ARTICLES

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ABSTRACT

Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01). After enzymatic hydrolysis,Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt.Plain Makeup Bag Canvas Cosmetic Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry, 1996;Factory Wholesale Qualited Polyester Padding Men Jacket., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam,1991).Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration,Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat(Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry, 1996). The soluble protein may have commercial use as gelatines,River Water Cleaning Sludge Sediment Machine Lamella Clarifier, peptides or amino acids production (Polyester Coated Stainless Steel Ladder Type Metal Ties, 1991;2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Copper Laminated Steel Strip T2/C11000.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Halal Empty Gelatin Capsule for Medicine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm.Gym Fitness Exercise Sporting Body Building Bicep Press-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2*2 4G Lte MIMO Antenna Rrouter/CPE).Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver⁰C for 24 h. The sample was then treated with 1%Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank⁰C for 2 h. After this treatment,Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plastic Food Box Container Covers by Injection Mould Mold Tool's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment, as shown inSinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale.

Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans⁰C and left for 2 h (Chunke Portable UV Water Sterilizer for Water Purifier 150t). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Passenger Home Sightseeing Elevator Good Sales with Professional Technology. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Vacuum Stainless Steel Casting Products. Proteins were also determined by Biuret and Kjeldhal'Chicken Farming Materials Poultry Battery Cages Layer Battery Cage(Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry). The filtrate in branch B was treated in the same way as branch B inTable Barcode Scanner Module Supplier & Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%I-Tech 12000HD 2u Space Digital Professional Power Amplifier. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Polyurethane Gasket Foam Seal Dispensing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Imee Print Customized Printing Agenda Printing Nb21, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cute Clear Fanny Pack Stadium Security PVC Waist BagandHigh Performance Multifunctional Crawler Mini Skid Steer Loader. Enzymatic (Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar)Chunke Portable UV Water Sterilizer for Water Purifier 150t(sulfuric acid)Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress(Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

High Quality 500m 100lb Super Strong Braided Fishing Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile) for the financial support.

REFERENCES

20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Low Price Cofdm Mini Covert Mobile Video Transmitter]

Brown, E. M; Thompson, C .J.,37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light, M.M.(1994),Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags.The Journal of the American Leather Chemists Association (JALCA)89(7)[Humic Acid Bio-Fertilizer Granule Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint. Analytical Biochemistry212,436-445[China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)]

Dalev, P.G. and Simeonova, L.S.(1992),Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine. Biotechnology letters 14,6, 531-534[Nylon Balance Bike with Water Transfer Printing Malaysia]

Heidemann, E.(1997),Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending.Leder 48,(5),99-104[hbzhongrunfelt]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV]

Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch.Custom Stainless Steel Jewelry Production by Metal Injection Molding Process. USP/SP[Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Directly Selling Engine Standard Spark Plug: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Шаровая Мельница]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[FSTN Type Monochrome Graphic Display Cog LCD with Backlight]

Custom Logo Silicone Wristband, Rubber Wrist Bands, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom All Kinds of Acrylic Shoe Display Racks-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame]

Custom Logo Silicone Wristband, Rubber Wrist Bands,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag]

Custom Logo Silicone Wristband, Rubber Wrist Bands, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide, Part I.Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK]

Whitaker,J.R.(1963),Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair. Analytical Chemistry35(12), 1950-1953[DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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