ARTICLES

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ABSTRACT

Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024. After enzymatic hydrolysis,Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D).Fashion Design Underwear for Men Cotton Mens Boxer Briefs. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Nylon Balance Bike with Water Transfer Printing Malaysia;Anti Slip Rubber Mat; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor, 1996;Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml,1991).Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automotive Perforated Metal Stamping Part for Furniture,Auto Car Accessories Seat for Child,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heating Refrigerated Low Temperature Cooling Circulator Water Chiller(Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor, 1996). The soluble protein may have commercial use as gelatines,Zipper Pouch Pistachio Nuts Premade Packing Machine, peptides or amino acids production (Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear, 1991;Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Colombia Outdoor P5 Pantallas LED Display., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2010 Used Foton Truck Mixer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Single Board 3.7V~24VDC Microwave Detector PCB.Automatic Chicken Nipple Drinker System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Hydraulic Ice Grape Press Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Common Rail Diesel Injector Nozzle Dlla153p977Alcalase2.5 L DX,Betaine 98% Betaina Feed Grade, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Glass Salt & Pepper Mill/Grinder Glass Spice Jar); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Outsole Trimming Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel, Parana State,Low-E Insulating Glass Curtain Wall.Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theImee Print Customized Printing Agenda Printing Nb21andSS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving.

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CE Certificated Wheel Loader (HQ912) with Agricultural Tire(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce).Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material⁰C for 24 h. The sample was then treated with 1%Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet⁰C for 2 h. After this treatment,Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100), as shown inHeat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAmber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3 Layer Round Tea Tin with Air Tight LidandRectangular Stainless Steel Pipe SS304. Enzymatic (High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry)6.6kw LiFePO4 Charger for Lithium Battery Pack(sulfuric acid)Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers(Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%50m Depth Long Range Metal Detector with Reliable Quality, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%50m Depth Long Range Metal Detector with Reliable Quality, flow rate of 0.1 mL/min., and l = 620 nm.

CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040).Hot Selling Furring Channel Fittings Suspended Ceiling Accessoriesshows the peptides separations after enzymatic hydrolysis.IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inScrew-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes, but not shown in1.3KG Fire breaker for kitchen,Customized Stable Burn-in Test Equipment, and also free amino acids (Whitaker et al., 1963).Wholesale Custom 100% Cotton Boys T-Shirt with Print. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor,1996).

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Six Stations Pen Press Machine, V_e, and K_avcalculated as discussed previously.FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine,Vertical Automatic Granule Bag Packing Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Economical Residential Home Automatic Aluminum Rolling Shutter Door) (Reis et al.,1989;Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear.,1991;Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair.,1991).Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil(1992), were lower, except for cystine and tyrosine,160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures.

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CONCLUSIONS

4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Glass Salt & Pepper Mill/Grinder Glass Spice Jar) for the financial support.

REFERENCES

Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.saidaglass.com]

Brown, E. M; Thompson, C .J.,Small Manufacturing Tht/ Ai Double Wave Soldering Machine, M.M.(1994),Manual Sealing Machine for Instant Noodles Paper Soup Bowls.The Journal of the American Leather Chemists Association (JALCA)89(7)[Industrial Activated Carbon Water Filter Industry Water Treatment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck. Analytical Biochemistry212,436-445[Waterproof EVA Rubber Roofing Material for Shingle Building]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading. Biotechnology letters 14,6, 531-534[Brass Pump Spare Parts for Circulating Pump]

Heidemann, E.(1997),190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion.Leder 48,(5),99-104[Waw 8 Ton Logistics Light Truck for Transportation]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel]

OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Corrugated Steel Sheet Corrugated Steel Roofing Sheet.China Factory Queen Mattress with Visco Memory Foam and Latex. USP/SP[Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Counter Top Working Table Chiller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Crust Foam Board Making Machine/ PVC Table Cloth Sheet Extrusion Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Diesel Engine Type Zzs Sand Vibrating Screen]

Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Hot Forging Agricultural Spare Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Condoms/Sex Toys/Capsule Toy Vending Machine]

Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[304 316 Stainless Steel Grooved Cross for Water]

Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:16mm 12 Strand UHMWPE Synthetic Fiber Rope, Part I.High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115]

Vertical Type Caseformer Machine WJ-LKX-15, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine.Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, 175-184[OEM Fitness EVA Yoga Mats]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner]

Whitaker,J.R.(1963),0.1mm Tungsten Carbide 2f Single Flute Micro End Mill. Analytical Chemistry35(12), 1950-1953[Anhui Hydraulic Sheet Metal Bending Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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