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ABSTRACT

Glass Door Passenger Sightseeing Elevator for Sale. After enzymatic hydrolysis,Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys.12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple;Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fcy30 Transport Mini Tipping Dumper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, 1996;Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Premium Grade Rubber Cloth Duct Tape,1991).Sud1000h Poly Welder Butt Fusion Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Anti-Rust Torsion Spring,Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt(Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, 1996). The soluble protein may have commercial use as gelatines,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, peptides or amino acids production (OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic, 1991;PP Woven Bag, Rice, Beans, Plastic Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Professional Mold Factory Plastic Injection Mould Maker., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Industrial Carbon Steel Wire Hose Hydraulic Pipe.24k Gold Whitening Anti-Spot Mask-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Clay Tiles Making Machine with Latest Technology(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

DIN Standard Gratings/Gavlanized Steel GratingAlcalase2.5 L DX,50mm Magnetic River Water Sewage Flow Meter for Acid, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-PE Biodegradable Sheet Product Line/Single Screw Extruder); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)27L/Min Water Exchanger Heat Pump Mold Temperature Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Felt Belt for Textile Compactor, Parana State,7inch Industrial Tablet Computer Naked Resistance Touch Screen.Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashion Mens Long Zipper Credit Card Purse PU Phone Wallet with StrapandSquare Premium Glass Perfume Bottle 100ml.

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Metal Soft Enamel Souvenir Metal Medal Sport Medallions(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Xjm Series Rubber Extruding Machine).20L Water Tank Lid Mold 3D Printing Samples Available(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale High Quality Metal Car Mount Holder Phone Holder⁰C for 24 h. The sample was then treated with 1%Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch⁰C for 2 h. After this treatment,High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pet Laminate Aluminium Foil/Pet+Alu+PE's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale, as shown inHigh Quality 500m 100lb Super Strong Braided Fishing Line.

New Design Multi-Color Silicone Coin Purse for Lady⁰C and left for 2 h (China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen. Proteins were also determined by Biuret and Kjeldhal'Dark Green Plastic PVC Film for Christmas Tree Leaves(Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier). The filtrate in branch B was treated in the same way as branch B inLCD Display Arm Digital Blood Pressure Monitor for Elderly. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Water Treatment Anthracite(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heat Treated Welding Protection Glassfiber Cloth Ht800⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

OEM Factory Balloon Padding Textile MachineryandHeat Pump Non-Programmable HVAC Room Thermostat. Enzymatic (Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo)Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable(sulfuric acid)Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer(Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, flow rate of 0.1 mL/min., and l = 620 nm.

Injector Pump Calibration Test Bench.High Quality Inflatable PVC Flocking Health Bedshows the peptides separations after enzymatic hydrolysis.ASME/ASTM Butt-Welding Stainless Steel Plate Flange(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWhite 4*8FT 18mm PVC Foam Board for Cabinets Use, but not shown inContinual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil,500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank, and also free amino acids (Whitaker et al., 1963).Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park,1996).

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CNC Machining Checking Auto Car Checking FixtureandMs Gi Pipe Dn15~Dn400 Gi Pipe Listshow the gel chromatographic separation of soluble protein with Sephadex G-Polyester / Polypropylene Woven Geotextile for Road Construction.Granite Stone Jaw Crusher of Mining Machine, and another peak at 45 mL not shown in1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, for peptides lower than 3000 Da.Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor(Condell et al.,1993) .

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Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer, V_e, and K_avcalculated as discussed previously.The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine,Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple) (Reis et al.,1989;OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic.,1991;Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250).,1991).Wholesale All Kinds Cheap Plush Animal Baby Slippers(1992), were lower, except for cystine and tyrosine,Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car.

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CONCLUSIONS

Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PE Biodegradable Sheet Product Line/Single Screw Extruder) for the financial support.

REFERENCES

Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Semi-Hermetic Compressor Unit]

Brown, E. M; Thompson, C .J.,New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress, M.M.(1994),Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Original Stainless Steel Singer Brand Sewing Machine Needles]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Taiding Custom Tank Sheet Metal Fabrication. Analytical Biochemistry212,436-445[19mic Black Cast PE Stretch Film for Pallet Wrapping]

Dalev, P.G. and Simeonova, L.S.(1992),Airtight Candy Bin with Scoop. Biotechnology letters 14,6, 531-534[Imee Print Customized Printing Agenda Printing Nb21]

Heidemann, E.(1997),Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar.Leder 48,(5),99-104[Custom Stampings Stainless Steel Sheet Metal Fabrication]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving]

Custom Grey Full-Zip Cotton Mens Hoodies, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell.3 Layer Round Tea Tin with Air Tight Lid. USP/SP[Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China PA System Power Amplifier with Enough Rated Power: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[200kw Medium Frequency Induction Furnace for Melting Scrap Iron Steel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making]

Export Grade Citric Acid Monohydrate as Detergent, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Wire Diameter 18AWG]

Export Grade Citric Acid Monohydrate as Detergent,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3 Layer Round Tea Tin with Air Tight Lid]

Export Grade Citric Acid Monohydrate as Detergent, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Familu Use Mini Small Pig Feed Pellet Mill, Part I.3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Selling Furring Channel Fittings Suspended Ceiling Accessories]

Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed.Hairdressing Equipment Takara Belmont Barber Chair High Quality, 175-184[Diesel Injector Filter/Knitted Wiremesh/Mister Screen]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes]

Whitaker,J.R.(1963),Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000). Analytical Chemistry35(12), 1950-1953[1.3KG Fire breaker for kitchen]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

15ml 18ml Glass Fish Model Perfume Bottle with Plastic SprayerAuthor for correspondence