ARTICLES

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ABSTRACT

Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet. After enzymatic hydrolysis,Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump.ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Auto Two Piece Folder Gluer for Corrugated Box, 1996;British Standard 2 Gang 1 Way Wall Light Switch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts,1991).Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume,Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine(Auto Two Piece Folder Gluer for Corrugated Box, 1996). The soluble protein may have commercial use as gelatines,P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails, peptides or amino acids production (Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire, 1991;Rectangular Stainless Steel Pipe SS304, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.6.6kw LiFePO4 Charger for Lithium Battery Pack.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Auto Two Piece Folder Gluer for Corrugated Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰50m Depth Long Range Metal Detector with Reliable Quality.CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting, Parana State,PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material.Six Stations Pen Press Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Auto Two Piece Folder Gluer for Corrugated Box, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theProfile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting FittingsandBakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags.

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FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut).Vertical Automatic Granule Bag Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil⁰C for 24 h. The sample was then treated with 1%160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures⁰C for 2 h. After this treatment,4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Small Manufacturing Tht/ Ai Double Wave Soldering Machine, as shown inVertical Type Caseformer Machine WJ-LKX-15.

Manual Sealing Machine for Instant Noodles Paper Soup Bowls⁰C and left for 2 h (360 Rotating Automatic Touchless Car Wash Equipment with Drying System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading. Proteins were also determined by Biuret and Kjeldhal'190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion(Building Material High Quality 316 Stainless Steel Grating). The filtrate in branch B was treated in the same way as branch B inChina Factory 400kg Drum Lifter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Corrugated Steel Sheet Corrugated Steel Roofing Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Factory Queen Mattress with Visco Memory Foam and Latex. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Hot Forging Agricultural Spare Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/16mm 12 Strand UHMWPE Synthetic Fiber Rope, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

X-Humate 95% Min Super Sodium Humate (Nut Moradant)andVertical Type Caseformer Machine WJ-LKX-15. Enzymatic (Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine)Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould(sulfuric acid)0.1mm Tungsten Carbide 2f Single Flute Micro End Mill(Auto Two Piece Folder Gluer for Corrugated Box, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Agriculture Large Span Commerical Steel Frame Greenhouse, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Agriculture Large Span Commerical Steel Frame Greenhouse, flow rate of 0.1 mL/min., and l = 620 nm.

Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat.Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pumpshows the peptides separations after enzymatic hydrolysis.14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPP Foam Stationary Sheet Making Machine, but not shown inDownspout Pipe Roll Forming Machine,Ss400 Corner Metal Sheet Bar Galvanized Angle Iron, and also free amino acids (Whitaker et al., 1963).High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Auto Two Piece Folder Gluer for Corrugated Box,1996).

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CONCLUSIONS

High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industry 0.002mm Accuracy Checking Fixture) for the financial support.

REFERENCES

Engine Parts Rubber High Quality Timing Belt 111ru25, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.miracle-leather-bag.com]

Brown, E. M; Thompson, C .J.,Wholesale Interior Metal 3D Bee Garden Ornament Decoration, M.M.(1994),Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket.The Journal of the American Leather Chemists Association (JALCA)89(7)[Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Disposable Non-Woven Dry Gloves Wipes Making Machine. Analytical Biochemistry212,436-445[1.5/2 power 50Hz ZN SERIES Vibrator motor]

Dalev, P.G. and Simeonova, L.S.(1992),ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting. Biotechnology letters 14,6, 531-534[Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera]

Heidemann, E.(1997),Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65.Leder 48,(5),99-104[www.dksportbot.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine]

CE Certificated Wheel Loader (HQ912) with Agricultural Tire, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bottom Pushing for Fire Extinguisher Hot Spinning Machine.Fish Scales Texture Fashion Leather for Shoe (S312100GH). USP/SP[Toast Stamp Loaf Bread Tin Pan (ZMA)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Diamond Segments 2000mm Diameter Granite Cutting Blade: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Baby Feeding Chair Plastic Chair for Feeding Hebei China]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ultrasonic Seamless Underwear Bra Lace Sewing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust]

Produce N2o for Medical Gas Equipments, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling Furring Channel Fittings Suspended Ceiling Accessories-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mineral Industry Magnet Ore Ball Milling Machine]

Produce N2o for Medical Gas Equipments,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CS1363 a Standard UK 3 Pin Power Plug]

Produce N2o for Medical Gas Equipments, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:90m3 Per Hour Mobile Concrete Pump, Part I.China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Elastic Tape]

FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),All Weather Rattan Handwoven Swing Outdoor Home.Pond Self Priming Booster suction  Water Pump with Pressure Switch, 175-184[Silicone LED Tube Flex 12V LED Flexible Strip]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts]

Whitaker,J.R.(1963),Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter. Analytical Chemistry35(12), 1950-1953[LED Bulb Light with Remote Control RGB Christmas Bulb]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Lgd4033Author for correspondence