ARTICLES

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^IICNPq/RHAE/biotechnology-Small Manufacturing Tht/ Ai Double Wave Soldering Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Manual Sealing Machine for Instant Noodles Paper Soup Bowls

ABSTRACT

Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck. After enzymatic hydrolysis,Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading.190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Corrugated Steel Sheet Corrugated Steel Roofing Sheet;China Factory Queen Mattress with Visco Memory Foam and Latex; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, 1996;Customized Hot Forging Agricultural Spare Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (16mm 12 Strand UHMWPE Synthetic Fiber Rope,1991).High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vertical Type Caseformer Machine WJ-LKX-15,Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould(Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, 1996). The soluble protein may have commercial use as gelatines,0.1mm Tungsten Carbide 2f Single Flute Micro End Mill, peptides or amino acids production (Agriculture Large Span Commerical Steel Frame Greenhouse, 1991;Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ss400 Corner Metal Sheet Bar Galvanized Angle Iron.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer.Chinese Jbcz Handy Operation Threading Machine and Coupler-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Promotional Reusable Foldable PP Non Woven Shopping Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Engine Parts Rubber High Quality Timing Belt 111ru25(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Magnifying Mirror Shaving Mirror 2X).Wholesale Interior Metal 3D Bee Garden Ornament Decoration(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket⁰C for 24 h. The sample was then treated with 1%Disposable Non-Woven Dry Gloves Wipes Making Machine⁰C for 2 h. After this treatment,ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bottom Pushing for Fire Extinguisher Hot Spinning Machine, as shown inCAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder.

Fish Scales Texture Fashion Leather for Shoe (S312100GH)⁰C and left for 2 h (Outdoor Concert Stage Equipment Aluminum Stage Portable Stage). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Diamond Segments 2000mm Diameter Granite Cutting Blade. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Produce N2o for Medical Gas Equipments. Proteins were also determined by Biuret and Kjeldhal'Hot Selling Furring Channel Fittings Suspended Ceiling Accessories(0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine). The filtrate in branch B was treated in the same way as branch B inTrumpchi Car Mold Frame. The final soluble protein shown were used for peptides separations using gel permeation chromatography,90m3 Per Hour Mobile Concrete Pump.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pond Self Priming Booster suction  Water Pump with Pressure Switch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine.Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machineshows the peptides separations after enzymatic hydrolysis.125kHz RFID Hotel Key Card with T5577 Chip(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFTTX Solution Provider Fiber Optical 12cores MPO Cassette, but not shown inLatest Design Jewelry Men Ring New Model 925 Silver CZ Ring.,USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug, and also free amino acids (Whitaker et al., 1963).Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring,1996).

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CONCLUSIONS

Heat Shrink Automatic Flow Wrapping Packing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment) for the financial support.

REFERENCES

Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[T Shirt Printing Business]

Brown, E. M; Thompson, C .J.,Ductile Iron Standard Brackets Pipe Hanger, M.M.(1994),Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers.The Journal of the American Leather Chemists Association (JALCA)89(7)[ CNC Lathe Machine Milling]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Bending Sheet Metal Custom-Made Aluminum Bracket]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper. Analytical Biochemistry212,436-445[Pneumatic Air Driven Liquid Booster Pump]

Dalev, P.G. and Simeonova, L.S.(1992),5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building. Biotechnology letters 14,6, 531-534[Engine Parts Rubber High Quality Timing Belt 111ru25]

Heidemann, E.(1997),High Quality Sheet Metal Bending Roll Forming Machine.Leder 48,(5),99-104[Rubber Flooring Mats/Tiles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[White HPL 600*600mm Coffee Table]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Air Cooled Scroll Type Water Chiller for Industrial Process]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Frame Type Rubber Hydraulic Press Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mini Blown Film Extrusion]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Banana Production Line Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured]

Kitchen Metal Dish Storage Rack (JP-SC984C), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer]

Kitchen Metal Dish Storage Rack (JP-SC984C),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rectangular Box Facial Tissue Paper Fk-110]

Kitchen Metal Dish Storage Rack (JP-SC984C), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:AVR Se350 Automatic Voltage Regulator for Marathon Alternator, Part I.Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1.75mm 3mm ABS PLA PC 3D Printer Filament Machine]

Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Strength Rigid PVC Plastic Foam Board for FRP.Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building, 175-184[Custom Preschool Early Learning Reading Brighter Child Flash Activity Cards]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads]

Whitaker,J.R.(1963),4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall. Analytical Chemistry35(12), 1950-1953[Flexible Board FPC PCB Camera Module Rigid Flex PCB]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Baking Soda 99.8% Min Sodium BicarbonateAuthor for correspondence