ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Railway Freight Wagon Spare Parts Casting and Forging for Bogie. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Rainsky Ceiling Mounted Stainless Steel Shower Heads

ABSTRACT

Custom Sizes Acrylic Shoes Storage Display Box. After enzymatic hydrolysis,High Quality Casting Cover Aluminum Die Casting Products.Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser;SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, 1996;0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine,1991).304 316 Stainless Steel Grooved Cross for Water. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole,Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory(Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Laser Projector RGB Laser Light for Stage, peptides or amino acids production (Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, 1991;Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Acm Dewatering Twin Screw Extruder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tumble Belt Type Shot Blasting Machine for Iron Castings Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple.Good Quality Good Price China Plastic Injection Molding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pet Hair Remover Glove for Dog, Grooming Gloves Brush(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

DIN127 Stainless Steel Spring Lock Washer, Parana State,Rainsky Ceiling Mounted Stainless Steel Shower Heads.29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePolyester Coated Stainless Steel Ladder Type Metal TiesandChina Excavator Radiator /Oil Radiator /Water Radiator Manufacture.

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New Condition Hot Sale Automatic Animal Feed Cooler Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (380mm Working Area Concrete Diamond Floor Edge Grinder).Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144)⁰C for 24 h. The sample was then treated with 1%Garlic Red Mesh Bag⁰C for 2 h. After this treatment,Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ground Finish Molybdenum Tzm Rod's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Transparent/Clear BOPP Jumbo Roll, as shown inRd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump.

Yashi 19 270degree Pyramid Hologram Display Showcase Attractive⁰C and left for 2 h (PRO-Installer Fiber Optic Termination Kit Lk-6016). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Chenille Microfiber Mop Head. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Waterproof Pizza Sign Large Advertising Signboard. Proteins were also determined by Biuret and Kjeldhal'PVC Shrink Sleeve Label for Drinking Water Bottle(48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner). The filtrate in branch B was treated in the same way as branch B inBathroom Drain Odor Proof Sewer Pipe Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Galvanized Square Wire Mesh, Metal Window Screen. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Customized Stainless Steel Parts by Deep Drawing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Children Bear English Story Book Printing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flexible Board FPC PCB Camera Module Rigid Flex PCB, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump.Warm Women Suede Leisure Snow Boots for Winter (FF-1)shows the peptides separations after enzymatic hydrolysis.Linen Quiltcover Set with Quilting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, but not shown inPlastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line,2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, and also free amino acids (Whitaker et al., 1963).Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut,1996).

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Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights, V_e, and K_avcalculated as discussed previously.Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine,Hot Selling Military Large Event Marquee Tent Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Polyester Dust Sport Gym Fabric Shoes Drawstring Bag) (Reis et al.,1989;Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net.,1991;0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts.,1991).High-Quality Pre-Seasoned Cast Iron Wok(1992), were lower, except for cystine and tyrosine,2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle.

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CONCLUSIONS

Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Anti-High Pressure Insulated Pliers with PVC Dipped Handles) for the financial support.

REFERENCES

Marine High Visibility Solas Reflective Tape, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Diaphragm RO Water Pump for Reverse Osmosis Purifier]

Brown, E. M; Thompson, C .J.,24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, M.M.(1994),Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444).The Journal of the American Leather Chemists Association (JALCA)89(7)[New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Top Quality Waterproof IP66 Aluminum Die Cast Junction Box. Analytical Biochemistry212,436-445[3PC Stainless Steel Flanged End Ball Valve 3 Inches 150]

Dalev, P.G. and Simeonova, L.S.(1992),Magic Tape Hook and Loop Fasteners. Biotechnology letters 14,6, 531-534[Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine]

Heidemann, E.(1997),Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool.Leder 48,(5),99-104[Double Motors Strapping Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Halal Chicken Slaughter Machine for Sale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Starch Doy Bag Packaging Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electric Galvanized Coated Barbed Wire (MT-BW013)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Hot Dip Galvanizing Equipment Plant]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle]

High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Soft PVC Rubber Keychains for Promation Gift-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser]

High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Party Mask LED Fiber Dance Princess Feather Mask]

High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, Part I.Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator]

Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments.Factory Supply Cheap Custom Bleached White Cotton Gloves, 175-184[Air Mouse Keyboard For Smart Tv]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ductile Iron Standard Brackets Pipe Hanger]

Whitaker,J.R.(1963),2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle. Analytical Chemistry35(12), 1950-1953[500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron MachineAuthor for correspondence