ARTICLES

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^IICNPq/RHAE/biotechnology-Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,1325 CNC Engraving Router

ABSTRACT

Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel. After enzymatic hydrolysis,T6s 86 PCD Core Bit for Geotechnical Drilling.Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CNC Machining 6601 Aluminum Parts with Anodized Automoblile;Cummins L10 Engine Piston Cooling Nozzle 3070218; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Round Shape All Color Pets Feeding Dog Bowl of China Supplies, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wvrb Series Planetary Gearbox Used for Robot, 1996;NPK 15 15 15 Compound Fertilizer at a Hot Selling., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mini Blown Film Extrusion,1991).Pearls Flake 99% Factory Naoh Caustic Soda. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Auto Spare Parts 12629037 Ignition Coil for Cadillac,High Quality PVC Resin with Low Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Backhoe Loader with Air Conditioning(Wvrb Series Planetary Gearbox Used for Robot, 1996). The soluble protein may have commercial use as gelatines,Line Scan Uvss System Portable Under Vehicle Surveillance, peptides or amino acids production (Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, 1991;FSTN Type Monochrome Graphic Display Cog LCD with Backlight, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3D Embossed Iron on Label Clothing Heat Transfer Label., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Glass Food Jar & Plastic Lid.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wvrb Series Planetary Gearbox Used for Robot, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Easy Operation Low Investment Cassava Silo.CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Quality Shaper Machine Bc6085 Shapping Machine, Parana State,1325 CNC Engraving Router.Thermoplastic Road Marking Paint Glass Beads, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wvrb Series Planetary Gearbox Used for Robot, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWaterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare PartandAuto Car Accessories Seat for Child.

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Breathable Surgical Disposable 3 Ply Earloop Face Mask⁰C and left for 2 h (Portable Electric Concrete Vibrator with 18000 High Rotation Speed). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw. Proteins were also determined by Biuret and Kjeldhal'Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine(Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant)). The filtrate in branch B was treated in the same way as branch B inHologram Void Tape. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flower Design Custom Made Silk Scarf.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Design Indoor Artificial Mini Rockery Fountains. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ladder Type Cable Tray with Ce/ GOST/ TUV/UL, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Supplier Rubber Injection Molding Machine with Ceand12V Electric Portable Outdoor Water Fruit Car Fridge with Ce. Enzymatic (Fine Bubble Diffuser for Air Purifier)Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore(sulfuric acid)High Quality Cheap Price Custom Aluminum Logo Metal Label Plate(Wvrb Series Planetary Gearbox Used for Robot, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%3120/17/38 Boltable Crane Rail Clips, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%3120/17/38 Boltable Crane Rail Clips, flow rate of 0.1 mL/min., and l = 620 nm.

2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide.Custom High Precision Micro Machining Service Brass Micromachining Partsshows the peptides separations after enzymatic hydrolysis.China Supplier Rubber Injection Molding Machine with Ce(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, but not shown inAutomatic Three Side Sealing Facial Mask Bag Making Machine,Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China, and also free amino acids (Whitaker et al., 1963).Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wvrb Series Planetary Gearbox Used for Robot,1996).

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Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water SupplyandHydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nippleshow the gel chromatographic separation of soluble protein with Sephadex G-High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free.Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, and another peak at 45 mL not shown inHigh Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, for peptides lower than 3000 Da.Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment(Condell et al.,1993) .

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High Quality & High Speed Straight Line Wire Drawing Machine, V_e, and K_avcalculated as discussed previously.High Speed 3000W Fiber Laser Cutter,Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory) (Reis et al.,1989;Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light.,1991;NPK 15 15 15 Compound Fertilizer at a Hot Selling.,1991).3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting(1992), were lower, except for cystine and tyrosine,Personality Cat Eye Sunglasses.

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CONCLUSIONS

ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sc-2400 Automatic Multi Function Sosn Side Drilling Machine) for the financial support.

REFERENCES

UV Coated PVC Panel for Interior Wall Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Outdoor Printing Display Acrylic Sign Advertising Notice Board]

Brown, E. M; Thompson, C .J.,Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring., M.M.(1994),Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device.The Journal of the American Leather Chemists Association (JALCA)89(7)[Glass Salt & Pepper Mill/Grinder Glass Spice Jar]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[All Size Colored Tapping Hex Head Self Drill Screw Wood Screw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale 30W LED Down Lights with Budget Price. Analytical Biochemistry212,436-445[Low Frequency 3000W 24V 230V Inverter with Battery Charger]

Dalev, P.G. and Simeonova, L.S.(1992),Embossed Textured Wallpaper for Solvent Printing. Biotechnology letters 14,6, 531-534[Ss400 Galvanized Steel C Channel Steel Price]

Heidemann, E.(1997),Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray.Leder 48,(5),99-104[Wholesale White Color PE/ EVA 3D Foam Wallpaper Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)]

Large Size Outdoor Store Front Signs Advertising Billboard, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),4-14 Manual Staple Gun with Staples for Hand Tools.500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3). USP/SP[H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Smallest Portable Waste Transformer Oil Purifier Machine Zy-6: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Mobile Smartphone for Xiaomi 9 PRO]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fashion Open Pearl Ring for Women]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver]

Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Speed Automatic Packing Machine (GK-650 BA)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker]

Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud]

Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5000-5900MHz 4G Built-in Automotive Antenna, Part I.Glass Lined Reactor for Chemical Factory. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting]

OEM Metal Stamping Stainless Steel Parts with CNC Machining, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper.Supermarket Plastic T-Wire Basket Swing up Price Label Holder, 175-184[China All In One Travel Adapter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint]

Whitaker,J.R.(1963),1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine. Analytical Chemistry35(12), 1950-1953[PVC Shrink Sleeve Label for Drinking Water Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpmAuthor for correspondence