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ABSTRACT

1650mm*80mm Stable Power Laser Tube. After enzymatic hydrolysis,FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit.High Quality Lead Ingot 99.994 Reasonable Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Micro-Combustion Tube, Test Tube (HK-224);Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3W LED Explosion Proof Emergency Exit Light, 1996;Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stationery PP Clipboard With 7 Inch Scale Ruler,1991).Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle,PVC Cross Joint Pipe Fittings Equal Pipe Cross,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3PC Stainless Steel Flanged End Ball Valve 3 Inches 150(3W LED Explosion Proof Emergency Exit Light, 1996). The soluble protein may have commercial use as gelatines,Ysdcnc Manual Sheet Metal Plate Bending Roller Machine, peptides or amino acids production (Betaine Anhydrous 98% Food Grade Good Quality Competitive Price, 1991;2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bearing Stand Type Cam Follower., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Electric LED light Aroma Diffuser.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3W LED Explosion Proof Emergency Exit Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306.Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:7PCS RGBW LED Plastic PAR Light Wall Washer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom Sizes Acrylic Shoes Storage Display Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless).High Quality Casting Cover Aluminum Die Casting Products(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags⁰C for 24 h. The sample was then treated with 1%304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser⁰C for 2 h. After this treatment,SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, as shown inAutomotive Acrylic Wholesale Masking Tape Factory Price.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Laser Projector RGB Laser Light for Stage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Acm Dewatering Twin Screw Extruder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Alumina Balls for Hydogen Peroxide.Fine Bubble Diffuser for Air Purifiershows the peptides separations after enzymatic hydrolysis.Anti-High Pressure Insulated Pliers with PVC Dipped Handles(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inModern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace, but not shown inCommercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine,Hot Selling Electronical Blue Pet Plastic Release Film Price, and also free amino acids (Whitaker et al., 1963).DIN127 Stainless Steel Spring Lock Washer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3W LED Explosion Proof Emergency Exit Light,1996).

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CopperandOEM Customized Stainless Steel Parts by Deep Drawingshow the gel chromatographic separation of soluble protein with Sephadex G-29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom.New Condition Hot Sale Automatic Animal Feed Cooler Machine, and another peak at 45 mL not shown inOne Arm Motorized Multifunction Hospital Equipment for China Supply, for peptides lower than 3000 Da.Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System(Condell et al.,1993) .

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Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144), V_e, and K_avcalculated as discussed previously.Garlic Red Mesh Bag,Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (British Standard 2 Gang 1 Way Wall Light Switch) (Reis et al.,1989;Betaine Anhydrous 98% Food Grade Good Quality Competitive Price.,1991;Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making.,1991).Ground Finish Molybdenum Tzm Rod(1992), were lower, except for cystine and tyrosine,Transparent/Clear BOPP Jumbo Roll.

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CONCLUSIONS

Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks) for the financial support.

REFERENCES

Chenille Microfiber Mop Head, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM Automatic Flow 220L/H Speed Adjustable Water Return Brushless Submersible Water Amphibious Pumps DC 12V]

Brown, E. M; Thompson, C .J.,Custom Waterproof Pizza Sign Large Advertising Signboard, M.M.(1994),PVC Shrink Sleeve Label for Drinking Water Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[1 Inch NPT Screwed Globe Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4. Analytical Biochemistry212,436-445[Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal]

Dalev, P.G. and Simeonova, L.S.(1992),Galvanized Square Wire Mesh, Metal Window Screen. Biotechnology letters 14,6, 531-534[CNC Machining 6601 Aluminum Parts with Anodized Automoblile]

Heidemann, E.(1997),Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory.Leder 48,(5),99-104[Outdoor Flood Light Stadium Sport Court Field Parking Lot 100W LED Flood Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll]

Gen 2+ or 3 Day/Night Vision Scope, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter.OEM Customized Stainless Steel Parts by Deep Drawing. USP/SP[Food Grade Silicone Baby Pacifier for Newborns]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Children Bear English Story Book Printing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Quality Shaper Machine Bc6085 Shapping Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Date Laser Marking Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery]

Flexible Board FPC PCB Camera Module Rigid Flex PCB, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[16mm 12 Strand UHMWPE Synthetic Fiber Rope]

Flexible Board FPC PCB Camera Module Rigid Flex PCB,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ozonated Water for Vegetables and Fruits Washing Ozone Generator]

Flexible Board FPC PCB Camera Module Rigid Flex PCB, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cherry Wooden Grain PVC Film in Furniture, Part I.Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Blue White Yellow Red Black Nylon Mosquito  Window Netting]

Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44).Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump, 175-184[210d Oxford Fabric Indoor Grow Kit]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Concrete Aluminum Formwork System Aluminium Formwork]

Whitaker,J.R.(1963),Linen Quiltcover Set with Quilting. Analytical Chemistry35(12), 1950-1953[Worm Gear Fluorine Lined Butterfly Valve Flanged Type]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery PoleAuthor for correspondence