ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-ASME/ASTM Butt-Welding Stainless Steel Plate Flange. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank

ABSTRACT

Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings. After enzymatic hydrolysis,Polyester / Polypropylene Woven Geotextile for Road Construction.Granite Stone Jaw Crusher of Mining Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor;Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag, 1996;Wholesale All Kinds Cheap Plush Animal Baby Slippers., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car,1991).Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine,New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale(Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag, 1996). The soluble protein may have commercial use as gelatines,Taiding Custom Tank Sheet Metal Fabrication, peptides or amino acids production (Airtight Candy Bin with Scoop, 1991;Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3 Layer Round Tea Tin with Air Tight Lid.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China PA System Power Amplifier with Enough Rated Power. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Export Grade Citric Acid Monohydrate as Detergent.Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Familu Use Mini Small Pig Feed Pellet Mill(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine⁰C and left for 2 h (Felt Belt for Textile Compactor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Q32 Series Tumble Belt/Crawler Shot Blasting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Galvanized Bar Grating. Proteins were also determined by Biuret and Kjeldhal'High Quality Custom Self Adhesive Leather Patch for Jackets(100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve). The filtrate in branch B was treated in the same way as branch B inStainless Steel Stamping Part. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%380mm Working Area Concrete Diamond Floor Edge Grinder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAngle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Discussion and Interpretation System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Frozen Soybean in Pod, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Zipper Fabric Travel Passport Wallet Organiser.Wall Washer Light Building Decoration Landscape LED IP65 Linear Lightshows the peptides separations after enzymatic hydrolysis.Hot Sale Instax Mini Camera Korea Style Photo Album(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inmarble Makeup Brush Set for Travel in 20PCS, but not shown inRound Casting Iron Trench Cover Manhole Cover Frame,Aluminum Low Profile Adjustable Bracket, and also free amino acids (Whitaker et al., 1963).Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag,1996).

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Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle, V_e, and K_avcalculated as discussed previously.Plastic Injection Molding for Auto Parts,Round Type Twin Screw Extruder Barrel Manufacturer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Design Indoor Artificial Mini Rockery Fountains) (Reis et al.,1989;Airtight Candy Bin with Scoop.,1991;Wholesale All Kinds Cheap Plush Animal Baby Slippers.,1991).ASA-UPVC Anti-Corrosive Composite Roof Tile(1992), were lower, except for cystine and tyrosine,Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System.

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CONCLUSIONS

Sexy Mesh Transparent Female Running Tank Top with Hood Custom. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, M.M.(1994),Home and Office PVC Vinyl Flooring Conductive Plank Flooring.The Journal of the American Leather Chemists Association (JALCA)89(7)[Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ss400 Corner Metal Sheet Bar Galvanized Angle Iron]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Precision Engineering Parts Bearing Support Unit for Ball Screw. Analytical Biochemistry212,436-445[Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole]

Dalev, P.G. and Simeonova, L.S.(1992),LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection. Biotechnology letters 14,6, 531-534[Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse]

Heidemann, E.(1997),165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man.Leder 48,(5),99-104[Cbd Vape Oil]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Type Price Prefabricated Roof Tile in Sudan]

Injector Pump Calibration Test Bench, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Silicone Cake Mold Sinle Cupcake DIY.Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting. USP/SP[V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Aluminium Windows Thermal Break China Supplier]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dn400--3000 New Product Plastic HDPE Irrigation Drain Pipe High Pressure Drinking Water HDPE Carat Pipe Krah Spiral Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker]

Cross Stripe Sleeveless Yoga Wear, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Construction & Decoration ERW Square Steel Tube Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Super Fireproof Antibiosis Household Spc Vinyl Floor Plank]

Cross Stripe Sleeveless Yoga Wear,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122]

Cross Stripe Sleeveless Yoga Wear, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh, Part I.Black Steel ASTM A192 Seamless Pipe Sch120. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow]

China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine.BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, 175-184[Pressure PP Plate Mechanical Machinery Laboratory Palm Oil Sand Filter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making]

Whitaker,J.R.(1963),Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel. Analytical Chemistry35(12), 1950-1953[DIN Standard Gratings/Gavlanized Steel Grating]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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