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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Rectangular Box Facial Tissue Paper Fk-110. After enzymatic hydrolysis,SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price.Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;AAC Block Production Line / AAC Equipment Manufacturing/ Machinery;CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Q32 Series Tumble Belt/Crawler Shot Blasting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Galvanized Bar Grating, 1996;High Quality Custom Self Adhesive Leather Patch for Jackets., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale,1991).380mm Working Area Concrete Diamond Floor Edge Grinder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam,New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Discussion and Interpretation System(Galvanized Bar Grating, 1996). The soluble protein may have commercial use as gelatines,7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, peptides or amino acids production (Frozen Soybean in Pod, 1991;Custom Sheet Metal Stamping Bending Product Manufacturing Service, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof EVA Rubber Roofing Material for Shingle Building., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Gray Cast Iron Sand Casting Products with Universal Parts.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-U. S. Type Drop Forged Wire Rope Clip. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Galvanized Bar Grating, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Humizone Seaweed Customized Organic Liquid Fertilizer.Zipper Fabric Travel Passport Wallet Organiser-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale Instax Mini Camera Korea Style Photo Album(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Baby Feeding Chair Plastic Chair for Feeding Hebei China, Parana State,Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment.Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Galvanized Bar Grating, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory Supply 100% Natural Epimedium Extract for Male HealthandInsulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool.

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Plastic Injection Molding for Auto Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (T6 Outdoor Waterproof Tactical Flashlight).Round Type Twin Screw Extruder Barrel Manufacturer(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ASA-UPVC Anti-Corrosive Composite Roof Tile0C for 24 h. The sample was then treated with 1%Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System0C for 2 h. After this treatment,Sexy Mesh Transparent Female Running Tank Top with Hood Custom. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Color Cardstock Color Paper Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, as shown in24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.

Home and Office PVC Vinyl Flooring Conductive Plank Flooring0C and left for 2 h (Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Precision Engineering Parts Bearing Support Unit for Ball Screw. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection. Proteins were also determined by Biuret and Kjeldhal'165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man(High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157). The filtrate in branch B was treated in the same way as branch B inMicky Mouse Tube Si Type Aluminum Material Si Cylinder Tube. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Silicone Cake Mold Sinle Cupcake DIY.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Construction & Decoration ERW Square Steel Tube Manufacturer0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Black Steel ASTM A192 Seamless Pipe Sch120, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Different Size Glass Test TubesandChina Excavator Radiator /Oil Radiator /Water Radiator Manufacture. Enzymatic (Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine)BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film(sulfuric acid)Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel(Galvanized Bar Grating, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CNC Machining Checking Auto Car Checking Fixture, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CNC Machining Checking Auto Car Checking Fixture, flow rate of 0.1 mL/min., and l = 620 nm.

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Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology AnalyzerandGalvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturershow the gel chromatographic separation of soluble protein with Sephadex G-Air Vent Diffuser Adjustbable Air Well and Jet Diffuser.Original Stainless Steel Singer Brand Sewing Machine Needles, and another peak at 45 mL not shown inNbm High Quality Auto Part Factory Price 6pk2310, for peptides lower than 3000 Da.Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable(Condell et al.,1993) .

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Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance, Ve, and Kavcalculated as discussed previously.Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve,Steel Bar Peeling Lathe Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (OEM Service Machining Milling Turning Service Custom CNC Aluminum Case) (Reis et al.,1989;Frozen Soybean in Pod.,1991;High Quality Custom Self Adhesive Leather Patch for Jackets.,1991).Chemical Product Sodium Gluconate Used for Concrete Admixture(1992), were lower, except for cystine and tyrosine,Adding Water Rice Pulping Machine/Soybean Grinding Machine.

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Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


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Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ethylene Based Vam Supplier Suppliers for Sale]

Brown, E. M; Thompson, C .J.,Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire, M.M.(1994),Hanging Necklace Fan USB Rechargeable Battery Operated Fan.The Journal of the American Leather Chemists Association (JALCA)89(7)[ FRP Profiles]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Mushroom Rround Head Solid Rivet (Round head). Analytical Biochemistry212,436-445[ MUG]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication. Biotechnology letters 14,6, 531-534[Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed]

Heidemann, E.(1997),Warm Women Suede Leisure Snow Boots for Winter (FF-1).Leder 48,(5),99-104[]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Bto 22 Electric Galvanized Razor Concertina Wire for Military]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Speed Automatic Packing Machine (GK-650 BA)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electric Paint Sprayer Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bracelets Bangles Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar]

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Effluent Treatment Ferrous Sulphate Heptahydrate 98%,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker]

Effluent Treatment Ferrous Sulphate Heptahydrate 98%, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Transport System China Manufacturer for Table Belt Conveyor, Part I.Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels]

Whitaker,J.R.(1963),Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel. Analytical Chemistry35(12), 1950-1953[Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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