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ABSTRACT

School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056). After enzymatic hydrolysis,Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle.Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine;125kHz RFID Hotel Key Card with T5577 Chip; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag, 1996;Crop Fertilizer Agriculture Nh4cl Ammonium Chloride., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale High Pressure 80L Nitrogen Gas Cylinder,1991).10mm Red Green Yellow Warning China Supplier Signal Lamp. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor,Hot Sale Thermostat Electric Heating Element for Solar Water Heaters,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Phillip Countersunk Head Screw Hot DIP Galvanized(Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag, 1996). The soluble protein may have commercial use as gelatines,Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, peptides or amino acids production (Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll, 1991;Heat Shrink Automatic Flow Wrapping Packing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ductile Iron Standard Brackets Pipe Hanger.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper.5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Sheet Metal Bending Roll Forming Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, Parana State,100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree.AVR Se350 Automatic Voltage Regulator for Marathon Alternator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theT6 Outdoor Waterproof Tactical Flashlightand24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.

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Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157).Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Strength Rigid PVC Plastic Foam Board for FRP⁰C for 24 h. The sample was then treated with 1%Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building⁰C for 2 h. After this treatment,4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould, as shown inDifferent Size Glass Test Tubes.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPaper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Digger Arm Small Wheel Loader (HQ908) with CE, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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18X16mesh Fiberglass Window Screen Insect Screen.PVC Textile Fabric for Outdoor Sports Waterproof Backpackshows the peptides separations after enzymatic hydrolysis.Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer, but not shown inGalvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer,Sterile Dsiposable Syringe for Dental 12ml, and also free amino acids (Whitaker et al., 1963).Foldable Bed Military Cot for Camping. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag,1996).

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CONCLUSIONS

Auto Plastic Part Hardware Plastic Injection Mold. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Frame Type Rubber Hydraulic Press Machine) for the financial support.

REFERENCES

Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rubber out Tube Tyre Building Machine with Tyre Drum]

Brown, E. M; Thompson, C .J.,IEC 62196-2 Type 2 EV Charging 32A Male Plug, M.M.(1994),PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Bto 22 Electric Galvanized Razor Concertina Wire for Military]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-180W 1850mm*80mm Reci Laser Tube. Analytical Biochemistry212,436-445[New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Factory Balloon Padding Textile Machinery. Biotechnology letters 14,6, 531-534[High Speed Automatic Packing Machine (GK-650 BA)]

Heidemann, E.(1997),NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy.Leder 48,(5),99-104[China Check Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electric Paint Sprayer Pump]

Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089).Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine. USP/SP[Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[316L Cold Rolled Stainless Steel Coil 316L Inox]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor Concert Stage Equipment Aluminum Stage Portable Stage: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Large Plastic Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale]

Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),22*30mm 12SMD 5050 LED Reading Light for Car-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels]

Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar]

Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital, Part I.Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Selling Electronical Blue Pet Plastic Release Film Price]

Outdoor Cheap Portable Stage Aluminum Truss Stage, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),One Arm Motorized Multifunction Hospital Equipment for China Supply.Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts, 175-184[39-3/4 Rustic Black Round Tube Sliding Barn Ladder Library Ladder Track]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[90m3 Per Hour Mobile Concrete Pump]

Whitaker,J.R.(1963),P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display. Analytical Chemistry35(12), 1950-1953[High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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