ARTICLES

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ABSTRACT

Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera. After enzymatic hydrolysis,Hot Sale Power Press Perforated Metal Punch Machine.100%Polyester Satin Fabric for Blouse. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High-End Compression Rash Guards, Long Sleeve Compression Shirt, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo, 1996;Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters,1991).9~12 Inch Mechanical Backing Plate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,DIN929 Low-Cost Supply Hexagon Weld Nut,Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Efficiency Solar Cell Photovoltaic 300W Hot Sell(High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo, 1996). The soluble protein may have commercial use as gelatines,60m3/H Lamella Clarifier for River Water Treatment, peptides or amino acids production (SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019), 1991;Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Soldering Machine LED Module PCB Hot Air Soldering Station.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W.High Safety Double Wire Security Metal Welded Wire Mesh Fence-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pneumatic Air Driven Liquid Booster Pump(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hemp Seed Oil Body Lotion Moisturizes The Skin(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2).SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal⁰C for 24 h. The sample was then treated with 1%UTP CAT6 48 Ports Vertical Termination Patch Panel⁰C for 2 h. After this treatment,Prefab Expandable Luxury Living Container House Prefabricated House. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, as shown in29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Small Steel Silo for Poultry Feed. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGood Price Nasal Paste Packaging Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Low-E Insulating Glass Curtain Wall.Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toysshows the peptides separations after enzymatic hydrolysis.Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCE Certificated Wheel Loader (HQ912) with Agricultural Tire, but not shown inHigh Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns,Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D), and also free amino acids (Whitaker et al., 1963).Fashion Design Underwear for Men Cotton Mens Boxer Briefs. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo,1996).

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CONCLUSIONS

Auto Car Accessories Seat for Child. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK) for the financial support.

REFERENCES

Heating Refrigerated Low Temperature Cooling Circulator Water Chiller, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Horizontal Full Automatic Stick Sugars Packaging Machine Price]

Brown, E. M; Thompson, C .J.,Zipper Pouch Pistachio Nuts Premade Packing Machine, M.M.(1994),Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear.The Journal of the American Leather Chemists Association (JALCA)89(7)[0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rebonded Foam Machina]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer. Analytical Biochemistry212,436-445[Vitamin B1 HCl 98% Feed Grade]

Dalev, P.G. and Simeonova, L.S.(1992),Colombia Outdoor P5 Pantallas LED Display. Biotechnology letters 14,6, 531-534[Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)]

Heidemann, E.(1997),2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch.Leder 48,(5),99-104[Factory Make Fashion Antique Stainless Steel Women Watch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Atf-805 Auto Transmission System Flushing Machine with CE/SGS]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Chicken Nipple Drinker System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Salt Spray Corrosion Testing Machine/Salt Spray Test Chamber]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminum Low Profile Adjustable Bracket]

Industrial Hydraulic Ice Grape Press Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Common Rail Diesel Injector Nozzle Dlla153p977-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump]

Industrial Hydraulic Ice Grape Press Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster]

Industrial Hydraulic Ice Grape Press Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Betaine 98% Betaina Feed Grade, Part I.Glass Salt & Pepper Mill/Grinder Glass Spice Jar. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber]

Outsole Trimming Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel.Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01), 175-184[A4 Uv Printer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pen Type pH Meter pH-031&0.00-14.00pH Tester]

Whitaker,J.R.(1963),CE Certificated Wheel Loader (HQ912) with Agricultural Tire. Analytical Chemistry35(12), 1950-1953[Stamping Metal Parts Manufacturer Sheet Parts Fabrication]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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