ARTICLES

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^IICNPq/RHAE/biotechnology-Crop Fertilizer Agriculture Nh4cl Ammonium Chloride. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wholesale High Pressure 80L Nitrogen Gas Cylinder

ABSTRACT

10mm Red Green Yellow Warning China Supplier Signal Lamp. After enzymatic hydrolysis,Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor.Hot Sale Thermostat Electric Heating Element for Solar Water Heaters. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Phillip Countersunk Head Screw Hot DIP Galvanized;Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heat Shrink Automatic Flow Wrapping Packing Machine, 1996;Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ductile Iron Standard Brackets Pipe Hanger,1991).Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper,5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Sheet Metal Bending Roll Forming Machine(Heat Shrink Automatic Flow Wrapping Packing Machine, 1996). The soluble protein may have commercial use as gelatines,Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, peptides or amino acids production (Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine, 1991;Frame Type Rubber Hydraulic Press Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Kitchen Metal Dish Storage Rack (JP-SC984C)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-AVR Se350 Automatic Voltage Regulator for Marathon Alternator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heat Shrink Automatic Flow Wrapping Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage.Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Strength Rigid PVC Plastic Foam Board for FRP(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar).Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ADSS Newest Ultra Therapy Skin Tightening 3D Hifu⁰C for 24 h. The sample was then treated with 1%Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap⁰C for 2 h. After this treatment,Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Ceramic Chrome Hybrid Bearing Oil's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, as shown inAntique Gold/Copper Coin/Bright Gold/Copper London Ride Medal.

CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder⁰C and left for 2 h (CNC Machining 6601 Aluminum Parts with Anodized Automoblile). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Digger Arm Small Wheel Loader (HQ908) with CE. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer. Proteins were also determined by Biuret and Kjeldhal'Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output(Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll). The filtrate in branch B was treated in the same way as branch B inwww.clin-ele.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Water Based Emulsion Paint Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cnm Single Strand Anchor Coupler. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sterile Dsiposable Syringe for Dental 12ml, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Foldable Bed Military Cot for Camping, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears.Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)shows the peptides separations after enzymatic hydrolysis.1 Ton Chain Pulley Block with Geared Trolley(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inQuality Shaper Machine Bc6085 Shapping Machine, but not shown inEbay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery,Powder Coating Polyester Resin with Factory Price, and also free amino acids (Whitaker et al., 1963).Auto Plastic Part Hardware Plastic Injection Mold. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Heat Shrink Automatic Flow Wrapping Packing Machine,1996).

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Ozonated Water for Vegetables and Fruits Washing Ozone GeneratorandBlue White Yellow Red Black Nylon Mosquito  Window Nettingshow the gel chromatographic separation of soluble protein with Sephadex G-Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.IEC 62196-2 Type 2 EV Charging 32A Male Plug, and another peak at 45 mL not shown inConcrete Aluminum Formwork System Aluminium Formwork, for peptides lower than 3000 Da.PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter(Condell et al.,1993) .

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CONCLUSIONS

Outdoor Concert Stage Equipment Aluminum Stage Portable Stage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve) for the financial support.

REFERENCES

Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cooler Box]

Brown, E. M; Thompson, C .J.,22*30mm 12SMD 5050 LED Reading Light for Car, M.M.(1994),SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Highly Stable Performance Disposable Blood Lancet with Cheap Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Galvanized Bar Grating]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness. Analytical Biochemistry212,436-445[2019 Summer Fresh Landscaping Turf Lawn]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor Cheap Portable Stage Aluminum Truss Stage. Biotechnology letters 14,6, 531-534[Wholesale Custom 100% Cotton Boys T-Shirt with Print]

Heidemann, E.(1997),One Arm Motorized Multifunction Hospital Equipment for China Supply.Leder 48,(5),99-104[2816 Шариковый винт]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[T6s 86 PCD Core Bit for Geotechnical Drilling]

Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts.P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display. USP/SP[190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Flooded Type Water Cooled Screw Chiller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.zohofurniture.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape]

Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Size Christmas Gift Personalized Watch Boxes for Men]

Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines]

Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Printed Wristband Cotton Wristband RFID Woven Wristband, Part I.High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automotive Perforated Metal Stamping Part for Furniture]

Dolphin Designed Handheld Massager (LC-889), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Real Leather Wallet Mens Designer Leather Wallets.ASTM A240 321 Stainless Steel Sheet Price Per Kg, 175-184[Iaaf Certificate Athletic Running Track]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fancy Veneer Plywood. Natural Veneer Plywood for Furniture]

Whitaker,J.R.(1963),V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor. Analytical Chemistry35(12), 1950-1953[Best Design and Nice Price All in One Solar Street Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Durable Comfortable Rubber Non Slip Mouse Pad Wrist RestAuthor for correspondence