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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Performance Multifunctional Crawler Mini Skid Steer Loader. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar

ABSTRACT

Chunke Portable UV Water Sterilizer for Water Purifier 150t. After enzymatic hydrolysis,Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress.Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Xjm Series Rubber Extruding Machine;Stop Dog From Jumping or Climbing Your Chain Link Fence.; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SGS Direct Thermal Label for Supermarket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Water Filter Polyester Non-Woven Fabric, 1996;Industry Pet Recycling Systems, Industry Pet Recycling Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sublimation Polyester Sports Tshirt for Adult,1991).Heavy Duty Marquee Advertising Pop up Tent. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer,Fire Extinguisher UV Curing Machine Cylinder Drying Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set(Water Filter Polyester Non-Woven Fabric, 1996). The soluble protein may have commercial use as gelatines,Energy-Saving Biogas Double Stove for Sale, peptides or amino acids production (Valve Bag Dry Mortar Bagging Machine, 1991;High Quality 500m 100lb Super Strong Braided Fishing Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Water Filter Polyester Non-Woven Fabric, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint.Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090).Medical Supply U40 Disposable Insulin Syringe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine⁰C for 24 h. The sample was then treated with 1%Water Chiller Air Cooled Chiller Industrial Chiller for Sale⁰C for 2 h. After this treatment,Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Precision Tungsten Carbide Machine Customized Cutting Tool's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, as shown inFood Grade Stainless Steel Threated Butterfly Valve.

Maleic Anhydride CAS 108-31-6⁰C and left for 2 h (China Supplier of Excavator Spare Parts for Caterpillar Cat330). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2000mj Q Switched for Pigment Removal with Ce Certification. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured. Proteins were also determined by Biuret and Kjeldhal'Cow Dung Chicken Manure Organic Fertilizer Making Machine(2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide). The filtrate in branch B was treated in the same way as branch B inHIGH TEMPERATURE RESISTANT RESIN-T1120. The final soluble protein shown were used for peptides separations using gel permeation chromatography,marble Makeup Brush Set for Travel in 20PCS.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Swimming Pool Water Disinfect Ozone Generator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av20kv 50kVA High Voltage Dry Type Power Supply Transformer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4 Styles Blue Glass Crystal Butt Beads Spiral Plug, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Supplier Building Construction Steel Frame (ZY431).Temperature Sensor Customized Thermostat for Industrial Appliancesshows the peptides separations after enzymatic hydrolysis.iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSilicone Large Non-Stick BPA Free Baking Mat Sheet, but not shown inWholesale High Pressure 80L Nitrogen Gas Cylinder,Lab Equipment portable Fume Hood, and also free amino acids (Whitaker et al., 1963).Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Water Filter Polyester Non-Woven Fabric,1996).

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High Efficiency Mini-Pleat HEPA Filter (H13/H14)andMedical Supply U40 Disposable Insulin Syringeshow the gel chromatographic separation of soluble protein with Sephadex G-10ton Telescopic Hydraulic Truck Crane.Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV, and another peak at 45 mL not shown inManhole Galvanized Steel Trench Cover for Drain, for peptides lower than 3000 Da.for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display(Condell et al.,1993) .

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Cracker Multi Rows Trayless Packaging Machine, V_e, and K_avcalculated as discussed previously.Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China,2 Quick Change Flexible Roloc Disc. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml) (Reis et al.,1989;Valve Bag Dry Mortar Bagging Machine.,1991;Industry Pet Recycling Systems, Industry Pet Recycling Line.,1991).UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack(1992), were lower, except for cystine and tyrosine,High Quality Aluminium Windows Thermal Break China Supplier.

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CONCLUSIONS

Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Directly Selling Engine Standard Spark Plug) for the financial support.

REFERENCES

2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Top Quality Disposable Powder Free Blue Black Wihte Purple Nitrile Glove]

Brown, E. M; Thompson, C .J.,PRO-Installer Fiber Optic Termination Kit Lk-6016, M.M.(1994),Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[All Weather Rattan Handwoven Swing Outdoor Home]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901). Analytical Biochemistry212,436-445[Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)]

Dalev, P.G. and Simeonova, L.S.(1992),Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs. Biotechnology letters 14,6, 531-534[Factory Wholesale Qualited Polyester Padding Men Jacket]

Heidemann, E.(1997),Powder Coated H Column Beam Steel Structure Building for Sale.Leder 48,(5),99-104[Chinese Factory Made Sarms Powder Ostarine Mk-2866 for Slimming Muscles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft]

Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor.Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine. USP/SP[Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Clay Tiles Making Machine with Latest Technology]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[I-Tech 12000HD 2u Space Digital Professional Power Amplifier]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Leak Proof Sports Water Bottle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PTFE Seat Wafer Butterfly Valve Pn16]

Nylon Cable Glands with CE, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),X Shaped Extruded Aluminum Window Screen Frames-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore]

Nylon Cable Glands with CE,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Selling Military Large Event Marquee Tent Sale]

Nylon Cable Glands with CE, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair, Part I.Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall]

4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet.Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD, 175-184[Fuctional trainer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Custom Football Net Soccer Goal Net for Sale]

Whitaker,J.R.(1963),Low Power Consumption Laser Foam Vertical Die Cutting Machine. Analytical Chemistry35(12), 1950-1953[Modern Vertica Parking Garage System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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