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ABSTRACT

Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. After enzymatic hydrolysis,Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery.Printed Wristband Cotton Wristband RFID Woven Wristband. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Real Leather Wallet Mens Designer Leather Wallets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ASTM A240 321 Stainless Steel Sheet Price Per Kg, 1996;V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Promotional Plain Silver Empty Hinged Rectangular Tin Box,1991).Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer,Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale(ASTM A240 321 Stainless Steel Sheet Price Per Kg, 1996). The soluble protein may have commercial use as gelatines,Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), peptides or amino acids production (Cantilever Oxygen Plasma Cutter 80, 1991;Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Fiber Laser Cutting Machine for Sheet and Tube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ASTM A240 321 Stainless Steel Sheet Price Per Kg, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole.Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bio Media(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner).Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Platform Trolley with 150kg Capacity, 4 Castor Wheel⁰C for 24 h. The sample was then treated with 1%Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine⁰C for 2 h. After this treatment,LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)All Size Colored Tapping Hex Head Self Drill Screw Wood Screw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pre-Engineered Cage Broiler Poultry Farm Building, as shown inCNC Aluminum Sheet Press Brake.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Powerful Anti-Stress, Strong Flexibility Spoke Wire. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avModern American Style Beech Solid Wood Kitchen Cabinet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Machining Metal/Aluminum/Brass Auto Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Surprise! Range Hood Free! ! ! Juice Cart, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Chicken Layer Cage Poultry Cage Chicken Cages, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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En1092-1 05 Pn16 Blind Flat Face Flange.Swimming Pool Water Disinfect Ozone Generatorshows the peptides separations after enzymatic hydrolysis.Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing, but not shown in7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input,Peristaltic Pump with Connecting The High Speed Tubular Separator, and also free amino acids (Whitaker et al., 1963).High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (ASTM A240 321 Stainless Steel Sheet Price Per Kg,1996).

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CONCLUSIONS

High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (BS 5163 Ductile Iron Rising Stem Gate Valve) for the financial support.

REFERENCES

12ml Round Shape Empty Clear Nail Polish Glass Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Finks 41 Inch Spruce Zebrawood Folk & Acoustic Guitar Popular Guitar]

Brown, E. M; Thompson, C .J.,M4X10 Round Head Core Pulling Aluminum Nail, M.M.(1994),Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cracker Multi Rows Trayless Packaging Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Skytone 4 Channels 3500W Each DJ Power Amplifier. Analytical Biochemistry212,436-445[Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System]

Dalev, P.G. and Simeonova, L.S.(1992),Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price. Biotechnology letters 14,6, 531-534[Bottom Pushing for Fire Extinguisher Hot Spinning Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[80GSM C2s Coated Glossy/Matt Art Paper for Printing]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Double Wine Carrier Bag Gift Box Tote: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Cross Recesed Flat Head Self Tapping Screw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale Nylon Double Braid Mooring Rope]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gen 2+ or 3 Day/Night Vision Scope]

(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stop Dog From Jumping or Climbing Your Chain Link Fence.]

(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt]

(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flexible PVC Granules for Cable, Wire and Insulation, Part I.7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[0.1mm Tungsten Carbide 2f Single Flute Micro End Mill]

Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Brass Pump Spare Parts for Circulating Pump.Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes, 175-184[Family House Resort Villa with Assembled Container Module]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould]

Whitaker,J.R.(1963),Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi. Analytical Chemistry35(12), 1950-1953[High Quality Army Captain Navy Blue Color Hat for Army Tender]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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