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ABSTRACT

Transport System China Manufacturer for Table Belt Conveyor. After enzymatic hydrolysis,Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal.Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Manufacturer Wallpaper House Decoration Wall Paper, 1996;Flexible Magnetic Rubber Sheet Soft Magnetic Tape., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass,1991).Electric Wine Bottle Opener K020. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen,China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)(China Manufacturer Wallpaper House Decoration Wall Paper, 1996). The soluble protein may have commercial use as gelatines,Spine Shape Landscaping Artificial Turf Grass, peptides or amino acids production (08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, 1991;3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Diesel Engine Type Zzs Sand Vibrating Screen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bluetooth Smart Watch with Heart Rate Monitor for Men 72087. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Manufacturer Wallpaper House Decoration Wall Paper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Trendy High Quality 925 Stering Silver Tennis Glod Charm.Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stand 2.5t Electric Pallet Truck(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Vertical Type Automatic Box Erector Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine).Air Cooled Scroll Type Water Chiller for Industrial Process(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China⁰C for 24 h. The sample was then treated with 1%Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090⁰C for 2 h. After this treatment,Rotary Vibrating Sieve Machine for Compost. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, as shown inStandard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price.

Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa⁰C and left for 2 h (Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube. Proteins were also determined by Biuret and Kjeldhal'Condoms/Sex Toys/Capsule Toy Vending Machine(Industrial Carbon Steel Wire Hose Hydraulic Pipe). The filtrate in branch B was treated in the same way as branch B inUSB 3.0 Cables. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor Type 40kgs Movable Ice Maker for Store Use(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electric 100W Three Wheels Toy Car for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2*2 4G Lte MIMO Antenna Rrouter/CPE, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter.10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Saleshows the peptides separations after enzymatic hydrolysis.China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, but not shown inFactory Price Diamond Wire Mesh Chain Link Fence,Cute Clear Fanny Pack Stadium Security PVC Waist Bag, and also free amino acids (Whitaker et al., 1963).Personalized Coffee Mug Cup with Custom Logo. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Manufacturer Wallpaper House Decoration Wall Paper,1996).

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Mango Drying Machine/Solar Fruit Drying MachineandDouble-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabricshow the gel chromatographic separation of soluble protein with Sephadex G-100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears.10 Pieces Customized Professional Makeup Brush, and another peak at 45 mL not shown in304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment, for peptides lower than 3000 Da.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone(Condell et al.,1993) .

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Electrophoresis Assembly Infilltration Tank for Effluent Treatment, V_e, and K_avcalculated as discussed previously.700mm RP HP UHP High Quality Graphite Electrode,Prefab Building Insulated Interior Concrete Partition Walls. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Best Selling Household Living Room Mat Chenille Carpets) (Reis et al.,1989;08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain.,1991;Flexible Magnetic Rubber Sheet Soft Magnetic Tape.,1991).Disk Filter System with Automatic Backwash Valves(1992), were lower, except for cystine and tyrosine,Eco-Friendly Recycling PS Foam Container Production Line.

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CONCLUSIONS

Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label) for the financial support.

REFERENCES

Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Printing Nonwoven Hook and Loop Tape for Diaper]

Brown, E. M; Thompson, C .J.,High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, M.M.(1994),Jacquard Football Fans Acrylic Sports Warmer Scarf.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wvrb Series Planetary Gearbox Used for Robot]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound. Analytical Biochemistry212,436-445[Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages]

Dalev, P.G. and Simeonova, L.S.(1992),Small Blinking Battery Operated Luminary Plastic LED Tea Lights. Biotechnology letters 14,6, 531-534[Free Sample! AC Power Contactor for Sale]

Heidemann, E.(1997),Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine.Leder 48,(5),99-104[Indoor Modular Flexible LED Display Screen for Advertising]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Orfs Male/Orfs Female Hose Fitting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Precision Plastic Mold Manufacturer in China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Suction Function Handheld Cool and Heat Food Bowl for Baby]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.ahtboat.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Shacman F3000 6X4 Truck Trailer Head]

Mango Drying Machine/Solar Fruit Drying Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mineral Industry Magnet Ore Ball Milling Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[WPC Decking Profile Making Machine]

Mango Drying Machine/Solar Fruit Drying Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet]

Mango Drying Machine/Solar Fruit Drying Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut, Part I.Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Good Quality NBR70 O-Ring with Reasonable Prices]

Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Blasting Cleaning Shot Peeling Machine Tumble Belt Type.B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine, 175-184[a-B-C-a Multi-Layer High Speed PPR Pipe Plastic Extrusion Machinery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security]

Whitaker,J.R.(1963),Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap. Analytical Chemistry35(12), 1950-1953[HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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