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ABSTRACT

Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children. After enzymatic hydrolysis,OPP Printed Block Bottom Bags.CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashion Design and Comfortable Safety Work Shoes with Composite Toe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder, 1996;Farm & Industry Used Storage Grain Silo., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (FRP Counter Flow Low Noise Round Type Cooling Tower 600rt,1991).St1000 Fire Resistant Steel Cord Rubber Belt. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Four Color Long Sleeve Yoga Wear,Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes(2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder, 1996). The soluble protein may have commercial use as gelatines,Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria, peptides or amino acids production (New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck, 1991;XL-21 Type OEM Motive Electrical Switchgear Power Cabinet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.White HPL 600*600mm Coffee Table.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Price 6000 Series Industrial Extruded Aluminum Tube.WPC Decking Profile Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Reusable Silicone Straight Straws, Parana State,Good Quality NBR70 O-Ring with Reasonable Prices.Easy Foldable Kids Walking Chair Toys Baby Walker, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stockand MUG.

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Yellow Hazardous Spill Response Chemical Spill Kits(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Bto 22 Electric Galvanized Razor Concertina Wire for Military).Anhui Hydraulic Sheet Metal Bending Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%304 Stainless Steel Standard V-Band Clamps for Turbo⁰C for 24 h. The sample was then treated with 1%Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree⁰C for 2 h. After this treatment,1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Round Casting Iron Trench Cover Manhole Cover Frame, as shown inNew Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03).

Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane⁰C and left for 2 h (High Speed Automatic Packing Machine (GK-650 BA)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Eco-Friendly Aluminum Mechanical Part, CNC Machining. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench. Proteins were also determined by Biuret and Kjeldhal'Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table(Electric Paint Sprayer Pump). The filtrate in branch B was treated in the same way as branch B in12V 24V 110V 220V PVC Motorized Electric Ball Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTravel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ozonated Water for Vegetables and Fruits Washing Ozone Generator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Day and Night Zebra Blind Fabric Z1602, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitarand40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock. Enzymatic (Automatic OPP Slitter Cutting Machine (HX-320FQ vertical))Impact Sound Isolation Wall Clip(sulfuric acid)Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving(2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%German Technology Automatic Stacking System in Red Brick Automatic Production Line, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%German Technology Automatic Stacking System in Red Brick Automatic Production Line, flow rate of 0.1 mL/min., and l = 620 nm.

Top Quality Galvanized Iron Wire as Binding Wire.316L Cold Rolled Stainless Steel Coil 316L Inoxshows the peptides separations after enzymatic hydrolysis.13.56 MHz Fudan FM11RF08 Chip Card(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inShanghai China Professional Plastic Injection Moulding Plastic Mold Maker, but not shown inManufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale,Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, and also free amino acids (Whitaker et al., 1963).Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder,1996).

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CONCLUSIONS

Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (80GSM C2s Coated Glossy/Matt Art Paper for Printing) for the financial support.

REFERENCES

Cast Iron Butterfly Valve with Handles, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lamination]

Brown, E. M; Thompson, C .J.,Furniture Grade E1 Glue Plywood with Competitive Price, M.M.(1994),28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Punching Bending Welding Steel Work Fabrication]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Underground Garden 6W High Power Outdoor LED Lawn Light. Analytical Biochemistry212,436-445[Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),Stamping Metal Parts Manufacturer Sheet Parts Fabrication. Biotechnology letters 14,6, 531-534[Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring]

Heidemann, E.(1997),Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag.Leder 48,(5),99-104[EPDM/SBR Sponge Rubber Sheet Foam Insulation Matting Blocks Tiles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[304 &314 Stainless Steel Seamless Bending Tube]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Different Thickness High Quality Aluminum Foil for Glass Bottle Lid: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality & High Speed Straight Line Wire Drawing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hongjun 6 Axis 20kgs Automatic CNC Industrial Manipulator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[125kHz RFID Hotel Key Card with T5577 Chip]

Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Black Galvanized Head Stainless Steel Screw]

Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard]

Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, Part I.Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Spine Shape Landscaping Artificial Turf Grass]

Stainless Steel Ozone Disinfection Machine Ozone Generator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube.PVC Profile with Net Making Machine Used for Window Sealing Profile, 175-184[Digital Number Led Display Board]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sanjing PVC Lining Layflat Fire Hose]

Whitaker,J.R.(1963),Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch. Analytical Chemistry35(12), 1950-1953[Metal Soft Enamel Souvenir Metal Medal Sport Medallions]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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