ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Factor Direct Adult Sex Toy Doll for Male Sex. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L

ABSTRACT

Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory. After enzymatic hydrolysis,WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W.Worm Gear Fluorine Lined Butterfly Valve Flanged Type. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera;Poultry House, Livestock, Poultry Farm; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2, 1996;Black Slim PU Leather Passport Travel Wallet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe,1991).Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3 Colors Tactical Shooting Hunting Camouflage Clothes Suit,1.3KG Fire breaker for kitchen,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign(Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2, 1996). The soluble protein may have commercial use as gelatines,Korean Integrated CTO Activated Carbon Filter for Water Treatment, peptides or amino acids production (Vacuum Squeeze Filter Press for Coal Flotation Tailings, 1991;Modular Portable Shipping Container Lkit Building Home Villa House, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Supplier of Excavator Spare Parts for Caterpillar Cat330.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow.Wheel-Type-Carriage Precision Heavy Duty Linear Guide-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Double-Offset Double Flanged Stainless Steel Butterfly Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle, Parana State,C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L.Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMini Shampoo Plastic End Caps Cosmetic Packaging Screw Capsand20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.

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OEM Logo Soft Silicone Reusable BPA Free Drinking Straws(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor).Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4 Drawer Steel Office Vertical Filing Cabinet⁰C for 24 h. The sample was then treated with 1%12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car⁰C for 2 h. After this treatment,Parts for Crane Outrigger Hydraulic Oil Cylinder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Boned Polyester Woven Faux Suede Sofa Fabric's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making, as shown inKitchen Metal Dish Storage Rack (JP-SC984C).

20 Bottles Electric Wine Display Cooler⁰C and left for 2 h ( Aerospace Equipment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High-Efficient Htk Walnut Shell Filter for Water Purification. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576). Proteins were also determined by Biuret and Kjeldhal'Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)(Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray). The filtrate in branch B was treated in the same way as branch B inHigh Current Capacitor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Popular European Style White Marble Coffee Tables for Dinnig Room.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Kwikstage Scaffolding System-Diagonal Brace(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.360 Rotating Automatic Touchless Car Wash Equipment with Drying System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

BS1387 Class B 2 Inch Sch40 Gi Pipe GalvanizedandIN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump. Enzymatic (Sell Rubber / EVA / PE/PVC Foam Board)Sodium Gluconate 98% Min Industry Grade for Chemical Admixture(sulfuric acid)Brake Disc(Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign, flow rate of 0.1 mL/min., and l = 620 nm.

24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat.Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Conditionshows the peptides separations after enzymatic hydrolysis.MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in LED Bayomet Vehicle Lamps, but not shown inPhillip Countersunk Head Screw Hot DIP Galvanized,Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder, and also free amino acids (Whitaker et al., 1963).LED Screen Display Wireless Charging 10000mAh Power Bank. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2,1996).

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Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt, V_e, and K_avcalculated as discussed previously.Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning,2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap) (Reis et al.,1989;Vacuum Squeeze Filter Press for Coal Flotation Tailings.,1991;Black Slim PU Leather Passport Travel Wallet.,1991).Alu 2319 Slimline 12mm Wide Recessed Profile(1992), were lower, except for cystine and tyrosine,High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo.

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CONCLUSIONS

Custom Size Christmas Gift Personalized Watch Boxes for Men. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars) for the financial support.

REFERENCES

12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.jumpamusement.com]

Brown, E. M; Thompson, C .J.,8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector, M.M.(1994),Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hydraulic Orbital Valve Power Steering]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115. Analytical Biochemistry212,436-445[Customized Large Ss Stamping/ Bending Sheet Metal Parts]

Dalev, P.G. and Simeonova, L.S.(1992),China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX). Biotechnology letters 14,6, 531-534[Hanging Necklace Fan USB Rechargeable Battery Operated Fan]

Heidemann, E.(1997),Stainless Steel Cross Recesed Flat Head Self Tapping Screw.Leder 48,(5),99-104[Heat Resistance Clear Polished Customized Fused Quartz Glass Disc]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chicken Farming Materials Poultry Battery Cages Layer Battery Cage]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kids Trucker Hat, Kids Mesh Cap: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Color Coated Steel Strip]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Electric LED light Aroma Diffuser]

Wd190V Handheld Full Body Scanner Hand Held Metal Detector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest]

Wd190V Handheld Full Body Scanner Hand Held Metal Detector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer]

Wd190V Handheld Full Body Scanner Hand Held Metal Detector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hydraulic Orbital Valve Power Steering, Part I.W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill]

AISI 410 Stainless Steel Galvanized Wire for Making Scourer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CS1363 a Standard UK 3 Pin Power Plug.Food Chopper, 175-184[Bathroom Accessory Small Door Handles]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Auto Spare Parts 12629037 Ignition Coil for Cadillac]

Whitaker,J.R.(1963),Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile. Analytical Chemistry35(12), 1950-1953[Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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