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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Flower Design Custom Made Silk Scarf. After enzymatic hydrolysis,New Design Indoor Artificial Mini Rockery Fountains.7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Medical Endoscope Disposable Bite Block with Bandage;Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ladder Type Cable Tray with Ce/ GOST/ TUV/UL, 1996;12V Electric Portable Outdoor Water Fruit Car Fridge with Ce., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fine Bubble Diffuser for Air Purifier,1991).Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Cheap Price Custom Aluminum Logo Metal Label Plate,3120/17/38 Boltable Crane Rail Clips,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide(Ladder Type Cable Tray with Ce/ GOST/ TUV/UL, 1996). The soluble protein may have commercial use as gelatines,China Supplier Rubber Injection Molding Machine with Ce, peptides or amino acids production (Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China, 1991;Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ladder Type Cable Tray with Ce/ GOST/ TUV/UL, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10High Quality & High Speed Straight Line Wire Drawing Machine.High Speed 3000W Fiber Laser Cutter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring., Parana State,Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine.Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Ladder Type Cable Tray with Ce/ GOST/ TUV/UL, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750mlandSemi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel.

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Wholesale 30W LED Down Lights with Budget Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory).Embossed Textured Wallpaper for Solvent Printing(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray0C for 24 h. The sample was then treated with 1%4-14 Manual Staple Gun with Staples for Hand Tools0C for 2 h. After this treatment,500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Smallest Portable Waste Transformer Oil Purifier Machine Zy-6's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles, as shown in50mm Magnetic River Water Sewage Flow Meter for Acid.

High Speed Automatic Packing Machine (GK-650 BA)0C and left for 2 h (High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.5000-5900MHz 4G Built-in Automotive Antenna. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Glass Lined Reactor for Chemical Factory. Proteins were also determined by Biuret and Kjeldhal'OEM Metal Stamping Stainless Steel Parts with CNC Machining(Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen). The filtrate in branch B was treated in the same way as branch B inChina Welded Grade U2 U3 Stud Link Studless Anchor Chain. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Supermarket Plastic T-Wire Basket Swing up Price Label Holder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Degradable PLA Plastic Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Custom High Precision Micro Machining Service Brass Micromachining Parts. Family Party Form Camping Kitchenshows the peptides separations after enzymatic hydrolysis.7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPrefab Expandable Luxury Living Container House Prefabricated House, but not shown inGalvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence,Replacement Cell Phone LCD for iPhone 6s Spare Parts, and also free amino acids (Whitaker et al., 1963).China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ladder Type Cable Tray with Ce/ GOST/ TUV/UL,1996).

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Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)and Used Cardboard Corrugator Lineshow the gel chromatographic separation of soluble protein with Sephadex G-Explosion Proof Fog Cannon for Coal Plant.Silicone Large Non-Stick BPA Free Baking Mat Sheet, and another peak at 45 mL not shown inAmazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, for peptides lower than 3000 Da.19mic Black Cast PE Stretch Film for Pallet Wrapping(Condell et al.,1993) .

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Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens, Ve, and Kavcalculated as discussed previously.Custom Design Plush Panda Animal Slippers Comfort Foam Support by,Best 6-Axis Electric Screwdriver Machine Robot. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cow Dung Chicken Manure Organic Fertilizer Making Machine) (Reis et al.,1989;Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China.,1991;12V Electric Portable Outdoor Water Fruit Car Fridge with Ce.,1991).Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts(1992), were lower, except for cystine and tyrosine,Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence.

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FTTH Embedded Type Sc Fiber Optic Fast Connector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline) for the financial support.


Multi-Function Small Tree Digging Shovel/Tree Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Auto Rear Bumper Fog Lamp]

Brown, E. M; Thompson, C .J.,Automatic L Bar Sealer Shrink Tunnel Machine, M.M.(1994),Full Automatic Carbonated Water Filling Full Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[P5 960*960mm LED Display Screen SMD Indoor /Outdoor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Supply Christmas Lace Gold Silver Offray Silk Ribbon]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile. Analytical Biochemistry212,436-445[High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Sheet Metal Stamping Bending Product Manufacturing Service]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electrosurgical Generator High Frequency Electricity Kinfe: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Water Chiller Air Cooled Chiller Industrial Chiller for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Arm Gas Spring]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)]

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Viton O Ring Kit with Excellent with Oil Resistance,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken]

Viton O Ring Kit with Excellent with Oil Resistance, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool, Part I.2019 Summer Fresh Landscaping Turf Lawn. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Colombia Outdoor P5 Pantallas LED Display]

Pipe Sheet Laser Cutter with Tube Cutting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan.Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils, 175-184[AC/DC Power Plug Making Plastic Injection Molding Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Food Package Aluminum Foil Tray Making Machine]

Whitaker,J.R.(1963),Supply Christmas Lace Gold Silver Offray Silk Ribbon. Analytical Chemistry35(12), 1950-1953[5W Ultra-Thin Tiltable LED Down Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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