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ABSTRACT

Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet. After enzymatic hydrolysis,1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price.Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Food Box Container Covers by Injection Mould Mold Tool, 1996;304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans,1991).Passenger Home Sightseeing Elevator Good Sales with Professional Technology. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vacuum Stainless Steel Casting Products,Chicken Farming Materials Poultry Battery Cages Layer Battery Cage,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer(Plastic Food Box Container Covers by Injection Mould Mold Tool, 1996). The soluble protein may have commercial use as gelatines,I-Tech 12000HD 2u Space Digital Professional Power Amplifier, peptides or amino acids production (Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer, 1991;High Quality Silicone Reusable Food Storage Bag China Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Polyurethane Gasket Foam Seal Dispensing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Imee Print Customized Printing Agenda Printing Nb21.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Food Box Container Covers by Injection Mould Mold Tool, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Performance Multifunctional Crawler Mini Skid Steer Loader.Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Chunke Portable UV Water Sterilizer for Water Purifier 150t(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

SGS Direct Thermal Label for Supermarket, Parana State,Polyester Dust Sport Gym Fabric Shoes Drawstring Bag.Water Filter Polyester Non-Woven Fabric, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Food Box Container Covers by Injection Mould Mold Tool, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theInside Angle Profile Corner Bracket for 30A Series Aluminum Profileand12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car.

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Industry Pet Recycling Systems, Industry Pet Recycling Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai).Sublimation Polyester Sports Tshirt for Adult(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heavy Duty Marquee Advertising Pop up Tent⁰C for 24 h. The sample was then treated with 1%Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer⁰C for 2 h. After this treatment,Fire Extinguisher UV Curing Machine Cylinder Drying Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Energy-Saving Biogas Double Stove for Sale, as shown inAuto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale.

Valve Bag Dry Mortar Bagging Machine⁰C and left for 2 h (4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality 500m 100lb Super Strong Braided Fishing Line. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China. Proteins were also determined by Biuret and Kjeldhal'37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light(Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China). The filtrate in branch B was treated in the same way as branch B inGrey Iron Casting And Machining. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFull Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Directly Selling Engine Standard Spark Plug, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Xjm Series Rubber Extruding Machine) for the financial support.

REFERENCES

High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[100000 Hours Lifetime 200W High Lumen LED Street Light with Chips]

Brown, E. M; Thompson, C .J.,4 Styles Blue Glass Crystal Butt Beads Spiral Plug, M.M.(1994),Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[PVC Textile Fabric for Outdoor Sports Waterproof Backpack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial Activated Carbon Water Filter Industry Water Treatment. Analytical Biochemistry212,436-445[Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),2016 New Generation Sausage Making Machine. Biotechnology letters 14,6, 531-534[Nbm High Quality Auto Part Factory Price 6pk2310]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Garlic Red Mesh Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Precision Tungsten Carbide Machine Customized Cutting Tool]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Supplier Building Construction Steel Frame (ZY431): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Turbocharger Kit]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ FRP Profiles]

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iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ MUG]

iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, Part I.10ton Telescopic Hydraulic Truck Crane. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bto 22 Electric Galvanized Razor Concertina Wire for Military]

Whitaker,J.R.(1963),Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China. Analytical Chemistry35(12), 1950-1953[New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Speed Automatic Packing Machine (GK-650 BA)Author for correspondence