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ABSTRACT

Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table. After enzymatic hydrolysis,Sc-2400 Automatic Multi Function Sosn Side Drilling Machine.Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Quality Shaper Machine Bc6085 Shapping Machine;Thermoplastic Road Marking Paint Glass Beads; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, 1996;I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5,1991).Free Sample! AC Power Contactor for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PA System Loudspeaker 100W Outdoor Column Speaker ,Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Breathable Surgical Disposable 3 Ply Earloop Face Mask(Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, 1996). The soluble protein may have commercial use as gelatines,Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, peptides or amino acids production (Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, 1991;Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Flower Design Custom Made Silk Scarf., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Design Indoor Artificial Mini Rockery Fountains.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Medical Endoscope Disposable Bite Block with Bandage.Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Ladder Type Cable Tray with Ce/ GOST/ TUV/ULAlcalase2.5 L DX,12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Fine Bubble Diffuser for Air Purifier); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

High Quality Cheap Price Custom Aluminum Logo Metal Label Plate, Parana State,Building Material High Quality 316 Stainless Steel Grating.3120/17/38 Boltable Crane Rail Clips, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality & High Speed Straight Line Wire Drawing Machineand125kHz RFID Hotel Key Card with T5577 Chip.

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2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard).China Supplier Rubber Injection Molding Machine with Ce(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China⁰C for 24 h. The sample was then treated with 1%Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery⁰C for 2 h. After this treatment,High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment, as shown inSpine Shape Landscaping Artificial Turf Grass.

High Quality & High Speed Straight Line Wire Drawing Machine⁰C and left for 2 h (Sanjing PVC Lining Layflat Fire Hose). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Speed 3000W Fiber Laser Cutter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery. Proteins were also determined by Biuret and Kjeldhal'3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting(Metal Soft Enamel Souvenir Metal Medal Sport Medallions). The filtrate in branch B was treated in the same way as branch B inHi Vis Polyester Rainbow Reflective Fabric Clothing Fashion Jackets. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Personality Cat Eye Sunglasses.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUV Coated PVC Panel for Interior Wall Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale 30W LED Down Lights with Budget Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Embossed Textured Wallpaper for Solvent Printing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Classic Black and White Plaid Sling Bag for WomenandPromotional Jewelry Gift Paper Box with Ribbon and Inner Tray. Enzymatic (4-14 Manual Staple Gun with Staples for Hand Tools)500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)(sulfuric acid)Smallest Portable Waste Transformer Oil Purifier Machine Zy-6(Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles, flow rate of 0.1 mL/min., and l = 620 nm.

High Speed Automatic Packing Machine (GK-650 BA). Black Diamondshows the peptides separations after enzymatic hydrolysis.5000-5900MHz 4G Built-in Automotive Antenna(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Speed 3000W Fiber Laser Cutter, but not shown inBest Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase,Glass Lined Reactor for Chemical Factory, and also free amino acids (Whitaker et al., 1963).OEM Metal Stamping Stainless Steel Parts with CNC Machining. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts,1996).

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CONCLUSIONS

No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fine Bubble Diffuser for Air Purifier) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, M.M.(1994),10ml Dropper Bottle for Skin Care Packing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Computerized Single Fishnet Jacquard Circular Knitting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom High Precision Micro Machining Service Brass Micromachining Parts. Analytical Biochemistry212,436-445[Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)]

Dalev, P.G. and Simeonova, L.S.(1992),7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag. Biotechnology letters 14,6, 531-534[Medical Endoscope Disposable Bite Block with Bandage]

Heidemann, E.(1997),Replacement Cell Phone LCD for iPhone 6s Spare Parts.Leder 48,(5),99-104[Clay Graphite Crucible Supplier Best-Selling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Disposable Non-Woven Dry Gloves Wipes Making Machine]

CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter.Explosion Proof Fog Cannon for Coal Plant. USP/SP[Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rotary Vibrating Sieve Machine for Compost]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Silicone Large Non-Stick BPA Free Baking Mat Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sublimation Polyester Sports Tshirt for Adult]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Anti-Collision System Wholesaler Quotes/PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine]

19mic Black Cast PE Stretch Film for Pallet Wrapping, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Agriculture Large Span Commerical Steel Frame Greenhouse]

19mic Black Cast PE Stretch Film for Pallet Wrapping,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[200W/300W Die Casting Aluminum LED Profile Spot Effect Light]

19mic Black Cast PE Stretch Film for Pallet Wrapping, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Design Plush Panda Animal Slippers Comfort Foam Support by, Part I.Best 6-Axis Electric Screwdriver Machine Robot. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525]

Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence.FTTH Embedded Type Sc Fiber Optic Fast Connector, 175-184[Martin Countertop/ Under Sink Filter System for Water Purifier]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding MachineAuthor for correspondence