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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine. After enzymatic hydrolysis,Vertical Automatic Granule Bag Packing Machine.Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures;4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Small Manufacturing Tht/ Ai Double Wave Soldering Machine, 1996;Manual Sealing Machine for Instant Noodles Paper Soup Bowls., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck,1991).Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion,Corrugated Steel Sheet Corrugated Steel Roofing Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Factory Queen Mattress with Visco Memory Foam and Latex(Small Manufacturing Tht/ Ai Double Wave Soldering Machine, 1996). The soluble protein may have commercial use as gelatines,High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, peptides or amino acids production (Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, 1991;Customized Hot Forging Agricultural Spare Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;16mm 12 Strand UHMWPE Synthetic Fiber Rope., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Vertical Type Caseformer Machine WJ-LKX-15. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Small Manufacturing Tht/ Ai Double Wave Soldering Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine.Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:0.1mm Tungsten Carbide 2f Single Flute Micro End Mill(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Agriculture Large Span Commerical Steel Frame GreenhouseAlcalase2.5 L DX,Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Ss400 Corner Metal Sheet Bar Galvanized Angle Iron(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine, Parana State,Six Stations Pen Press Machine.Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Small Manufacturing Tht/ Ai Double Wave Soldering Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the1650mm*80mm Stable Power Laser TubeandTablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835.

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Chinese Jbcz Handy Operation Threading Machine and Coupler(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Bobby Flay Websites Restaurant Martha Stewart Designer Tray).Promotional Reusable Foldable PP Non Woven Shopping Bag(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board0C for 24 h. The sample was then treated with 1%Factory Price Printing Doy Pack Bag0C for 2 h. After this treatment,Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price, as shown inExport Grade Citric Acid Monohydrate as Detergent.

High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR170C and left for 2 h (1325 CNC Engraving Router). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Engine Parts Rubber High Quality Timing Belt 111ru25. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Wholesale Interior Metal 3D Bee Garden Ornament Decoration. Proteins were also determined by Biuret and Kjeldhal'Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket(Custom LCD Screen Video Box for Package). The filtrate in branch B was treated in the same way as branch B inWater Filter System Lamella Clarifier for River Water Treatment System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Disposable Non-Woven Dry Gloves Wipes Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavWaterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fish Scales Texture Fashion Leather for Shoe (S312100GH)0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Diamond Segments 2000mm Diameter Granite Cutting Blade, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Produce N2o for Medical Gas Equipments, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


50kg Unmanned Two-Way Magnetic Tape Guided AgvandHot Selling Furring Channel Fittings Suspended Ceiling Accessories. Enzymatic (90m3 Per Hour Mobile Concrete Pump)China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter(sulfuric acid)FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node(Small Manufacturing Tht/ Ai Double Wave Soldering Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%All Weather Rattan Handwoven Swing Outdoor Home, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%All Weather Rattan Handwoven Swing Outdoor Home, flow rate of 0.1 mL/min., and l = 620 nm.

Pond Self Priming Booster suction  Water Pump with Pressure Switch.Boned Polyester Woven Faux Suede Sofa Fabricshows the peptides separations after enzymatic hydrolysis.Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, but not shown inWholesale China Merchandise 12V Horizontal Mini Camping Fridge,Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace, and also free amino acids (Whitaker et al., 1963).for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Small Manufacturing Tht/ Ai Double Wave Soldering Machine,1996).

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Our Perforated Aluminum & Solid Panel for New Office Wall CladdingandEasy Foldable Kids Walking Chair Toys Baby Walkershow the gel chromatographic separation of soluble protein with Sephadex G-100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree.School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), and another peak at 45 mL not shown in220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist, for peptides lower than 3000 Da.Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle(Condell et al.,1993) .

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Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation, Ve, and Kavcalculated as discussed previously.0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine,125kHz RFID Hotel Key Card with T5577 Chip. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal) (Reis et al.,1989;Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring.,1991;Manual Sealing Machine for Instant Noodles Paper Soup Bowls.,1991).USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug(1992), were lower, except for cystine and tyrosine,Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag.

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Crop Fertilizer Agriculture Nh4cl Ammonium Chloride. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags) for the financial support.


Wholesale High Pressure 80L Nitrogen Gas Cylinder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Kids Medal Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,10mm Red Green Yellow Warning China Supplier Signal Lamp, M.M.(1994),Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale Thermostat Electric Heating Element for Solar Water Heaters. Analytical Biochemistry212,436-445[Square Plastic PP Geocomposite Drainage Blind Ditch/Drain]

Dalev, P.G. and Simeonova, L.S.(1992),Phillip Countersunk Head Screw Hot DIP Galvanized. Biotechnology letters 14,6, 531-534[Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools]

Heidemann, E.(1997),Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse.Leder 48,(5),99-104[Car For Children]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dual Power Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3W LED Explosion Proof Emergency Exit Light]

Ductile Iron Standard Brackets Pipe Hanger, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Long Sleeve Compression Shirt]

Ductile Iron Standard Brackets Pipe Hanger,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Arrival Wooden Kids Cleaning Set Toy for Education W10d251]

Ductile Iron Standard Brackets Pipe Hanger, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, Part I.5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[FRP GRP Fiber Reinforced Plastic Fiberglass Gratings]

High Quality Sheet Metal Bending Roll Forming Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Different Size Glass Test Tubes, High Quality Laboratory Test Tubes.Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine, 175-184[Piston Lift Gas Spring Pneumatic Hydraulic for Massage Table]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Gym Fitness Exercise Sporting Body Building Bicep Press]

Whitaker,J.R.(1963),Frame Type Rubber Hydraulic Press Machine. Analytical Chemistry35(12), 1950-1953[1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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