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ABSTRACT

Custom Design Plush Panda Animal Slippers Comfort Foam Support by. After enzymatic hydrolysis,Best 6-Axis Electric Screwdriver Machine Robot.Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multi-Function Small Tree Digging Shovel/Tree Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic L Bar Sealer Shrink Tunnel Machine, 1996;Full Automatic Carbonated Water Filling Full Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile,1991).Superior Watertightness JS Polymer Cement Waterproof Coating for Basement. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PS 200 Upper Discharge Centrifuge,3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures(Automatic L Bar Sealer Shrink Tunnel Machine, 1996). The soluble protein may have commercial use as gelatines,Electrosurgical Generator High Frequency Electricity Kinfe, peptides or amino acids production (Viton O Ring Kit with Excellent with Oil Resistance, 1991;One Step HBV 5 in 1 Rapid Test, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 Summer Fresh Landscaping Turf Lawn.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pipe Sheet Laser Cutter with Tube Cutting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic L Bar Sealer Shrink Tunnel Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan.Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Supply Christmas Lace Gold Silver Offray Silk Ribbon(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)).4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower⁰C for 24 h. The sample was then treated with 1%Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder⁰C for 2 h. After this treatment,10g Non-Woven Fabric Silica Gel Desiccant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Soosan Excavator Hydraulic Hammer for Construction Demolition's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover, as shown inHigh Speed Automatic Packing Machine (GK-650 BA).

Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine⁰C and left for 2 h (Electric Paint Sprayer Pump). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950. Proteins were also determined by Biuret and Kjeldhal'Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys(Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar). The filtrate in branch B was treated in the same way as branch B inBest Price Plywood Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Cacao Bean Grinder Cocoa Butter Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNh4cl White Granular Agricultural Grade Ammonium Chloride(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Spooling Pet Polyester Mylar Film for Cable Material, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Best Design and Nice Price All in One Solar Street Light.Shanghai China Professional Plastic Injection Moulding Plastic Mold Makershows the peptides separations after enzymatic hydrolysis.Customized Large Ss Stamping/ Bending Sheet Metal Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inManufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale, but not shown in150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels,High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet, and also free amino acids (Whitaker et al., 1963).UV Laser Marking Machine (with Air Cooling System). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic L Bar Sealer Shrink Tunnel Machine,1996).

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CONCLUSIONS

Iron Welded Gabion Basket Retaining Wall. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light) for the financial support.

REFERENCES

Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plate and Shell Heat Exchanger for Cooling Oil or Air]

Brown, E. M; Thompson, C .J.,Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale, M.M.(1994),Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-FTTX Solution Provider Fiber Optical 12cores MPO Cassette. Analytical Biochemistry212,436-445[Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring]

Dalev, P.G. and Simeonova, L.S.(1992),Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer. Biotechnology letters 14,6, 531-534[304 &314 Stainless Steel Seamless Bending Tube]

Heidemann, E.(1997),Aluminum Height Adjustable Cane.Leder 48,(5),99-104[Perfume Bottle Square 50ml Glass Fragrance Reed Diffuser Bottle for Room]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient]

Silicone Large Non-Stick BPA Free Baking Mat Sheet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board.Large Batches Equipoise Light Yellow Liquids. USP/SP[Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality & High Speed Straight Line Wire Drawing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[125kHz RFID Hotel Key Card with T5577 Chip]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbon Steel Bending Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Black Galvanized Head Stainless Steel Screw]

Wholesale Custom Football Net Soccer Goal Net for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard]

Wholesale Custom Football Net Soccer Goal Net for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Spine Shape Landscaping Artificial Turf Grass]

Wholesale Custom Football Net Soccer Goal Net for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Load 1600kg Small Machine Room Bed Elevator, Part I.Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sanjing PVC Lining Layflat Fire Hose]

Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Invention 2017 Male & Female Stainless Steel Quick Coupling.Driving System for Hybrid Stepper Motor Dm860A, 175-184[High Quality 9W IP20 Indoor Round Deep Anti-Glare Recessed LED Downlight]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Metal Soft Enamel Souvenir Metal Medal Sport Medallions]

Whitaker,J.R.(1963),Atf-805 Auto Transmission System Flushing Machine with CE/SGS. Analytical Chemistry35(12), 1950-1953[Classic Black and White Plaid Sling Bag for Women]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Black DiamondAuthor for correspondence