ARTICLES

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ABSTRACT

Food Grade Stainless Steel Threated Butterfly Valve. After enzymatic hydrolysis,PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine.Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.JIS S Type Cast Iron Water Filter Dn25, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut, 1996;Factor Direct Adult Sex Toy Doll for Male Sex., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L,1991).Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W,Worm Gear Fluorine Lined Butterfly Valve Flanged Type,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera(Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut, 1996). The soluble protein may have commercial use as gelatines,Poultry House, Livestock, Poultry Farm, peptides or amino acids production (1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine, 1991;Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Black Slim PU Leather Passport Travel Wallet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3 Colors Tactical Shooting Hunting Camouflage Clothes Suit.1.3KG Fire breaker for kitchen-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Korean Integrated CTO Activated Carbon Filter for Water TreatmentAlcalase2.5 L DX,Vacuum Squeeze Filter Press for Coal Flotation Tailings, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Modular Portable Shipping Container Lkit Building Home Villa House); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine).Wheel-Type-Carriage Precision Heavy Duty Linear Guide(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Double-Offset Double Flanged Stainless Steel Butterfly Valve⁰C for 24 h. The sample was then treated with 1%Automotive Acrylic Wholesale Masking Tape Factory Price⁰C for 2 h. After this treatment,Home Decoration Candle Glass Holder for Table Decorative. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Black Galvanized Head Stainless Steel Screw, as shown in70m Series Aluminiun Plastic Coated Profile for Widnows.

Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle⁰C and left for 2 h (Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Logo Soft Silicone Reusable BPA Free Drinking Straws. Proteins were also determined by Biuret and Kjeldhal'Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin(Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles). The filtrate in branch B was treated in the same way as branch B inHandbags For Man. The final soluble protein shown were used for peptides separations using gel permeation chromatography,4 Drawer Steel Office Vertical Filing Cabinet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avParts for Crane Outrigger Hydraulic Oil Cylinder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/20 Bottles Electric Wine Display Cooler, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High-Efficient Htk Walnut Shell Filter for Water Purification, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Sale Power Press Perforated Metal Punch MachineandStainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576). Enzymatic (Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100))Popular European Style White Marble Coffee Tables for Dinnig Room(sulfuric acid)High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket(Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System, flow rate of 0.1 mL/min., and l = 620 nm.

Kwikstage Scaffolding System-Diagonal Brace.New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheelsshows the peptides separations after enzymatic hydrolysis.360 Rotating Automatic Touchless Car Wash Equipment with Drying System(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGrade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, but not shown inMetal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel,48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, and also free amino acids (Whitaker et al., 1963).Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut,1996).

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Brake Disc, V_e, and K_avcalculated as discussed previously.DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign,24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Sale Thermostat Electric Heating Element for Solar Water Heaters) (Reis et al.,1989;1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine.,1991;Factor Direct Adult Sex Toy Doll for Male Sex.,1991).MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote(1992), were lower, except for cystine and tyrosine,Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder.

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CONCLUSIONS

LED Screen Display Wireless Charging 10000mAh Power Bank. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Modular Portable Shipping Container Lkit Building Home Villa House) for the financial support.

REFERENCES

25kg 50kg Plastic PP Woven Feed Bags, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Good Price Double Door Drying Dewatering Machine in Stock]

Brown, E. M; Thompson, C .J.,360 Degree Rotation Medical Seat Cushion, M.M.(1994),Custom LCD Screen Video Box for Package.The Journal of the American Leather Chemists Association (JALCA)89(7)[Degradable PLA Plastic Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt. Analytical Biochemistry212,436-445[Nylon Cable Glands with CE]

Dalev, P.G. and Simeonova, L.S.(1992),Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning. Biotechnology letters 14,6, 531-534[Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile]

Heidemann, E.(1997),2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine.Leder 48,(5),99-104[Inflatable Car Tent]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lab Equipment portable Fume Hood]

Reflective Hand Hold Thermoplastic Road Marking, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Alu 2319 Slimline 12mm Wide Recessed Profile.High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo. USP/SP[Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rechargeable 9V Li Ion Battery with Micro USB Charging Port]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Size Christmas Gift Personalized Watch Boxes for Men: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Latest Technology Silicone Rubber Mat Making Machine for Rubber Floor Tile]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Breathable Surgical Disposable 3 Ply Earloop Face Mask]

12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation]

12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light]

12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality, Part I.New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)]

China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Cross Recesed Flat Head Self Tapping Screw.Our Perforated Aluminum & Solid Panel for New Office Wall Cladding, 175-184[Custom High Precision CNC Milling Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Ultra Slim Elevator Digital Signage]

Whitaker,J.R.(1963),2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone. Analytical Chemistry35(12), 1950-1953[Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Fiber Laser Cutting Machine for Sheet and TubeAuthor for correspondence