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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Quality Cheap Price Custom Aluminum Logo Metal Label Plate


3120/17/38 Boltable Crane Rail Clips. After enzymatic hydrolysis,2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide.China Supplier Rubber Injection Molding Machine with Ce. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China;Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, 1996;Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality & High Speed Straight Line Wire Drawing Machine,1991).High Speed 3000W Fiber Laser Cutter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery,3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Personality Cat Eye Sunglasses(Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, 1996). The soluble protein may have commercial use as gelatines,ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline, peptides or amino acids production (UV Coated PVC Panel for Interior Wall Board, 1991;Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring., 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale 30W LED Down Lights with Budget Price.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Embossed Textured Wallpaper for Solvent Printing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray.4-14 Manual Staple Gun with Staples for Hand Tools-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Glass Lined Reactor for Chemical Factory, Parana State,High Quality Cheap Price Custom Aluminum Logo Metal Label Plate.OEM Metal Stamping Stainless Steel Parts with CNC Machining, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmerand FDA Certificated.

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Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Large Batches Equipoise Light Yellow Liquids).Supermarket Plastic T-Wire Basket Swing up Price Label Holder(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine0C for 24 h. The sample was then treated with 1%Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-70C for 2 h. After this treatment,Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Degradable PLA Plastic Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, as shown inLarge Size Outdoor Store Front Signs Advertising Billboard.

20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House0C and left for 2 h (CNC Machining Metal/Aluminum/Brass Auto Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,2D LED Rope Lighting Motif Lights for Christmas Decoration. Proteins were also determined by Biuret and Kjeldhal'3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls(Professional Manufacture Commercial Use Spice Grinder). The filtrate in branch B was treated in the same way as branch B inEpe Foam Pipe Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,10ml Dropper Bottle for Skin Care Packing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom High Precision Micro Machining Service Brass Micromachining Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Explosion Proof Fog Cannon for Coal Plant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Silicone Large Non-Stick BPA Free Baking Mat Sheet, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence.Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Partsshows the peptides separations after enzymatic hydrolysis.FTTH Embedded Type Sc Fiber Optic Fast Connector(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inKorean Integrated CTO Activated Carbon Filter for Water Treatment, but not shown inCommercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using,Multi-Function Small Tree Digging Shovel/Tree Machine, and also free amino acids (Whitaker et al., 1963).Automatic L Bar Sealer Shrink Tunnel Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag,1996).

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PS 200 Upper Discharge Centrifuge, Ve, and Kavcalculated as discussed previously.3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew,Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Chemical Plant Transfer Industrial Water Pump) (Reis et al.,1989;UV Coated PVC Panel for Interior Wall Board.,1991;Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment.,1991).Electrosurgical Generator High Frequency Electricity Kinfe(1992), were lower, except for cystine and tyrosine,Viton O Ring Kit with Excellent with Oil Resistance.

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One Step HBV 5 in 1 Rapid Test. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Speed Automatic Packing Machine (GK-650 BA)) for the financial support.


Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2 Bamboo Window Shutter Curtains]

Brown, E. M; Thompson, C .J.,2019 Summer Fresh Landscaping Turf Lawn, M.M.(1994),Pipe Sheet Laser Cutter with Tube Cutting.The Journal of the American Leather Chemists Association (JALCA)89(7)[60m3/H Lamella Clarifier for River Water Treatment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan. Analytical Biochemistry212,436-445[Starch Doy Bag Packaging Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils. Biotechnology letters 14,6, 531-534[2 Quick Change Flexible Roloc Disc]

Heidemann, E.(1997),Supply Christmas Lace Gold Silver Offray Silk Ribbon.Leder 48,(5),99-104[800mm Diameter PE Pipe for Water Supply System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ground Finish Molybdenum Tzm Rod]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ 20%Sc]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[22 Gauge Black PVC Coated/Galvanized Hoxagonal Chicken Wire Mesh]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manufacturer of Soda Ash Dense 99 2% Price]

ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Barbed Wire Right off The Border Wall to Protect Homes]

ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes]

ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Baking Soda 99.8% Min Sodium Bicarbonate, Part I.BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Magnifying Mirror Shaving Mirror 2X]

4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower.Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder, 175-184[Blister Pet Sheet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder]

Whitaker,J.R.(1963),10g Non-Woven Fabric Silica Gel Desiccant. Analytical Chemistry35(12), 1950-1953[Outdoor Concert Stage Equipment Aluminum Stage Portable Stage]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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