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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment

ARTICLES


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Pizza Insulated BagEdivaldo E.GarciaI; Cláudia C.M.Plastic Injection for OEM ProductI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiGlass Lined Reactor for Chemical Factory


IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event


ABSTRACT

Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen. After enzymatic hydrolysis,Activated Carbon Strong Effect Peeling off Face Mask Mud.Type Price Prefabricated Roof Tile in Sudan. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion;Factory Supply 100% Natural Epimedium Extract for Male Health; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug, 1996;Top10 1080P H. 264 P2p CCTV WiFi IP Camera., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture,1991).Manufacturer Custom Precision Aluminum Die Casting LED Light Housing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate,1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Color Child Book Printing Service(Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug, 1996). The soluble protein may have commercial use as gelatines,1.5 Inch High Pressure Petrol/Gasoline Water Pump, peptides or amino acids production (Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine, 1991;Equal and Unequal High Quality Hot Rolled Steel Angle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Halal Chicken Slaughter Machine for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8.3 PCS Headband for Baby Girls with Hair Bows-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

High Quality Xanthan Gum for Personal Care and Cosmetic ApplicationsAlcalase2.5 L DX,Manual Vertical Car Key Cutting Machine for Locksmith, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Transparent Color Stretch Wrap Film Manual Operate Wrap Film, Parana State,Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event.Good Price Welding Electrode (e7018/e7018-1), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2D LED Rope Lighting Motif Lights for Christmas DecorationandQuality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter.

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Ep/Nn/EPP Industrial Rubber Conveyor Belt(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing).Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-2380C for 24 h. The sample was then treated with 1%Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies0C for 2 h. After this treatment,Auto Spare Parts Ignition Coil Fp85-18-100c. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Flooded Type Water Cooled Screw Chiller, as shown inJIS S Type Cast Iron Water Filter Dn25.

Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday0C and left for 2 h (100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Enameled Mini Cast Iron Casserole. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Good Quality Tomato Sauce Making Machine. Proteins were also determined by Biuret and Kjeldhal'Vitamin B1 HCl 98% Feed Grade(High Strength Rigid PVC Plastic Foam Board for FRP). The filtrate in branch B was treated in the same way as branch B inF-DMD Electrical Insulation Material (6641). The final soluble protein shown were used for peptides separations using gel permeation chromatography,2018 TPR Clean Teeth Training Dog Pet Chew Toy.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Good Quality Zinc Plated Slotted Hex Head Roofing Screw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavVertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Orthopedic Fiberglass Splint, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Amazon Hot Selling Kids Playing Pop up Goal, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light BlinkerandSmall Frame Hydraulic Press Machine for Bending Price. Enzymatic (3.5-7.0g Ozone Generator Purification ---Commercial)Baby Feeding Chair Plastic Chair(sulfuric acid)Reflective Hand Hold Thermoplastic Road Marking(Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04), flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04), flow rate of 0.1 mL/min., and l = 620 nm.

Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge.ASME/ASTM Butt-Welding Stainless Steel Plate Flangeshows the peptides separations after enzymatic hydrolysis.Food Grade Stainless Steel Threated Butterfly Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTransport System China Manufacturer for Table Belt Conveyor, but not shown inHh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover,PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine, and also free amino acids (Whitaker et al., 1963).Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug,1996).

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Fcy30 Transport Mini Tipping DumperandStationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Tableshow the gel chromatographic separation of soluble protein with Sephadex G-Suction Function Handheld Cool and Heat Food Bowl for Baby.9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white), and another peak at 45 mL not shown inThreaded Stainless Steel Motorized Electric Floating 3PC Ball Valve, for peptides lower than 3000 Da.JIS S Type Cast Iron Water Filter Dn25(Condell et al.,1993) .

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Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut, Ve, and Kavcalculated as discussed previously.Factor Direct Adult Sex Toy Doll for Male Sex,C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine) (Reis et al.,1989;Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine.,1991;Top10 1080P H. 264 P2p CCTV WiFi IP Camera.,1991).Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory(1992), were lower, except for cystine and tyrosine,WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W.

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CONCLUSIONS

Worm Gear Fluorine Lined Butterfly Valve Flanged Type. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape) for the financial support.

REFERENCES

H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Welded Landing Craft Aluminium Fishing Boats for Sale]

Brown, E. M; Thompson, C .J.,Poultry House, Livestock, Poultry Farm, M.M.(1994),1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[20 Bottles Electric Wine Display Cooler]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Price Gi/PPGI Roofing Sheet in China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2. Analytical Biochemistry212,436-445[ Cheap]

Dalev, P.G. and Simeonova, L.S.(1992),Black Slim PU Leather Passport Travel Wallet. Biotechnology letters 14,6, 531-534[China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)]

Heidemann, E.(1997),ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe.Leder 48,(5),99-104[m.hrbpackgroup.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch]

Plastic Injection for OEM Product, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce.3 Colors Tactical Shooting Hunting Camouflage Clothes Suit. USP/SP[Q32 Series Tumble Belt/Crawler Shot Blasting Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plain Makeup Bag Canvas Cosmetic Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1.3KG Fire breaker for kitchen: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[SJIII-S300 Automatic Tomato Sauce Ketchup Chili Shampoo Filling Packing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′)]

Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Korean Integrated CTO Activated Carbon Filter for Water Treatment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)]

Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China]

Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Vacuum Squeeze Filter Press for Coal Flotation Tailings, Part I.Modular Portable Shipping Container Lkit Building Home Villa House. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Orthopedic Fiberglass Splint]

New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Supplier of Excavator Spare Parts for Caterpillar Cat330.Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal, 175-184[Video]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plain Chipboard]

Whitaker,J.R.(1963),Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow. Analytical Chemistry35(12), 1950-1953[CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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