides or amino acids production (MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote, 1991;Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;LED Screen Display Wireless Charging 10000mAh Power Bank., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.25kg 50kg Plastic PP Woven Feed Bags.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-360 Degree Rotation Medical Seat Cushion. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom LCD Screen Video Box for Package.Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

2019 Panfeng Color Painting Three Flavor Soft Ice Cream MachineAlcalase2.5 L DX,Alu 2319 Slimline 12mm Wide Recessed Profile, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Custom Size Christmas Gift Personalized Watch Boxes for Men(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope, Parana State,Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576).8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the1.5/2 power 50Hz ZN SERIES Vibrator motorandWaterproof Night Vision USA License Plate Frame Rearview Reverse Camera.

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Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Toast Stamp Loaf Bread Tin Pan (ZMA)).New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)⁰C for 24 h. The sample was then treated with 1%Stainless Steel Cross Recesed Flat Head Self Tapping Screw⁰C for 2 h. After this treatment,Our Perforated Aluminum & Solid Panel for New Office Wall Cladding. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Kids Trucker Hat, Kids Mesh Cap, as shown inBig Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen.

Wd190V Handheld Full Body Scanner Hand Held Metal Detector⁰C and left for 2 h (Baby Feeding Chair Plastic Chair for Feeding Hebei China). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hydraulic Orbital Valve Power Steering. Proteins were also determined by Biuret and Kjeldhal'W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine(Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust). The filtrate in branch B was treated in the same way as branch B inHigh Speed Double Colors 2 Layers PVC Layflat Hose Extrusion Machine Line Online Yarn Fiber Braiding Knitting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,AISI 410 Stainless Steel Galvanized Wire for Making Scourer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CS1363 a Standard UK 3 Pin Power Plug. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFood Chopper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Prices of 20W 40W 60W Solar Street Lights⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ISO 3821 3/16′′ Twin Line Welding Hose for USA Market, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Mineral Industry Magnet Ore Ball Milling MachineandBeauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond. Enzymatic (Factory Stock Spring Steel U Type Clip Nut)ASME VIII Pressure Vessel for Curing Carbon Fiber Parts(sulfuric acid)Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck(48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut, flow rate of 0.1 mL/min., and l = 620 nm.

Portable Electric Concrete Vibrator with 18000 High Rotation Speed.CS1363 a Standard UK 3 Pin Power Plugshows the peptides separations after enzymatic hydrolysis.22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Elastic Tape, but not shown inFactory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts,Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122, and also free amino acids (Whitaker et al., 1963).Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner,1996).

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Lgd4033andBluetooth Smart Watch with Heart Rate Monitor for Men 72087show the gel chromatographic separation of soluble protein with Sephadex G-Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing.UCP Color Army Camouflage Uniform Army Military Uniform Suit, and another peak at 45 mL not shown inMarine High Visibility Solas Reflective Tape, for peptides lower than 3000 Da.0.5 Gallons Cool Mist Ultrasonic Humidifier(Condell et al.,1993) .

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Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates, V_e, and K_avcalculated as discussed previously.Sanjing PVC Lining Layflat Fire Hose,Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes) (Reis et al.,1989;MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote.,1991;Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox.,1991).China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light(1992), were lower, except for cystine and tyrosine,Automatic CNC Glass Cutting Machine Glass Cutting Table.

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CONCLUSIONS

Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo) for the financial support.

REFERENCES

Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM Supply Battery Charger 7 Stage Charging - Wall Mounted Voltage Regulator WDA-5000VA - Ligao]

Brown, E. M; Thompson, C .J.,Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price, M.M.(1994),Pet PP Rope and Broom Wire Drawing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Professional Mold Factory Plastic Injection Mould Maker]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[SGS Direct Thermal Label for Supermarket]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup. Analytical Biochemistry212,436-445[Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds]

Dalev, P.G. and Simeonova, L.S.(1992),PTFE Seat Wafer Butterfly Valve Pn16. Biotechnology letters 14,6, 531-534[Outdoor Cheap Portable Stage Aluminum Truss Stage]

Heidemann, E.(1997),Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part.Leder 48,(5),99-104[Industrial Infrared Burners]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine]

20 Bottles Electric Wine Display Cooler, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults.Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China. USP/SP[PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Silicone Rubber Electronic Cable with High Flexibility]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Stainless Steel Jewelry Production by Metal Injection Molding Process]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Prefabricated Container Houses]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[6.6kw LiFePO4 Charger for Lithium Battery Pack]

Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plain Makeup Bag Canvas Cosmetic Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Folding Aluminium Easy Move Choral Stage with Wheels for School]

Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage]

Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, Part I.1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Superior Watertightness JS Polymer Cement Waterproof Coating for Basement]

High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry.Factory Wholesale Qualited Polyester Padding Men Jacket, 175-184[Hair Trimmer Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel]

Whitaker,J.R.(1963),5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam. Analytical Chemistry35(12), 1950-1953[Silicone Cake Mold Sinle Cupcake DIY]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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