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ABSTRACT

Transparent/Clear BOPP Jumbo Roll. After enzymatic hydrolysis,Yashi 19 270degree Pyramid Hologram Display Showcase Attractive.Chenille Microfiber Mop Head. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Galvanized Square Wire Mesh, Metal Window Screen, 1996;Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter,1991).OEM Customized Stainless Steel Parts by Deep Drawing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Children Bear English Story Book Printing,Flexible Board FPC PCB Camera Module Rigid Flex PCB,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing(Galvanized Square Wire Mesh, Metal Window Screen, 1996). The soluble protein may have commercial use as gelatines,Cherry Wooden Grain PVC Film in Furniture, peptides or amino acids production (Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, 1991;Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Linen Quiltcover Set with Quilting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Galvanized Square Wire Mesh, Metal Window Screen, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life.Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Teerte Square Flat Head Self Tapping Screw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Selling Military Large Event Marquee Tent Sale, Parana State,Ground Finish Molybdenum Tzm Rod.High-Quality Pre-Seasoned Cast Iron Wok, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Galvanized Square Wire Mesh, Metal Window Screen, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theShima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Conditionand LED Bayomet Vehicle Lamps.

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2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic CNC Glass Cutting Machine Glass Cutting Table).Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Marine High Visibility Solas Reflective Tape⁰C for 24 h. The sample was then treated with 1%24L Thermoelectric Cooler and Warmer Car Mini Car Fridge⁰C for 2 h. After this treatment,Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Top Quality Waterproof IP66 Aluminum Die Cast Junction Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Magic Tape Hook and Loop Fasteners, as shown inTop Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook.

Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool⁰C and left for 2 h (Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor. Proteins were also determined by Biuret and Kjeldhal'Starch Doy Bag Packaging Machine(Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light). The filtrate in branch B was treated in the same way as branch B inClassical Fedora Trilby Women Wool Felt Hat with Leather Buckle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Soft PVC Rubber Keychains for Promation Gift. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avKorean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Supply Cheap Custom Bleached White Cotton Gloves, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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7inch Industrial Tablet Computer Naked Resistance Touch Screen.Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrapshows the peptides separations after enzymatic hydrolysis.Glass Door Passenger Sightseeing Elevator for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFRP Counter Flow Low Noise Round Type Cooling Tower 600rt, but not shown inPipe Conveyor Belt,Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys, and also free amino acids (Whitaker et al., 1963).12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Galvanized Square Wire Mesh, Metal Window Screen,1996).

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CONCLUSIONS

Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights) for the financial support.

REFERENCES

New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Speed Passenger Elevator]

Brown, E. M; Thompson, C .J.,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, M.M.(1994),OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Electric LED light Aroma Diffuser]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PP Woven Bag, Rice, Beans, Plastic Bag. Analytical Biochemistry212,436-445[Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer]

Dalev, P.G. and Simeonova, L.S.(1992),China Professional Mold Factory Plastic Injection Mould Maker. Biotechnology letters 14,6, 531-534[ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill]

Heidemann, E.(1997),Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated.Leder 48,(5),99-104[Side Vertical DIP-16 Pin USB Type C 3.1 Connector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Auto Spare Parts 12629037 Ignition Coil for Cadillac]

Garlic Red Mesh Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size.Industrial Carbon Steel Wire Hose Hydraulic Pipe. USP/SP[Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[St1000 Fire Resistant Steel Cord Rubber Belt]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-24k Gold Whitening Anti-Spot Mask: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2016 New Designs Emboss Pattern Crossbody Bag for Women]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment]

Clay Tiles Making Machine with Latest Technology, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),DIN Standard Gratings/Gavlanized Steel Grating-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket]

Clay Tiles Making Machine with Latest Technology,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Color Coated Aluminum Coils]

Clay Tiles Making Machine with Latest Technology, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:50mm Magnetic River Water Sewage Flow Meter for Acid, Part I.PE Biodegradable Sheet Product Line/Single Screw Extruder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Performance Multifunctional Crawler Mini Skid Steer Loader]

27L/Min Water Exchanger Heat Pump Mold Temperature Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Felt Belt for Textile Compactor.Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, 175-184[48V off-Grid Solar Pure Sine Wave Inverter 3000W 4000W]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts]

Whitaker,J.R.(1963),Metal Soft Enamel Souvenir Metal Medal Sport Medallions. Analytical Chemistry35(12), 1950-1953[Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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