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ABSTRACT

Best Selling Household Living Room Mat Chenille Carpets. After enzymatic hydrolysis,130HP 4WD Large Farm Power Engine Tractor From High Quality Factory.Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Large Size Outdoor Store Front Signs Advertising Billboard;Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1325 CNC Engraving Router, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, 1996;T6s 86 PCD Core Bit for Geotechnical Drilling., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines,1991).CNC Machining 6601 Aluminum Parts with Anodized Automoblile. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cummins L10 Engine Piston Cooling Nozzle 3070218,Round Shape All Color Pets Feeding Dog Bowl of China Supplies,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wvrb Series Planetary Gearbox Used for Robot(Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, 1996). The soluble protein may have commercial use as gelatines,NPK 15 15 15 Compound Fertilizer at a Hot Selling, peptides or amino acids production (Mini Blown Film Extrusion, 1991;Pearls Flake 99% Factory Naoh Caustic Soda, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Auto Spare Parts 12629037 Ignition Coil for Cadillac., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality PVC Resin with Low Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Small Backhoe Loader with Air Conditioning. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Line Scan Uvss System Portable Under Vehicle Surveillance.Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:FSTN Type Monochrome Graphic Display Cog LCD with Backlight(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Building Material High Quality 316 Stainless Steel Grating(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Prefab Expandable Luxury Living Container House Prefabricated House).Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sc-2400 Automatic Multi Function Sosn Side Drilling Machine⁰C for 24 h. The sample was then treated with 1%Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair⁰C for 2 h. After this treatment,Quality Shaper Machine Bc6085 Shapping Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Thermoplastic Road Marking Paint Glass Beads's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, as shown inGalvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence.

Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts⁰C and left for 2 h (Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5. Proteins were also determined by Biuret and Kjeldhal'Free Sample! AC Power Contactor for Sale(Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)). The filtrate in branch B was treated in the same way as branch B in20kw Low Temp Industrial Cw 5000 Water Oil Chiller for Induction Heating. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PA System Loudspeaker 100W Outdoor Column Speaker .

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBreathable Surgical Disposable 3 Ply Earloop Face Mask(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flower Design Custom Made Silk Scarf, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ladder Type Cable Tray with Ce/ GOST/ TUV/UL.Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chairshows the peptides separations after enzymatic hydrolysis.12V Electric Portable Outdoor Water Fruit Car Fridge with Ce(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBest Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, but not shown inCow Dung Chicken Manure Organic Fertilizer Making Machine,Fine Bubble Diffuser for Air Purifier, and also free amino acids (Whitaker et al., 1963).Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel,1996).

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CONCLUSIONS

Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer) for the financial support.

REFERENCES

High Quality & High Speed Straight Line Wire Drawing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Removable Insulation Cover High Temperature Insulation Silicone Coated Fiberglass Cloth]

Brown, E. M; Thompson, C .J.,High Speed 3000W Fiber Laser Cutter, M.M.(1994),Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Sheet Metal Stamping Bending Product Manufacturing Service]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Water Chiller Air Cooled Chiller Industrial Chiller for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting. Analytical Biochemistry212,436-445[Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)]

Dalev, P.G. and Simeonova, L.S.(1992),Personality Cat Eye Sunglasses. Biotechnology letters 14,6, 531-534[13.56 MHz Fudan FM11RF08 Chip Card]

Heidemann, E.(1997),ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline.Leder 48,(5),99-104[Household Cleaning Cloth Microfiber Classic Multipurpose Clean Cloth 4001]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken]

Travelling Weekender Mens Canvas Duffle Gym Sports Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),UV Coated PVC Panel for Interior Wall Board.Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.. USP/SP[Colombia Outdoor P5 Pantallas LED Display]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Food Package Aluminum Foil Tray Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5W Ultra-Thin Tiltable LED Down Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Naipu Horizontal Ash Slurry Pump Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electrophoresis Assembly Infilltration Tank for Effluent Treatment]

Wholesale 30W LED Down Lights with Budget Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Embossed Textured Wallpaper for Solvent Printing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PA System Loudspeaker 100W Outdoor Column Speaker ]

Wholesale 30W LED Down Lights with Budget Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple]

Wholesale 30W LED Down Lights with Budget Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray, Part I.4-14 Manual Staple Gun with Staples for Hand Tools. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Soosan Excavator Hydraulic Hammer for Construction Demolition]

500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Smallest Portable Waste Transformer Oil Purifier Machine Zy-6.Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles, 175-184[Steel And Tube Stainless]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[130HP 4WD Large Farm Power Engine Tractor From High Quality Factory]

Whitaker,J.R.(1963),High Speed Automatic Packing Machine (GK-650 BA). Analytical Chemistry35(12), 1950-1953[Viton O Ring Kit with Excellent with Oil Resistance]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Metal Steel Frame Membrane Structure Car Parking Shed Cover DesignAuthor for correspondence