ARTICLES

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ABSTRACT

Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line. After enzymatic hydrolysis,Brass Pump Spare Parts for Circulating Pump.Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lantern for Garden Decoration, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bending Sheet Metal Custom-Made Aluminum Bracket, 1996;New Shacman F3000 6X4 Truck Trailer Head., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump,1991).Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cylindrical Shape Beer Glass Bottle for Wine Storage,Plastic Injection for OEM Product,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024(Bending Sheet Metal Custom-Made Aluminum Bracket, 1996). The soluble protein may have commercial use as gelatines,Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event, peptides or amino acids production (Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen, 1991;Activated Carbon Strong Effect Peeling off Face Mask Mud, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Type Price Prefabricated Roof Tile in Sudan., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Supply 100% Natural Epimedium Extract for Male Health. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bending Sheet Metal Custom-Made Aluminum Bracket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust.Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Top10 1080P H. 264 P2p CCTV WiFi IP Camera(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Full Color Child Book Printing Service, Parana State,7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security.1.5 Inch High Pressure Petrol/Gasoline Water Pump, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Bending Sheet Metal Custom-Made Aluminum Bracket, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theThermoplastic Road Marking Paint Glass BeadsandGalvanized Hexagonal Wire Mesh Gabion / Gabion Box.

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Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding).Equal and Unequal High Quality Hot Rolled Steel Angle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Halal Chicken Slaughter Machine for Sale⁰C for 24 h. The sample was then treated with 1%Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop⁰C for 2 h. After this treatment,2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3 PCS Headband for Baby Girls with Hair Bows, as shown inGarden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Transparent Color Stretch Wrap Film Manual Operate Wrap Film. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGood Price Welding Electrode (e7018/e7018-1)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory Stock Spring Steel U Type Clip NutandAuto Spare Parts Ignition Coil Fp85-18-100c. Enzymatic (vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment)Flooded Type Water Cooled Screw Chiller(sulfuric acid)Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday(Bending Sheet Metal Custom-Made Aluminum Bracket, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Enameled Mini Cast Iron Casserole, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Enameled Mini Cast Iron Casserole, flow rate of 0.1 mL/min., and l = 620 nm.

Good Quality Tomato Sauce Making Machine.Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2shows the peptides separations after enzymatic hydrolysis.Vitamin B1 HCl 98% Feed Grade(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom, but not shown inOutdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight,2018 TPR Clean Teeth Training Dog Pet Chew Toy, and also free amino acids (Whitaker et al., 1963).Good Quality Zinc Plated Slotted Hex Head Roofing Screw. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bending Sheet Metal Custom-Made Aluminum Bracket,1996).

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High Quality Orthopedic Fiberglass Splint, V_e, and K_avcalculated as discussed previously.Amazon Hot Selling Kids Playing Pop up Goal,Small Frame Hydraulic Press Machine for Bending Price. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Price 6mm Stainless Steel Wire Rope 7*7) (Reis et al.,1989;Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen.,1991;New Shacman F3000 6X4 Truck Trailer Head.,1991).3.5-7.0g Ozone Generator Purification ---Commercial(1992), were lower, except for cystine and tyrosine,Baby Feeding Chair Plastic Chair.

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CONCLUSIONS

Reflective Hand Hold Thermoplastic Road Marking. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate) for the financial support.

REFERENCES

Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminium/Aluminum Coil Mill Finish (A1050 1060 1100 3003 3105 5005 5052)]

Brown, E. M; Thompson, C .J.,Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge, M.M.(1994),Food Grade Stainless Steel Threated Butterfly Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Glass Food Jar & Plastic Lid]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[NPK 15 15 15 Compound Fertilizer at a Hot Selling]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine. Analytical Biochemistry212,436-445[Prefab Steel Structure Shed for Storage Warehouse]

Dalev, P.G. and Simeonova, L.S.(1992),Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker. Biotechnology letters 14,6, 531-534[Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine]

Heidemann, E.(1997),Suction Function Handheld Cool and Heat Food Bowl for Baby.Leder 48,(5),99-104[28/410 Drawing Cap Plastic Cap Oil Bottle Lid]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rebonded Foam Machina]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Vitamin B1 HCl 98% Feed Grade]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)]

Factor Direct Adult Sex Toy Doll for Male Sex, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Atf-805 Auto Transmission System Flushing Machine with CE/SGS]

Factor Direct Adult Sex Toy Doll for Male Sex,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tumble Belt Type Shot Blasting Machine for Iron Castings Parts]

Factor Direct Adult Sex Toy Doll for Male Sex, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory, Part I.WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238]

Worm Gear Fluorine Lined Butterfly Valve Flanged Type, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera.Poultry House, Livestock, Poultry Farm, 175-184[Fuel Injector Nozzle Ce6479-S1 for High Quality]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair]

Whitaker,J.R.(1963),1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine. Analytical Chemistry35(12), 1950-1953[Aluminum Low Profile Adjustable Bracket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water PumpAuthor for correspondence