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ABSTRACT

9~12 Inch Mechanical Backing Plate. After enzymatic hydrolysis,DIN929 Low-Cost Supply Hexagon Weld Nut.Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, 1996;High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Soldering Machine LED Module PCB Hot Air Soldering Station,1991).220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W,High Safety Double Wire Security Metal Welded Wire Mesh Fence,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pneumatic Air Driven Liquid Booster Pump(Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, 1996). The soluble protein may have commercial use as gelatines,Heavy Duty Wood Door Calibrating Wide Belt Sander, peptides or amino acids production (Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X, 1991;Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;X-Humate 95% Min Super Sodium Humate (Nut Moradant)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Women Summer Swimsuit Chest Zip Bathing Suit. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hemp Seed Oil Body Lotion Moisturizes The Skin.SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Galvanized Hexagonal Wire Mesh Gabion / Gabion Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Supermarket Plastic T-Wire Basket Swing up Price Label Holder).10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Must 4kw 2HP Pump Power Inverter Ags Bts Available⁰C for 24 h. The sample was then treated with 1%Hot Sale Small Steel Silo for Poultry Feed⁰C for 2 h. After this treatment,Good Price Nasal Paste Packaging Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame, as shown inSmall Frame Hydraulic Press Machine for Bending Price.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMs Gi Pipe Dn15~Dn400 Gi Pipe List(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Design Underwear for Men Cotton Mens Boxer Briefs, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Automatic Chicken Nipple Drinker System. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box) for the financial support.

REFERENCES

Industrial Hydraulic Ice Grape Press Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Curved Nail Tips]

Brown, E. M; Thompson, C .J.,Common Rail Diesel Injector Nozzle Dlla153p977, M.M.(1994),Betaine 98% Betaina Feed Grade.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hairdressing Equipment Takara Belmont Barber Chair High Quality]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Glass Salt & Pepper Mill/Grinder Glass Spice Jar. Analytical Biochemistry212,436-445[Sand Castings by Gray Iron Material]

Dalev, P.G. and Simeonova, L.S.(1992),Outsole Trimming Machine. Biotechnology letters 14,6, 531-534[Must 4kw 2HP Pump Power Inverter Ags Bts Available]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Top10 1080P H. 264 P2p CCTV WiFi IP Camera]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Injection Molding for Auto Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PVC Profile with Net Making Machine Used for Window Sealing Profile]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pink Backpack]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle]

PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder]

PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap]

PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump, Part I.ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Square Premium Glass Perfume Bottle 100ml]

Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen.DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw, 175-184[Aluminum Film PP Nonwoven Reflective Alu Film Nonwoven]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Price Printing Doy Pack Bag]

Whitaker,J.R.(1963),Auto Two Piece Folder Gluer for Corrugated Box. Analytical Chemistry35(12), 1950-1953[Xjm Series Rubber Extruding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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