ARTICLES

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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ms Gi Pipe Dn15~Dn400 Gi Pipe List, 1996;Low-E Insulating Glass Curtain Wall., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024,1991).Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Design Underwear for Men Cotton Mens Boxer Briefs,Nylon Balance Bike with Water Transfer Printing Malaysia,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Anti Slip Rubber Mat(Ms Gi Pipe Dn15~Dn400 Gi Pipe List, 1996). The soluble protein may have commercial use as gelatines,5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap, peptides or amino acids production (Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor, 1991;Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automotive Perforated Metal Stamping Part for Furniture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ms Gi Pipe Dn15~Dn400 Gi Pipe List, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Car Accessories Seat for Child.Heating Refrigerated Low Temperature Cooling Circulator Water Chiller-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Zipper Pouch Pistachio Nuts Premade Packing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2010 Used Foton Truck Mixer, Parana State,High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame.Single Board 3.7V~24VDC Microwave Detector PCB, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ms Gi Pipe Dn15~Dn400 Gi Pipe List, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAir Cooled Scroll Type Water Chiller for Industrial ProcessandMini Blown Film Extrusion.

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Automatic Chicken Nipple Drinker System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer).Industrial Hydraulic Ice Grape Press Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Common Rail Diesel Injector Nozzle Dlla153p977⁰C for 24 h. The sample was then treated with 1%Betaine 98% Betaina Feed Grade⁰C for 2 h. After this treatment,Glass Salt & Pepper Mill/Grinder Glass Spice Jar. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outsole Trimming Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel, as shown inRectangular Box Facial Tissue Paper Fk-110.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Auto Two Piece Folder Gluer for Corrugated Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Industry 0.002mm Accuracy Checking Fixture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Colombia Outdoor P5 Pantallas LED Display) for the financial support.

REFERENCES

Promotional Customized PP Hand Fan, Plastic Folding Fan, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[42 Stepper Motor Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting, M.M.(1994),Six Stations Pen Press Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Strength Rigid PVC Plastic Foam Board for FRP]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine. Analytical Biochemistry212,436-445[LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker]

Dalev, P.G. and Simeonova, L.S.(1992),Vertical Automatic Granule Bag Packing Machine. Biotechnology letters 14,6, 531-534[ASME/ASTM Butt-Welding Stainless Steel Plate Flange]

Heidemann, E.(1997),Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil.Leder 48,(5),99-104[Nbr Reclaimed Rubber]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Transport System China Manufacturer for Table Belt Conveyor]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Water Treatment Anthracite]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fcy30 Transport Mini Tipping Dumper]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Molding Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table]

Small Manufacturing Tht/ Ai Double Wave Soldering Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manual Sealing Machine for Instant Noodles Paper Soup Bowls-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve]

Small Manufacturing Tht/ Ai Double Wave Soldering Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems]

Small Manufacturing Tht/ Ai Double Wave Soldering Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck, Part I.Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine]

190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Corrugated Steel Sheet Corrugated Steel Roofing Sheet.China Factory Queen Mattress with Visco Memory Foam and Latex, 175-184[China OEM Custom Automotive Rubber Spare Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women]

Whitaker,J.R.(1963),High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing. Analytical Chemistry35(12), 1950-1953[Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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