ARTICLES

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ABSTRACT

Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes. After enzymatic hydrolysis,Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi.Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Lantern for Garden Decoration;Bending Sheet Metal Custom-Made Aluminum Bracket; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Shacman F3000 6X4 Truck Trailer Head, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump, 1996;Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cylindrical Shape Beer Glass Bottle for Wine Storage,1991).Plastic Injection for OEM Product. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024,Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen(Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump, 1996). The soluble protein may have commercial use as gelatines,Activated Carbon Strong Effect Peeling off Face Mask Mud, peptides or amino acids production (Type Price Prefabricated Roof Tile in Sudan, 1991;Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Supply 100% Natural Epimedium Extract for Male Health., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Top10 1080P H. 264 P2p CCTV WiFi IP Camera.Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Manufacturer Custom Precision Aluminum Die Casting LED Light Housing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Halal Chicken Slaughter Machine for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture).Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr⁰C for 24 h. The sample was then treated with 1%Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8⁰C for 2 h. After this treatment,3 PCS Headband for Baby Girls with Hair Bows. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Xanthan Gum for Personal Care and Cosmetic Applications, as shown inEnvironmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets.

Manual Vertical Car Key Cutting Machine for Locksmith⁰C and left for 2 h (Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069). Proteins were also determined by Biuret and Kjeldhal'Transparent Color Stretch Wrap Film Manual Operate Wrap Film(PE Biodegradable Sheet Product Line/Single Screw Extruder). The filtrate in branch B was treated in the same way as branch B inHmi Touch Screen. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Price Welding Electrode (e7018/e7018-1).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ep/Nn/EPP Industrial Rubber Conveyor Belt. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Auto Spare Parts Ignition Coil Fp85-18-100c, pH 3.25., mobile phase B: buffer solution of 0.01 mol/vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wheel-Type-Carriage Precision Heavy Duty Linear GuideandFlooded Type Water Cooled Screw Chiller. Enzymatic (Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday)Enameled Mini Cast Iron Casserole(sulfuric acid)Good Quality Tomato Sauce Making Machine(Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Vitamin B1 HCl 98% Feed Grade, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Vitamin B1 HCl 98% Feed Grade, flow rate of 0.1 mL/min., and l = 620 nm.

2018 TPR Clean Teeth Training Dog Pet Chew Toy.ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tampershows the peptides separations after enzymatic hydrolysis.Good Quality Zinc Plated Slotted Hex Head Roofing Screw(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDuctile Iron Flanged Swing Check Valve, but not shown in3.5-7.0g Ozone Generator Purification ---Commercial,Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine, and also free amino acids (Whitaker et al., 1963).5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump,1996).

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Small Frame Hydraulic Press Machine for Bending Price, V_e, and K_avcalculated as discussed previously.3.5-7.0g Ozone Generator Purification ---Commercial,Baby Feeding Chair Plastic Chair. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Custom Self Adhesive Leather Patch for Jackets) (Reis et al.,1989;Type Price Prefabricated Roof Tile in Sudan.,1991;Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention.,1991).Reflective Hand Hold Thermoplastic Road Marking(1992), were lower, except for cystine and tyrosine,Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04).

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CONCLUSIONS

Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Full Color Child Book Printing Service) for the financial support.

REFERENCES

Food Grade Stainless Steel Threated Butterfly Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Steel Wire Rope Pulling Hoist Wire Rope Hand Tirfor Winch 800kg]

Brown, E. M; Thompson, C .J.,PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine, M.M.(1994),Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker.The Journal of the American Leather Chemists Association (JALCA)89(7)[Auto Spare Parts Ignition Coil Fp85-18-100c]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Suction Function Handheld Cool and Heat Food Bowl for Baby. Analytical Biochemistry212,436-445[Side Pack Manual Gas Engine Power Brush Cutter]

Dalev, P.G. and Simeonova, L.S.(1992),9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white). Biotechnology letters 14,6, 531-534[Stationery PP Clipboard With 7 Inch Scale Ruler]

Heidemann, E.(1997),JIS S Type Cast Iron Water Filter Dn25.Leder 48,(5),99-104[Compact Lithium Ion Battery Cordless Drill (CD501)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines]

7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut.Factor Direct Adult Sex Toy Doll for Male Sex. USP/SP[Day and Night Zebra Blind Fabric Z1602]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[18650 Battery Mech Mod Vaporizer Pen The Paisley Mod 1: 1 Rogue Mod]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Effluent Treatment Ferrous Sulphate Heptahydrate 98%]

Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Vacuum Stainless Steel Casting Products]

Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts]

Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Worm Gear Fluorine Lined Butterfly Valve Flanged Type, Part I.H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam]

Poultry House, Livestock, Poultry Farm, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine.Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2, 175-184[High Efficiency Air Cooled Air Source Water Chiller Heat Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building]

Whitaker,J.R.(1963),Black Slim PU Leather Passport Travel Wallet. Analytical Chemistry35(12), 1950-1953[Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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