ARTICLES

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^IICNPq/RHAE/biotechnology-Food Grade Stainless Steel Threated Butterfly Valve. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine

ABSTRACT

Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker. After enzymatic hydrolysis,Suction Function Handheld Cool and Heat Food Bowl for Baby.9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;JIS S Type Cast Iron Water Filter Dn25;Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factor Direct Adult Sex Toy Doll for Male Sex, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L, 1996;Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W,1991).Worm Gear Fluorine Lined Butterfly Valve Flanged Type. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera,Poultry House, Livestock, Poultry Farm,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine(C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L, 1996). The soluble protein may have commercial use as gelatines,Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2, peptides or amino acids production (Black Slim PU Leather Passport Travel Wallet, 1991;ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3 Colors Tactical Shooting Hunting Camouflage Clothes Suit.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1.3KG Fire breaker for kitchen. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign.Korean Integrated CTO Activated Carbon Filter for Water Treatment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vacuum Squeeze Filter Press for Coal Flotation Tailings(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow, Parana State,PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine.Wheel-Type-Carriage Precision Heavy Duty Linear Guide, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No RustandMineral Industry Magnet Ore Ball Milling Machine.

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Double-Offset Double Flanged Stainless Steel Butterfly Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Elastic Tape).Automotive Acrylic Wholesale Masking Tape Factory Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Home Decoration Candle Glass Holder for Table Decorative⁰C for 24 h. The sample was then treated with 1%Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars⁰C for 2 h. After this treatment,Wholesale Black Galvanized Head Stainless Steel Screw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair, as shown inFactory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts.

OEM Logo Soft Silicone Reusable BPA Free Drinking Straws⁰C and left for 2 h (LED Bulb Light with Remote Control RGB Christmas Bulb). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,4 Drawer Steel Office Vertical Filing Cabinet. Proteins were also determined by Biuret and Kjeldhal'12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car( Lgd4033). The filtrate in branch B was treated in the same way as branch B inNice Quality Coloring Printing Children Board Book Printing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Parts for Crane Outrigger Hydraulic Oil Cylinder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Boned Polyester Woven Faux Suede Sofa Fabric. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGood Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High-Efficient Htk Walnut Shell Filter for Water Purification⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Bluetooth Smart Watch with Heart Rate Monitor for Men 72087andPopular European Style White Marble Coffee Tables for Dinnig Room. Enzymatic (High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket)FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System(sulfuric acid)Kwikstage Scaffolding System-Diagonal Brace(C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%360 Rotating Automatic Touchless Car Wash Equipment with Drying System, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%360 Rotating Automatic Touchless Car Wash Equipment with Drying System, flow rate of 0.1 mL/min., and l = 620 nm.

48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner.Marine High Visibility Solas Reflective Tapeshows the peptides separations after enzymatic hydrolysis.Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMaleic Anhydride CAS 108-31-6, but not shown inHigh Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes,IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump, and also free amino acids (Whitaker et al., 1963).Sell Rubber / EVA / PE/PVC Foam Board. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L,1996).

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CONCLUSIONS

360 Degree Rotation Medical Seat Cushion. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Supplier of Excavator Spare Parts for Caterpillar Cat330) for the financial support.

REFERENCES

Custom LCD Screen Video Box for Package, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Top Quality 12 V 200ah High Temperature Solar Battery]

Brown, E. M; Thompson, C .J.,Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt, M.M.(1994),Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning.The Journal of the American Leather Chemists Association (JALCA)89(7)[Silicone Rubber Electronic Cable with High Flexibility]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Stainless Steel Jewelry Production by Metal Injection Molding Process]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine. Analytical Biochemistry212,436-445[6.6kw LiFePO4 Charger for Lithium Battery Pack]

Dalev, P.G. and Simeonova, L.S.(1992),Alu 2319 Slimline 12mm Wide Recessed Profile. Biotechnology letters 14,6, 531-534[Folding Aluminium Easy Move Choral Stage with Wheels for School]

Heidemann, E.(1997),High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo.Leder 48,(5),99-104[Compass Set]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Silicone Cake Mold Sinle Cupcake DIY]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chinese Prefab Building with Steel Frame Steel Structure]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant]

Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Home and Office PVC Vinyl Flooring Conductive Plank Flooring]

Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pet Laminate Aluminium Foil/Pet+Alu+PE]

Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX), Part I.Stainless Steel Cross Recesed Flat Head Self Tapping Screw. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure]

Our Perforated Aluminum & Solid Panel for New Office Wall Cladding, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone.Kids Trucker Hat, Kids Mesh Cap, 175-184[Expanded Metal Infill Panel Metal Mesh Custom Made]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing]

Whitaker,J.R.(1963),Wd190V Handheld Full Body Scanner Hand Held Metal Detector. Analytical Chemistry35(12), 1950-1953[Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminum Foil Heat Shield Screen Matting Adhesive BackedAuthor for correspondence