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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,OEM Logo Soft Silicone Reusable BPA Free Drinking Straws

ABSTRACT

Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin. After enzymatic hydrolysis,4 Drawer Steel Office Vertical Filing Cabinet.12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Parts for Crane Outrigger Hydraulic Oil Cylinder;Boned Polyester Woven Faux Suede Sofa Fabric; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (20 Bottles Electric Wine Display Cooler, 1996;High-Efficient Htk Walnut Shell Filter for Water Purification., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576),1991).Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Popular European Style White Marble Coffee Tables for Dinnig Room,High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System(20 Bottles Electric Wine Display Cooler, 1996). The soluble protein may have commercial use as gelatines,Kwikstage Scaffolding System-Diagonal Brace, peptides or amino acids production (360 Rotating Automatic Touchless Car Wash Equipment with Drying System, 1991;48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sell Rubber / EVA / PE/PVC Foam Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (20 Bottles Electric Wine Display Cooler, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sodium Gluconate 98% Min Industry Grade for Chemical Admixture.Brake Disc-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

25kg 50kg Plastic PP Woven Feed Bags, Parana State,OEM Logo Soft Silicone Reusable BPA Free Drinking Straws.360 Degree Rotation Medical Seat Cushion, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (20 Bottles Electric Wine Display Cooler, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theKorean Integrated CTO Activated Carbon Filter for Water TreatmentandCommercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using.

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Custom LCD Screen Video Box for Package(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price).Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning⁰C for 24 h. The sample was then treated with 1%2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine⁰C for 2 h. After this treatment,Alu 2319 Slimline 12mm Wide Recessed Profile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Size Christmas Gift Personalized Watch Boxes for Men, as shown inPromotional Customized PP Hand Fan.

12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope⁰C and left for 2 h (Auto Plastic Part Hardware Plastic Injection Mold). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality. Proteins were also determined by Biuret and Kjeldhal'New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115(Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine). The filtrate in branch B was treated in the same way as branch B inChinese Scooter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Cross Recesed Flat Head Self Tapping Screw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOur Perforated Aluminum & Solid Panel for New Office Wall Cladding(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Kids Trucker Hat, Kids Mesh Cap⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wd190V Handheld Full Body Scanner Hand Held Metal Detector, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Chemical Plant Transfer Industrial Water PumpandHydraulic Orbital Valve Power Steering. Enzymatic (W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine)AISI 410 Stainless Steel Galvanized Wire for Making Scourer(sulfuric acid)CS1363 a Standard UK 3 Pin Power Plug(20 Bottles Electric Wine Display Cooler, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Food Chopper, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Food Chopper, flow rate of 0.1 mL/min., and l = 620 nm.

Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile.SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospitalshows the peptides separations after enzymatic hydrolysis.Wholesale Prices of 20W 40W 60W Solar Street Lights(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBest Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector, but not shown in60m3/H Lamella Clarifier for River Water Treatment,ISO 3821 3/16′′ Twin Line Welding Hose for USA Market, and also free amino acids (Whitaker et al., 1963).Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (20 Bottles Electric Wine Display Cooler,1996).

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Starch Doy Bag Packaging Machineand2 Quick Change Flexible Roloc Discshow the gel chromatographic separation of soluble protein with Sephadex G-Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond.Factory Stock Spring Steel U Type Clip Nut, and another peak at 45 mL not shown inHolo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, for peptides lower than 3000 Da.ASME VIII Pressure Vessel for Curing Carbon Fiber Parts(Condell et al.,1993) .

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Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, V_e, and K_avcalculated as discussed previously.Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut,Portable Electric Concrete Vibrator with 18000 High Rotation Speed. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ground Finish Molybdenum Tzm Rod) (Reis et al.,1989;360 Rotating Automatic Touchless Car Wash Equipment with Drying System.,1991;High-Efficient Htk Walnut Shell Filter for Water Purification.,1991).22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster(1992), were lower, except for cystine and tyrosine,Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122.

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CONCLUSIONS

Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder) for the financial support.

REFERENCES

Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Medical Hard Clear Transparent Empty Gelatin Capsule Size 00]

Brown, E. M; Thompson, C .J.,UCP Color Army Camouflage Uniform Army Military Uniform Suit, M.M.(1994),0.5 Gallons Cool Mist Ultrasonic Humidifier.The Journal of the American Leather Chemists Association (JALCA)89(7)[Barbed Wire Right off The Border Wall to Protect Homes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates. Analytical Biochemistry212,436-445[Magnifying Mirror Shaving Mirror 2X]

Dalev, P.G. and Simeonova, L.S.(1992),Sanjing PVC Lining Layflat Fire Hose. Biotechnology letters 14,6, 531-534[CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder]

Heidemann, E.(1997),Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar.Leder 48,(5),99-104[3 Ton Electric Chain Hoist Electric Block with Trolley]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor Concert Stage Equipment Aluminum Stage Portable Stage]

Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light.Automatic CNC Glass Cutting Machine Glass Cutting Table. USP/SP[0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.ymspcb.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[FTTX Solution Provider Fiber Optical 12cores MPO Cassette]

Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.]

Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved]

Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pet PP Rope and Broom Wire Drawing Machine, Part I.14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Instax Mini Camera Korea Style Photo Album]

PTFE Seat Wafer Butterfly Valve Pn16, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part.China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, 175-184[Circuit Board PCB Manufacturer for Electronics]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sterile Dsiposable Syringe for Dental 12ml]

Whitaker,J.R.(1963),Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China. Analytical Chemistry35(12), 1950-1953[Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industrial Rubber Nylon Conveyor Belting Automotive PartsAuthor for correspondence