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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Clear PVC Sheet with Coating for Card. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Classic Black and White Plaid Sling Bag for Women


24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable. After enzymatic hydrolysis,10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor.Free Sample E Liquid/E-Juice for Nicotine Vaping. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft;100% Acrylic Knitted Sports Woven Scarf; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Prefab Steel Structure Shed for Storage Warehouse, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, 1996;Gen 2+ or 3 Day/Night Vision Scope., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved,1991).3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1650mm*80mm Stable Power Laser Tube,FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Lead Ingot 99.994 Reasonable Price(Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, 1996). The soluble protein may have commercial use as gelatines,Micro-Combustion Tube, Test Tube (HK-224), peptides or amino acids production (Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen, 1991;OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3W LED Explosion Proof Emergency Exit Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stationery PP Clipboard With 7 Inch Scale Ruler. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box.Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVC Cross Joint Pipe Fittings Equal Pipe Cross(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


3PC Stainless Steel Flanged End Ball Valve 3 Inches 150Alcalase2.5 L DX,Ysdcnc Manual Sheet Metal Plate Bending Roller Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Betaine Anhydrous 98% Food Grade Good Quality Competitive Price); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Bearing Stand Type Cam Follower, Parana State,Classic Black and White Plaid Sling Bag for Women.Hot Sale Electric LED light Aroma Diffuser, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndependent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass BoardandAlu 2319 Slimline 12mm Wide Recessed Profile.

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Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (AAC Block Production Line / AAC Equipment Manufacturing/ Machinery).DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps0C for 24 h. The sample was then treated with 1%7PCS RGBW LED Plastic PAR Light Wall Washer0C for 2 h. After this treatment,750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Kindergarten Playground Preschool Kids Plastic Slide and Swing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, as shown inNew Design Indoor Artificial Mini Rockery Fountains.

Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/12000C and left for 2 h (Galvanized Square Washer with Timber Constructions). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Railway Freight Wagon Spare Parts Casting and Forging for Bogie. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Rainsky Ceiling Mounted Stainless Steel Shower Heads. Proteins were also determined by Biuret and Kjeldhal'Custom Sizes Acrylic Shoes Storage Display Box(Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine). The filtrate in branch B was treated in the same way as branch B inFleece Balaclava Hat Hooded Neck Warmer Winter Sports Face Mask Gray Black. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Casting Cover Aluminum Die Casting Products.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, pH 3.25., mobile phase B: buffer solution of 0.01 mol/0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in ChinaandCommercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine. Enzymatic (304 316 Stainless Steel Grooved Cross for Water)Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole(sulfuric acid)Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home(Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Sale Laser Projector RGB Laser Light for Stage.Ss400 Corner Metal Sheet Bar Galvanized Angle Ironshows the peptides separations after enzymatic hydrolysis.Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole, but not shown inWiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse,Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, and also free amino acids (Whitaker et al., 1963).Acm Dewatering Twin Screw Extruder. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture,1996).

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V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/OutdoorandButt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9show the gel chromatographic separation of soluble protein with Sephadex G-Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820).Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, and another peak at 45 mL not shown inHigh Quality Aluminium Windows Thermal Break China Supplier, for peptides lower than 3000 Da.Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple(Condell et al.,1993) .

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Good Quality Good Price China Plastic Injection Molding Machine, Ve, and Kavcalculated as discussed previously.Pet Hair Remover Glove for Dog, Grooming Gloves Brush,Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Super Fireproof Antibiosis Household Spc Vinyl Floor Plank) (Reis et al.,1989;Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen.,1991;Gen 2+ or 3 Day/Night Vision Scope.,1991).Alumina Balls for Hydogen Peroxide(1992), were lower, except for cystine and tyrosine,Anti-High Pressure Insulated Pliers with PVC Dipped Handles.

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Hot Selling Electronical Blue Pet Plastic Release Film Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Betaine Anhydrous 98% Food Grade Good Quality Competitive Price) for the financial support.


DIN127 Stainless Steel Spring Lock Washer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[]

Brown, E. M; Thompson, C .J.,29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom, M.M.(1994),New Condition Hot Sale Automatic Animal Feed Cooler Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[DIN Standard Gratings/Gavlanized Steel Grating]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System. Analytical Biochemistry212,436-445[Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin]

Dalev, P.G. and Simeonova, L.S.(1992),Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144). Biotechnology letters 14,6, 531-534[En1092-1 05 Pn16 Blind Flat Face Flange]

Heidemann, E.(1997),Garlic Red Mesh Bag.Leder 48,(5),99-104[Picosecond Tattoo Removal RF Dpl Hair Removal for Pigmentation Treatment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing]

Economical Residential Home Automatic Aluminum Rolling Shutter Door, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder.Ground Finish Molybdenum Tzm Rod. USP/SP[Customized Hot Forging Agricultural Spare Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ladder Type Cable Tray with Ce/ GOST/ TUV/UL]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Transparent/Clear BOPP Jumbo Roll: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Good Medical 4D Fetal Imaging Color Doppler Ultrasound Diagnostic System]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module]

Yashi 19 270degree Pyramid Hologram Display Showcase Attractive, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chenille Microfiber Mop Head-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker]

Yashi 19 270degree Pyramid Hologram Display Showcase Attractive,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ysdcnc Manual Sheet Metal Plate Bending Roller Machine]

Yashi 19 270degree Pyramid Hologram Display Showcase Attractive, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Waterproof Pizza Sign Large Advertising Signboard, Part I.PVC Shrink Sleeve Label for Drinking Water Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light]

Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Galvanized Square Wire Mesh, Metal Window Screen.Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, 175-184[Hydraulic Motorspage]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube]

Whitaker,J.R.(1963),2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter. Analytical Chemistry35(12), 1950-1953[Dark Green Plastic PVC Film for Christmas Tree Leaves]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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