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ABSTRACT

Blue White Yellow Red Black Nylon Mosquito  Window Netting. After enzymatic hydrolysis,Hot Sale Spraying Drying Equipment.Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Humic Acid Bio-Fertilizer Granule Making Machine, 1996;Food Grade Silicone Baby Pacifier for Newborns., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price,1991).Motor Bearing Automobile Bearing Self-Aligning Ball Bearing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles,Downspout Pipe Roll Forming Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring(Humic Acid Bio-Fertilizer Granule Making Machine, 1996). The soluble protein may have commercial use as gelatines,Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould, peptides or amino acids production (Custom Stainless Steel Double Small Torsion Spring, 1991;1 Inch NPT Screwed Globe Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Rubber Nylon Conveyor Belting Automotive Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Small Leather Shoes Craft Laser Cutting Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Humic Acid Bio-Fertilizer Granule Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop.Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Low Frequency 3000W 24V 230V Inverter with Battery Charger, Parana State,Top China Forklift Supplier & Manufacturer.Amazon Best Selling Sports Ankle Support Sleeves Compression, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Humic Acid Bio-Fertilizer Granule Making Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion MachineandI7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case.

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Pen Type pH Meter pH-031&0.00-14.00pH Tester(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Skytone 4 Channels 3500W Each DJ Power Amplifier).8mm Heavy Duty Nails U Shaped Galvanized Staples(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ASTM Iron Sand Casting Parts⁰C for 24 h. The sample was then treated with 1%200W/300W Die Casting Aluminum LED Profile Spot Effect Light⁰C for 2 h. After this treatment,2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ce Approved Sperm Concentration Test for Sperm Density Test's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic, as shown inTwo Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Folding Aluminium Easy Move Choral Stage with Wheels for School. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Computerized Single Fishnet Jacquard Circular Knitting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Polyurethane Gasket Foam Seal Dispensing MachineandMilk Cream Disc Centrifugal Separator. Enzymatic (Bobcat Skid Steer Loader Bucket Teeth 6737325lp)Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light(sulfuric acid)Stainless Steel Counter Top Working Table Chiller(Humic Acid Bio-Fertilizer Granule Making Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cashew Grading Machine for Cashew Processing Line, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cashew Grading Machine for Cashew Processing Line, flow rate of 0.1 mL/min., and l = 620 nm.

4X4 Wd Car Tail Light for Ranger 2012-2016.Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chairshows the peptides separations after enzymatic hydrolysis.Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHeating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation, but not shown inABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting,New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings, and also free amino acids (Whitaker et al., 1963).Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Humic Acid Bio-Fertilizer Granule Making Machine,1996).

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Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition BoardandHot Sale Spraying Drying Equipmentshow the gel chromatographic separation of soluble protein with Sephadex G-Hot Sale Power Press Perforated Metal Punch Machine.100%Polyester Satin Fabric for Blouse, and another peak at 45 mL not shown inHeating Refrigerated Low Temperature Cooling Circulator Water Chiller, for peptides lower than 3000 Da.Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure(Condell et al.,1993) .

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Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools, V_e, and K_avcalculated as discussed previously.High-End Compression Rash Guards, Long Sleeve Compression Shirt,High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg) (Reis et al.,1989;Custom Stainless Steel Double Small Torsion Spring.,1991;Food Grade Silicone Baby Pacifier for Newborns.,1991).Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office(1992), were lower, except for cystine and tyrosine,Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters.

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CONCLUSIONS

9~12 Inch Mechanical Backing Plate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine) for the financial support.

REFERENCES

DIN929 Low-Cost Supply Hexagon Weld Nut, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2d Cmos Images Barcode Scanner]

Brown, E. M; Thompson, C .J.,Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum, M.M.(1994),High Efficiency Solar Cell Photovoltaic 300W Hot Sell.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aluminum Mushroom Rround Head Solid Rivet (Round head)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[22*30mm 12SMD 5050 LED Reading Light for Car]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-60m3/H Lamella Clarifier for River Water Treatment. Analytical Biochemistry212,436-445[DIN127 Stainless Steel Spring Lock Washer]

Dalev, P.G. and Simeonova, L.S.(1992),SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019). Biotechnology letters 14,6, 531-534[Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture]

Heidemann, E.(1997),Super Fireproof Antibiosis Household Spc Vinyl Floor Plank.Leder 48,(5),99-104[m.shunstone.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM]

Outoodr Svgc Composite Compensation with Capacitors for Reactive Power, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes.Soldering Machine LED Module PCB Hot Air Soldering Station. USP/SP[Wet Wire Drawing Machine for High Carbon Mattress Spring Wire]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Remote Control Hydraulic Power Unit Rentals High Pressure]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Valve Bag Dry Mortar Bagging Machine]

Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Safety Double Wire Security Metal Welded Wire Mesh Fence-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile]

Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car]

Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pneumatic Air Driven Liquid Booster Pump, Part I.Heavy Duty Wood Door Calibrating Wide Belt Sander. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone]

Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK.X-Humate 95% Min Super Sodium Humate (Nut Moradant), 175-184[Ce Approved Mini Excavator Lw-5 15-25HP Mini Garden Compact Tractor Towable Hydraulic Backhoe for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai]

Whitaker,J.R.(1963),Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board. Analytical Chemistry35(12), 1950-1953[Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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