ARTICLES

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ABSTRACT

120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome. After enzymatic hydrolysis,Effluent Treatment Ferrous Sulphate Heptahydrate 98%.Rechargeable 9V Li Ion Battery with Micro USB Charging Port. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Transport System China Manufacturer for Table Belt Conveyor;Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Mini LED Flood Light SMD Flood Light 50W, 1996;Ferrite Core for Power Transformer (Ee16e)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel,1991).China Manufacturer Wallpaper House Decoration Wall Paper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Flexible Magnetic Rubber Sheet Soft Magnetic Tape,Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electric Wine Bottle Opener K020(High Quality Mini LED Flood Light SMD Flood Light 50W, 1996). The soluble protein may have commercial use as gelatines,Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen, peptides or amino acids production (China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery, 1991;China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Spine Shape Landscaping Artificial Turf Grass., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Mini LED Flood Light SMD Flood Light 50W, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Diesel Engine Type Zzs Sand Vibrating Screen.Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bluetooth Smart Watch with Heart Rate Monitor for Men 72087(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Men Fashion Plaid Design Polyester Ties (WH24)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Design Indoor Artificial Mini Rockery Fountains).70m Series Aluminiun Plastic Coated Profile for Widnows(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Arrival Wooden Kids Cleaning Set Toy for Education W10d251⁰C for 24 h. The sample was then treated with 1%Vertical Type Automatic Box Erector Machine⁰C for 2 h. After this treatment,Air Cooled Scroll Type Water Chiller for Industrial Process. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, as shown inGalvanized Square Washer with Timber Constructions.

Rotary Vibrating Sieve Machine for Compost⁰C and left for 2 h (Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine. Proteins were also determined by Biuret and Kjeldhal'Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa(Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China). The filtrate in branch B was treated in the same way as branch B inwww.well-pcb.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCondoms/Sex Toys/Capsule Toy Vending Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Type 40kgs Movable Ice Maker for Store Use, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Prefab Building Insulated Interior Concrete Partition Walls. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stand 2.5t Electric Pallet Truck) for the financial support.

REFERENCES

Disk Filter System with Automatic Backwash Valves, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electric Fireplace Insert For Existing Fireplace High Quality Supplier]

Brown, E. M; Thompson, C .J.,Eco-Friendly Recycling PS Foam Container Production Line, M.M.(1994),Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[DIN Standard Gratings/Gavlanized Steel Grating]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using. Analytical Biochemistry212,436-445[En1092-1 05 Pn16 Blind Flat Face Flange]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing. Biotechnology letters 14,6, 531-534[10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing]

Heidemann, E.(1997),Jacquard Football Fans Acrylic Sports Warmer Scarf.Leder 48,(5),99-104[Medical Oxygen Concentrator Pressure Swing Adsorption]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Hot Forging Agricultural Spare Parts]

Warm Women Suede Leisure Snow Boots for Winter (FF-1), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound.Small Blinking Battery Operated Luminary Plastic LED Tea Lights. USP/SP[Ladder Type Cable Tray with Ce/ GOST/ TUV/UL]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Injection Service Prototype Manufacture Custom Plastic Molding]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker]

Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ysdcnc Manual Sheet Metal Plate Bending Roller Machine]

Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light]

Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Precision Plastic Mold Manufacturer in China, Part I.Mango Drying Machine/Solar Fruit Drying Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube]

Mineral Industry Magnet Ore Ball Milling Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut.Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel, 175-184[Wholesale Amber Cosmetic Packaging Glass Jar for Face Cream]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dark Green Plastic PVC Film for Christmas Tree Leaves]

Whitaker,J.R.(1963),Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump. Analytical Chemistry35(12), 1950-1953[Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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