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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820). After enzymatic hydrolysis,High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box.Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner;160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, 1996;600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nbm High Quality Auto Part Factory Price 6pk2310,1991).Ultrasonic Automatic Face Mask Blank Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manufacturer of Soda Ash Dense 99 2% Price,Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Headband Big Headphone EVA Bag Tool Case Specialized Storage Box(AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, 1996). The soluble protein may have commercial use as gelatines,Chicken Slaughtering Machine for Sale, peptides or amino acids production (750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish, 1991;Hf Vacuum Dryer Machine with Advanced High Frequency Technology, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 102019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women.Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Oak Laminate Grey Floating Cork Vinyl Plank Flooring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Electrical 4W AC Synchronous Gear Motor for Engraving Machine, Parana State,CE Approved Manual Heat Press Machine.Custom Car Seat Belt Buckle Mold Maker, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm FencingandEco-Friendly Recycling PS Foam Container Production Line.

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DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper ( Strong Flexibility Spoke Wire).Exfoliating Shower Hemp Bath Sponge Pad(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry0C for 24 h. The sample was then treated with 1%Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)0C for 2 h. After this treatment,Sequins Butterfly Handmade Beads Embroidery Patch. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier, as shown in165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man.

Adjustable Aluminum Solar Mounting System0C and left for 2 h (1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Wireless Cheapest Android 2D Barcode Scanner. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Factory Price Diamond Wire Mesh Chain Link Fence. Proteins were also determined by Biuret and Kjeldhal'Electric Heating Mantle 5L(Brake Disc). The filtrate in branch B was treated in the same way as branch B inCathode Graphite Plate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavOutdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/FRP GRP Fiber Reinforced Plastic Fiberglass Gratings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Home Use Standing Modern Floor Lamp (KAF6108).High Quality Customized Aluminum Foil Shisha Sheetshows the peptides separations after enzymatic hydrolysis.Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Mini LED Flood Light SMD Flood Light 50W, but not shown inStainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer,1.75mm 3mm ABS PLA PC 3D Printer Filament Machine, and also free amino acids (Whitaker et al., 1963).Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m,1996).

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Modern Vertica Parking Garage System. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


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Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[qidi-tec.com]

Brown, E. M; Thompson, C .J.,Galfan Wire (Aluminium-Zinc Alloy coated), M.M.(1994),1000base Single Mode 1310nm LC SFP Module 20km 1.25g.The Journal of the American Leather Chemists Association (JALCA)89(7)[10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Steel Bar Peeling Lathe Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant). Analytical Biochemistry212,436-445[Pipe Sheet Laser Cutter with Tube Cutting]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LED Screen Display Wireless Charging 10000mAh Power Bank]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pearls Flake 99% Factory Naoh Caustic Soda]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Manufacture Commercial Use Spice Grinder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Barrier Film For Packaging]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Baby Feeding Chair Plastic Chair]

Whitaker,J.R.(1963),1.5/2 power 50Hz ZN SERIES Vibrator motor. Analytical Chemistry35(12), 1950-1953[Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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