ARTICLES

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^IICNPq/RHAE/biotechnology-Round Casting Iron Trench Cover Manhole Cover Frame. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane

ABSTRACT

Eco-Friendly Aluminum Mechanical Part, CNC Machining. After enzymatic hydrolysis,Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench.Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ductile Iron Flanged Swing Check Valve, 1996;Ozonated Water for Vegetables and Fruits Washing Ozone Generator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose,1991).Day and Night Zebra Blind Fabric Z1602. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock,Automatic OPP Slitter Cutting Machine (HX-320FQ vertical),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Impact Sound Isolation Wall Clip(Ductile Iron Flanged Swing Check Valve, 1996). The soluble protein may have commercial use as gelatines,Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving, peptides or amino acids production (German Technology Automatic Stacking System in Red Brick Automatic Production Line, 1991;Top Quality Galvanized Iron Wire as Binding Wire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;13.56 MHz Fudan FM11RF08 Chip Card., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ductile Iron Flanged Swing Check Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine.100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Square Premium Glass Perfume Bottle 100ml(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cast Iron Butterfly Valve with Handles(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine).Furniture Grade E1 Glue Plywood with Competitive Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer⁰C for 24 h. The sample was then treated with 1%Underground Garden 6W High Power Outdoor LED Lawn Light⁰C for 2 h. After this treatment,Stamping Metal Parts Manufacturer Sheet Parts Fabrication. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, as shown inCustom Car Seat Belt Buckle Mold Maker.

Hexagonal Netting/ Gabion Box/ Gabion Basket⁰C and left for 2 h (Baby Feeding Chair Plastic Chair). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Different Thickness High Quality Aluminum Foil for Glass Bottle Lid. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla. Proteins were also determined by Biuret and Kjeldhal'Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages(Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity). The filtrate in branch B was treated in the same way as branch B inFibras De Molde. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Ozone Disinfection Machine Ozone Generator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PVC Profile with Net Making Machine Used for Window Sealing Profile⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bobby Flay Websites Restaurant Martha Stewart Designer Tray, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1325 CNC Engraving Router, V_e, and K_avcalculated as discussed previously.Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel,T6s 86 PCD Core Bit for Geotechnical Drilling. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Efficiency Mini-Pleat HEPA Filter (H13/H14)) (Reis et al.,1989;German Technology Automatic Stacking System in Red Brick Automatic Production Line.,1991;Ozonated Water for Vegetables and Fruits Washing Ozone Generator.,1991).Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines(1992), were lower, except for cystine and tyrosine,CNC Machining 6601 Aluminum Parts with Anodized Automoblile.

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CONCLUSIONS

Cummins L10 Engine Piston Cooling Nozzle 3070218. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep) for the financial support.

REFERENCES

Round Shape All Color Pets Feeding Dog Bowl of China Supplies, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Medical X-ray Film (Blue Sensitive)]

Brown, E. M; Thompson, C .J.,Wvrb Series Planetary Gearbox Used for Robot, M.M.(1994),NPK 15 15 15 Compound Fertilizer at a Hot Selling.The Journal of the American Leather Chemists Association (JALCA)89(7)[German Technology Automatic Stacking System in Red Brick Automatic Production Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mini Blown Film Extrusion. Analytical Biochemistry212,436-445[Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Pearls Flake 99% Factory Naoh Caustic Soda. Biotechnology letters 14,6, 531-534[Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board]

Heidemann, E.(1997),Auto Spare Parts 12629037 Ignition Coil for Cadillac.Leder 48,(5),99-104[1325 Wood Cutting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Line Scan Uvss System Portable Under Vehicle Surveillance: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Wholesale Qualited Polyester Padding Men Jacket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flex Rigid PCB Printted Circuit Board Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft]

Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),FSTN Type Monochrome Graphic Display Cog LCD with Backlight-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading]

Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Clay Tiles Making Machine with Latest Technology]

Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3D Embossed Iron on Label Clothing Heat Transfer Label, Part I.Glass Food Jar & Plastic Lid. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[I-Tech 12000HD 2u Space Digital Professional Power Amplifier]

0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Easy Operation Low Investment Cassava Silo.CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, 175-184[Lighting G45 Globe Shape E14 LED Lamps Bulb]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PTFE Seat Wafer Butterfly Valve Pn16]

Whitaker,J.R.(1963),100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve. Analytical Chemistry35(12), 1950-1953[Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Selling Military Large Event Marquee Tent SaleAuthor for correspondence